A method for highly selective extraction of polysaccharides from mugwort

A high-selectivity, polysaccharide technology, applied in the production of bulk chemicals, can solve problems such as complicated processes, and achieve the effects of high extraction selectivity, high product purity, and shortened extraction time.

Active Publication Date: 2020-06-26
ANYANG INST OF TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"Extraction and purification method of mugwort polysaccharide (application number: 201110075571.8)" announced a method of extracting polysaccharide mugwort polysaccharide by ethanol solution percolation method, followed by dissolving in normal saline, repeatedly removing protein and desalting to obtain the finished product, the process is cumbersome

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  • A method for highly selective extraction of polysaccharides from mugwort
  • A method for highly selective extraction of polysaccharides from mugwort

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the making of standard curve of glucose and the detection method of polysaccharide

[0030] Use the phenol-sulfuric acid method to detect polysaccharides. First, draw a glucose standard curve: take the glucose standard product dried to constant weight at 105°C to prepare a mother solution with a concentration of 0.1 mg / mL, and perform stepwise dilution to prepare a final concentration of 0.01. , 0.02, 0.03, 0.04 and 0.05 mg / mL standard solutions; each take 2 mL of standard solutions of different concentrations, add 1 mL of 5% phenol solution and 5 mL of concentrated sulfuric acid in turn, mix well, and boil in water bath for 10 min, take out and Cool to room temperature, use distilled water as a blank, measure the absorbance at 490 nm, and record the data; take the glucose standard solution concentration as the abscissa, and the absorbance OD490 as the ordinate to make a standard curve ( figure 2 ), and fit the regression equation:

[0031] Y =13.205 x...

Embodiment 2

[0035] Example 2, traditional water extraction and alcohol precipitation method for polysaccharide extraction

[0036] Based on literature reports (Shen Xia, Zhang Yanhong, Yuan Huihui, et al. Optimization of Extraction Process of Artemisia argyi Folium Polysaccharides by Response Surface Method [J]. Chinese Patent Medicine, 2010, 32(1):48-51.) Optimized by Response Surface Method The optimal conditions for the extraction of crude polysaccharides from Artemisia argyi leaves were to extract polysaccharides from Artemisia argyi: the extraction temperature was 99°C, the extraction time was 2.3 h, and the ratio of water to material was 20. After the extraction, the extract was centrifuged at 5000 rpm for 15 min; the supernatant after centrifugation was concentrated under reduced pressure, and 4 times the volume of 95% ethanol solution was added, and placed in a refrigerator at 4 °C for 4 h. The polysaccharide is completely precipitated, and then filtered and freeze-dried to obtain...

Embodiment 3

[0038] Embodiment 3, the method used in the present invention extracts the polysaccharide of northern mugwort

[0039] Grind the leaves of Artemisia argyi with a water content lower than 8% and pass through a 40-mesh sieve, take 100 g and place them in an extraction tank, vacuumize until the vacuum degree in the extraction tank reaches -100 kPa, inject distilled water into the extraction tank as an extraction solvent, and Artemisia argyi samples were completely submerged, the mass volume ratio of leaves and distilled water was 1:8 g / ml, and the entrainer for extraction was [C 4 mim]Cl ionic liquid 8 g, the extraction temperature was controlled at 110°C by the automatic controller and the far-infrared generator at the bottom of the extraction tank, the far-infrared power was 1000 W, the wavelength range was 25-200 μm, the extraction pressure was 0.15 Mpa, and the extraction time 6 minutes, 2 times of circular extraction; after the extraction, the liquid solvent is pumped into t...

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Abstract

The invention discloses a method for extracting folium artemisiae argyi polysaccharide at high selectivity. The method comprises the following steps of 1, drying folium artemisiae argyi raw materials;then, crushing the folium artemisiae argyi raw materials into folium artemisiae argyi powder; 2, putting the folium artemisiae argyi powder into a sealed extraction tank; performing vacuum pumping toreach -100kPa; injecting distilled water to be used as an extraction solvent; adding an ion liquid carrying agent [C4mim]Cl for extraction; then, performing extraction, wherein the extraction temperature is controlled by an automatic controller; the heat source is a far infrared generating device arranged at the bottom of the extraction tank; 3, pumping a liquid solvent into an evaporating tank;performing constant-temperature reduced pressure desolvation treatment on the liquid solvent in the evaporating tank; obtaining a coarse extract after the liquid solvent desolvation; 4, performing dissolution by a 95-percent ethanol solution; putting the materials into a 4 DEG C refrigerator so that the polysaccharide is completely precipitated; then, performing filtering and freeze drying to obtain the folium artemisiae argyi polysaccharide. The method belongs to the extraction method with the advantages that the process is simple; the investment is low; the efficiency is high; the industrialproduction of high-quality folium artemisiae argyi polysaccharide can be realized.

Description

technical field [0001] The invention relates to the field of natural product extraction, in particular to a method for extracting polysaccharides from mugwort mugwort with simple process, high selectivity, high extraction rate and no damage to the activity of polysaccharides. Background technique [0002] North Ai ( Artemisia vulgaris L.) is a species of Artemisia argyi perennial herb of the genus Artemisia in the family Asteraceae. Li Shizhen's "Compendium of Materia Medica" in the Ming Dynasty stated: "(Ai) this herb is not native, but it grows in the field. In the Song Dynasty, Tangyin was used to restore the way (Note: that is now Fudao Town, Tangyin County) is the best... In modern times, only Tangyin is called Beiai". North Ai, Hai Ai, Qi Ai, and Qi Ai are collectively known as the "Four Famous Ai" in Chinese history. The whole herb of Bei Ai has the functions of warming Qi and blood, expelling cold and dampness, hemostasis, warming meridians, and preventing miscarr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003Y02P20/54
Inventor 蒋志惠张元臣张光杰张守泉祁鲲朱新亮张坤朋
Owner ANYANG INST OF TECH
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