Dopamine-hyaluronic acid coating material, preparation method and its application in promoting specific adhesion of dental pulp stem cells to dentin

Abstract

The invention discloses a dopamine-hyaluronic acid coating material as well as a preparation method and application thereof in promoting specific adhesion of dental pulp stem cell to dentin, and belongs to the technical field of dental pulp regeneration. The preparation method comprises the following steps: sequentially adding raw materials including hyaluronic acid, 1-(3-dimethylamino propyl)-3-ethyl carbodiimide, N-hydroxyl succinimide and dopamine hydrochloride into deionized water according to a molar ratio of (1 to 10) to 2 to 2 to 1, and mixing uniformly; stirring at room temperature under nitrogen protection; adjusting the pH to 4-5 with hydrochloric acid or sodium hydroxide; reacting for 2-5 hours while keeping the pH value unchanged; reacting overnight at room temperature; finally, performing dialysis and purification on the obtained reaction solution for 3-5 days in an acid deionized water environment; collecting the product; performing vacuum freeze drying for 48-96h, wherein the obtained white flocculent grafted product is a dopamine-hyaluronic acid composite material. The coating thickness is controlled by changing the molar ratio of the raw materials as well as the product concentration, and the coating material can effectively promote specific adhesion of dental pulp stem cell to the root canal wall.

Description

technical field [0001] The invention belongs to the technical field of dental pulp regeneration, and in particular relates to a dopamine-hyaluronic acid coating material, a preparation method and its application in promoting specific adhesion of dental pulp stem cells to dentin. Background technique [0002] Pulp infection, trauma, and certain maxillofacial surgeries require the removal of infected pulp tissue, and the loss of living pulp leads to paresthesia, tooth discoloration, brittleness, and tooth splitting due to loss of nutrition in the hard tissue of the tooth , or even fracture or tooth extraction, etc., the complications of traditional endodontic treatment seriously endanger the patient's oral function, appearance and physical and mental health. Therefore, pulp regeneration has become a pulp therapy method with bright application prospects. Many scholars are competing to devote themselves to the research on pulp regeneration, and it has also become a research hots...

AI Technical Summary

Problems solved by technology

For example, some researchers have used tissue cell homing (Cell homing) or transplanted human stem cells from human exfoliated deciduous (SHED), dental pulp stem cells (Dental pulp stem cells, DPSC), human umbilical vein endothelial cells (Human umbilical vein endothelial cells) endothelial cells, HUVEC) as seed cells, growth factors such as TGF-β1, BMP-2, bFGF, VEGF, PDGF, NGF and BMP7, and platelet-rich plasma (Platelet–rich plasma, PRP), silk protein, PLLA microspheres , hyaluronic acid gel or collagen gel, etc. were used as scaffolds to study the pulp regeneration of full-length dental roots (or fragments), which can form pulp-like tissue and differentiate into odontoblasts. However, the odontoblast layer and The formation of dentin is poor, the regeneration effect of the pulp-dentin complex is not good, and the regenerated pulp-like tissue, especially the odontoblasts differentiated from seed cells such as DPSC, is not firmly combined with the dentin of the root canal wall, which ultimately hinders the regeneration of the dental pulp. regenerative development

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