Sparfloxacin molecular imprinting-quantum dot mesoporous material as well as preparation method and application thereof

A technology of molecular imprinting and sparfloxacin, which is applied in the analysis of materials, material excitation analysis, and material analysis through optical means, can solve problems such as disorder, and achieve fast reaction speed, easy elution, and mild reaction conditions. Effect

Active Publication Date: 2018-07-24
SOUTH CHINA NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been a few reports in this area, but most of them are disordered mesoporous structures

Method used

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  • Sparfloxacin molecular imprinting-quantum dot mesoporous material as well as preparation method and application thereof
  • Sparfloxacin molecular imprinting-quantum dot mesoporous material as well as preparation method and application thereof
  • Sparfloxacin molecular imprinting-quantum dot mesoporous material as well as preparation method and application thereof

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preparation example Construction

[0063] Such as figure 1 As shown, the present invention provides a method for preparing a sparfloxacin molecularly imprinted-quantum dot mesoporous material comprising the following steps: first, template molecule sparfloxacin, 3-aminopropyltriethoxysilane, propionic acid Thiomethylacetoxypropyltrimethoxysilane to form a pre-action complex; then, the Mn-doped ZnS quantum point to form a complex; finally, the template molecule sparfloxacin and the surfactant in the complex are eluted to obtain the sparfloxacin molecularly imprinted-quantum dot mesoporous material.

[0064] The present invention also provides the sparfloxacin molecularly imprinted-quantum dot mesoporous material prepared by the above preparation method and its application for detecting sparfloxacin in a sample.

[0065] Various test instruments and reagents in the embodiments of the present invention are commercially available, and can be purchased through commercial channels.

Embodiment 1

[0068] (1) Synthesis of thiomethylacetoxypropyltrimethoxysilane propionate (KH-3-MAP)

[0069] In a 10ml round bottom flask, 87.14 microliters of 3-mercaptopropionic acid (3-MAP) and 248.35 microliters of silane coupling agent γ-(methacryloyloxy)propyltrimethoxysilane (KH- 570) was dissolved in 5ml of absolute ethanol, and then the pH of the solution was adjusted to 8 with triethylamine, and stirred and reacted for 2 hours in a water bath at 40°C to obtain thiomethylacetoxypropyl trimethoxy propionate base silane (KH-3-MAP). The obtained product was stored in the refrigerator at 4°C for future use.

[0070] (2) Synthesis of L-cysteine-modified Mn-doped ZnS quantum dots (Mn:ZnS QDs)

[0071] S1: Weigh 0.1442g ZnSO with an analytical balance 4 ·7H 2 O, 0.0047g MnCl 2 4H 2 O and 0.1210gL-cysteine, and stir to make it dissolve in 25ml of ultrapure water, then add dropwise the freshly prepared molar concentration of 1M sodium hydroxide solution to adjust the pH of the mixed s...

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Abstract

The invention provides a preparation method of a sparfloxacin molecular imprinting-quantum dot mesoporous material. The preparation method comprises the following steps: firstly, preparing a preactioncompound from a template molecule sparfloxacin, 3-aminopropyl triethoxy silane and propionic acid sulfenyl methyl acetoxyl propyl trimethoxy silane; secondly, preparing a compound from a surfactant,a cross-linking agent, the preaction compound and L-cysteine modified Mn doped ZnS quantum dots; finally, deluting the template molecule sparfloxacin and the surfactant in the compound, thereby obtaining the sparfloxacin molecular imprinting-quantum dot mesoporous material. The invention further provides the sparfloxacin molecular imprinting-quantum dot mesoporous material prepared by using the preparation method, application of the material in detecting the content of sparfloxacin in a sample, and particularly application of the material in detecting the content of sparfloxacin in serum. Thepreparation method provided by the invention is simple in step, and by adopting the molecular imprinting-quantum dot mesoporous material, sparfloxacin in a sample can be rapidly, sensitively and specifically detected.

Description

technical field [0001] The invention belongs to the field of molecularly imprinted preparation, and relates to a molecularly imprinted-quantum dot mesoporous material, in particular to a sparfloxacin molecularly imprinted-quantum dot mesoporous material and its preparation method and application. Background technique [0002] Sparfloxacin (SPFX) is a third-generation quinolone antibacterial drug, which is widely used in veterinary medicine and human medicine to treat respiratory system, urinary system, digestive system, skin and soft tissue infections, etc. It is effective against Gram-positive bacteria, negative bacteria, Mycoplasma, mycobacteria, etc. have strong antibacterial activity. However, excessive use will cause environmental pollution and endanger human health. At present, the methods for detecting sparfloxacin mainly include spectrophotometry, electrochemical method, thin layer chromatography, high performance liquid chromatography, liquid chromatography-mass sp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/6432
Inventor 梁勇谭杰安耿园园汤又文杨军张嘉霖魏伟平
Owner SOUTH CHINA NORMAL UNIVERSITY
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