Polylactic-acid-based bone tissue support loading icariin and deferoxamine and preparation method and application of polylactic-acid-based bone tissue support

A technology of icariin and tissue scaffolds, which is applied in the field of polylactic acid-based bone tissue scaffolds and preparations, can solve the problems of lack of mechanical properties and single improvement, and achieve a composition and performance that are easy to control, low in cost, and easy to industrialize Effect

Active Publication Date: 2018-06-01
广州倍健医疗用品有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Modification of deferoxamine on the surface of polylactic acid film is beneficial to the adhesion, spreading and proliferation of endothelial cells, and improves the angiogenesis ability of the material (Liu, Hua, etal. Materials Science and Engineering: C, 2017, 79: 399-409. ) However, the above studies only impro...

Method used

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  • Polylactic-acid-based bone tissue support loading icariin and deferoxamine and preparation method and application of polylactic-acid-based bone tissue support
  • Polylactic-acid-based bone tissue support loading icariin and deferoxamine and preparation method and application of polylactic-acid-based bone tissue support
  • Polylactic-acid-based bone tissue support loading icariin and deferoxamine and preparation method and application of polylactic-acid-based bone tissue support

Examples

Experimental program
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Effect test

Embodiment 1

[0042] (1) Prepare the PDLLA solution with chloroform to obtain an electrospinning solution with a concentration of 15% g / mL. After magnetic stirring and ultrasonic dispersion, the electrospinning solution is injected into the electrospinning solution supply device, and spinning is carried out under a static voltage of 20kV. The supply flow rate of the electrospinning solution is 2mL / h, and the distance between the receiving plate and the syringe pump needle is 15 cm to obtain PDLLA micron fiber scaffold.

[0043] (2) Dissolve trimethylaminomethane in deionized water, adjust the pH of the solution to 7 with 1mol / L hydrochloric acid while stirring, and prepare a 0.001mol / L trimethylaminomethane / hydrochloric acid buffer solution. Dopamine was dissolved in the above buffer solution, and a 1 g / L dopamine solution was obtained after magnetic stirring. Soak the PDLLA microfibrous scaffold in dopamine solution, react at room temperature and in the dark for 6 hours, then take out the...

Embodiment 2

[0047] (1) The PLLA solution was prepared using tetrahydrofuran solvent to obtain an electrospinning solution with a concentration of 25% g / mL. After magnetic stirring and ultrasonic dispersion, the electrospinning solution is injected into the electrospinning solution supply device, and spinning is carried out under a static voltage of 30kV. The supply flow rate of the electrospinning solution is 3mL / h, and the distance between the receiving plate and the syringe pump needle is 15 cm to obtain PLLA microfiber scaffolds.

[0048] (2) Dissolve trimethylaminomethane in deionized water, adjust the pH of the solution to 8 with 1mol / L hydrochloric acid while stirring, and prepare a 0.001mol / L trimethylaminomethane / hydrochloric acid buffer solution. Dopamine was dissolved in the above buffer solution, and a 2 g / L dopamine solution was obtained after magnetic stirring. The PLLA microfibrous scaffold was soaked in dopamine solution, reacted at room temperature and protected from ligh...

Embodiment 3

[0052] (1) The PLLA solution was prepared with toluene solvent to obtain an electrospinning solution with a concentration of 3% g / mL. After magnetic stirring and ultrasonic dispersion, the electrospinning solution was injected into the electrospinning solution supply device, and spinning was performed at a static voltage of 20 kV and a supply flow rate of 1 mL / h to obtain PLLA micron fiber scaffolds.

[0053] (2) Soak the PLLA microfibrous scaffold in a CS dilute acetic acid solution with a volume concentration of 5% acetic acid and a mass concentration of CS of 0.01%, for 12 hours. Then the scaffold was taken out and quenched at -140° C. for 1 h, and finally freeze-dried to obtain a PLLA / CS micro-nano composite fiber network scaffold with a hierarchical structure.

[0054] (3) Dissolve trimethylaminomethane in deionized water, adjust the pH of the solution to 8 with 1mol / L hydrochloric acid while stirring, and prepare a 0.001mol / L trimethylaminomethane / hydrochloric acid buffe...

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Abstract

The invention belongs to the field of a biomedical material, and specifically relates to a polylactic-acid-based bone tissue support loading icariin and deferoxamine, and a preparation and applicationof the polylactic-acid-based bone tissue support. Electrospinning technique is adopted to prepare a polylactic acid micrometer fiber support, which is then modified with a polydopamine layer, and finally, polydopamine is used for synergetic fixing of icariin and deferoxamine to the support. Through utilization of polydopamine, the hydrophilic performance, mechanical properties and cell affinity of the support are improved, and through cooperation of icariin and deferoxamine, the ossification performance and hematopoietic performance of the support are greatly improved. Through further adoption of thermally-induced phase separation, a chitosan nanometer fiber net is compounded on the polylactic acid micrometer fiber support, and based on the composite nanometer fiber net, polydopamine, icariin and deferoxamine are used for modification. The mechanical property of the support is obviously improved, which is beneficial for forming vasoganglion. The used materials are cheap, the adopted electrospinning and thermally induced phase separation technology are simple, and the performance of the material is easy to regulate, so that the support is suitable for industrialization.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a polylactic acid-based bone tissue scaffold loaded with icariin and deferoxamine, a preparation method and application thereof. Background technique [0002] Bone defects and bone injuries caused by various reasons are common clinical problems in medicine. Bone implantation is a common treatment for this problem, thus resulting in an urgent and huge market demand for bone repair materials. Polylactic acid has good biocompatibility, adjustable degradation rate and plasticity, so it has become an important class of bone repair materials. However, polylactic acid has disadvantages such as poor mechanical properties, poor hydrophilicity, lack of osteoconductivity and osteoinductivity, and no ability to angiogenesis, which severely limits its application as a bone tissue scaffold. Therefore, in order to overcome the above defects, on the one hand, it is necessary to ...

Claims

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Application Information

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IPC IPC(8): A61L27/18A61L27/54A61L27/50D01D5/00
CPCA61L27/18A61L27/50A61L27/54A61L2300/204A61L2300/232A61L2300/412A61L2300/45A61L2430/02D01D5/003C08L67/04
Inventor 罗丙红刘桦文伟周长忍
Owner 广州倍健医疗用品有限公司
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