Dopamine-heparin-hyaluronic acid coating material capable of carrying and slowly releasing growth factors and preparation method of dopamine-heparin-hyaluronic acid coating material
A technology of hyaluronic acid and coating materials, which is applied in the field of pulp regeneration, can solve the problems of poor regeneration of pulp-dentin complex, regeneration of pulp-like tissue into odontocytes, and poor formation of dentin. Problems such as dentin cell layer and dentin formation are poor, achieving good biocompatibility, avoiding toxic and side effects, and simple process
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Embodiment 1
[0025]Taking dopamine hydrochloride, heparin and hyaluronic acid (purchased from Sigma Reagent Company, USA) at a molar ratio of 1:1:1 as an example: 201.6 mg of hyaluronic acid was dissolved in 50 mL of deionized water, 191.7 mg of EDC, 115.09 mg of NHS Slowly added to the solution, uniformly stirred for 20 min, and then added 94.82 mg of dopamine hydrochloride and 9.28 g of heparin to the above mixed solution. The reaction solution was protected under nitrogen and stirred at room temperature, adjusted to pH 4 with hydrochloric acid, kept for 3 hours, and then continued to react overnight at room temperature. After the reaction, the solution was purified by dialysis (molecular weight cut-off = 15000 Da) in an acidic environment of pH = 5 deionized water for 4 days, and dried in vacuum freezer (-70°C) for 72 hours to obtain a white flocculent graft product as dopamine-heparin- Hyaluronic acid coating material, the product quality is 234.16mg.
[0026] Change the feed ratio of...
Embodiment 2
[0031] Preparation of dentin tissue blocks: Freshly extracted premolars for orthodontic treatment were fully sterilized with 5% sodium hypochlorite aqueous solution and rinsed with PBS (phosphate buffered saline); then, the periodontal tissue was removed and cooled with PBS Cool down, obtain the tooth root, completely remove the pulp and early dentin, and cut to form 3.0mm*3.0mm*1.0mm dentin tissue blocks under water spray cooling; all dentin tissue blocks are soaked in deionized water for 5 hours in turn, and ultrasonically cleaned 20 minutes, soaked in 17% EDTA aqueous solution for 101 minutes, soaked in 19% citric acid aqueous solution for 1 minute, then sterilized with 5.25% sodium hypochlorite aqueous solution for 30 minutes, rinsed with PBS, and soaked for 2 days to obtain dentin tissue blocks.
[0032] The dopamine-heparin-hyaluronic acid coating material with a molar ratio of 1:1:1 was dissolved in 10mL deionized water at 25mg, 50mg, and 100mg respectively, and the conc...
Embodiment 3
[0036] Select the dopamine-heparin-hyaluronic acid solution with a molar ratio of 5:5:1 and a concentration of 2.5mg / mL, 5mg / mL, and 10mg / mL, and immerse the dentin tissue pieces prepared in Example 2 in the above-mentioned solution respectively, After 24 hours, it was taken out and spin-coated with a homogenizer (400r / s; 20s), and then transferred to a vacuum desiccator for freeze-drying for 48 hours. SEM observed the micro-morphological characteristics of the dentin surface coating, and the coating thickness was positively correlated with the concentration of dopamine-heparin-hyaluronic acid solution (Table 3).
[0037] Table 3: Dentin coating characteristics of different concentrations of dopamine-heparin-hyaluronic acid with a molar ratio of 5:5:1
[0038]
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