Preparation method of mussel-imitated contact antibacterial hydrogel for tissue repair

A tissue repair and hydrogel technology, applied in medical science, surgery, etc., can solve problems such as bacterial resistance, failure of biomedical material implantation, unfavorable cell adhesion, etc., to increase tissue affinity, excellent reliability, etc. Restorative, enhanced antibacterial effect

Inactive Publication Date: 2018-08-07
SOUTHWEST JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Hydrogels are a class of highly hydrated materials that can be used in various medical applications, such as drug delivery, tissue engineering, wound fillers, and as implant coatings; meanwhile, a hydrated environment can also promote microbial infections; microbial infections can It leads to the damage of wound aggregation and the failure of implantation of biomedical materials; therefore, in addition to its main functional roles (such as wound healing, drug release, tissue repair and regeneration, etc.), hydrogels with antibacterial properties are more promising. ; The antibacterial properties of hydrogels come from two sources: antimicrobial agents (such as silver nanoparticles, antibiotics) and the inherent antibacterial properties of the hydrogel network; however, the abuse of antimicrobial agents can lead to bac

Method used

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  • Preparation method of mussel-imitated contact antibacterial hydrogel for tissue repair
  • Preparation method of mussel-imitated contact antibacterial hydrogel for tissue repair
  • Preparation method of mussel-imitated contact antibacterial hydrogel for tissue repair

Examples

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Example Embodiment

[0025] A method for preparing a mussel-like contact antibacterial hydrogel for tissue repair includes the following steps:

[0026] Step 1: Prepare mixed solution A of quaternary ammonium salt-modified natural polymer and methacrylamidated dopamine. The concentration of quaternary ammonium salt-modified natural polymer in mixed solution A is 1wt.%~8wt.%, methacrylamidated The dopamine concentration is 0.1wt.% ~1wt.%;

[0027] Step 2: Add anionic monomer and cationic monomer with a mass ratio of 9:1~7:3 to mixed solution A to form mixed solution B; the total concentration of anionic monomer and cationic monomer in mixed aqueous solution B is 5wt.% ~30wt.%;

[0028] Step 3: Add initiator and cross-linking agent to the mixed aqueous solution B, and a polymerization reaction occurs. After the reaction is completed, the desired hydrogel is formed.

[0029] Further, in the step 3, the polymerization reaction is carried out at a temperature of 60° C. and airtight conditions for 5-20 minutes...

Example Embodiment

[0037] Example 1

[0038] The preparation method of the imitation mussel contact antibacterial hydrogel for tissue repair is as follows:

[0039] Weigh 0.1g of quaternized chitosan and dissolve in 10ml of deionized water, stir thoroughly to form a transparent solution; weigh 0.015g of methacrylamidated dopamine and dissolve in the quaternized chitosan solution to form a mixed solution A ; Then weigh 1.75g ​​anionic monomer acrylic acid, 0.75g cationic monomer dimethylaminoethyl methacrylate, 0.08g sodium persulfate and 0.003g N, N-methylene bisacrylamide into the mixed solution A and stir uniformly to form Mixed solution B: Pour the mixed solution B into a closed mold, and react for 5 minutes at 60° C. to polymerize to form a hydrogel.

[0040] The hydrogel prepared according to the above method is subjected to compression test, figure 1 It can be seen from the figure that when the compressive strain reaches 85%, the hydrogel does not break and the compressive strength reaches 1.25M...

Example Embodiment

[0043] Example 2

[0044] The preparation method of the imitation mussel contact antibacterial hydrogel for tissue repair is as follows:

[0045] Weigh 0.7g of quaternized chitosan and dissolve in 10ml of deionized water, stir thoroughly to form a transparent solution; weigh 0.075g of methacrylamidated dopamine and dissolve in the quaternized chitosan solution to form a mixed solution A ; Then weigh 1.75g ​​anionic monomer acrylic acid, 0.75g cationic monomer dimethylaminoethyl methacrylate, 0.08g sodium persulfate and 0.003g N, N-methylene bisacrylamide into the mixed solution A and stir uniformly to form Mixed solution B: Pour mixed solution B into a closed mold and place it at 60°C for 15 minutes to polymerize to form a hydrogel.

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Abstract

The invention discloses a preparation method of mussel-imitated contact antibacterial hydrogel for tissue repair. The preparation method comprises the following steps: adopting methacryl amidated dopamine, anionic monomer and cationic monomer for copolymerization to generate triblock copolymer with opposite charges and mussel-imitated functional groups, doping quaternary ammonium salt modified natural polymer with positive charges into the triblock copolymer to prepare enhanced contact antibacterial ultra-strong polyelectrolyte hydrogel with a semi-interpenetrating network structure. The preparation method disclosed by the invention has the beneficial effects that due to existence of the cationic monomer and natural polymeric modified quaternary ammonium salt, the hydrogel has good antibacterial property; the methacryl amidated dopamine and the surfaces of bacteria generate mutual action to capture the bacteria, so that the antibacterial efficiency of the hydrogel is enhanced; simultaneously, the methacryl amidated dopamine can promote cell adhesion, so that the hydrogel has good cell affinity; in addition, the hydrogel prepared by the invention also has good mechanical property, stable swelling property and self-healing property and can be applied to tissue repair.

Description

Technical field [0001] The invention relates to biological functional materials, in particular to a method for preparing a mussel-like contact antibacterial hydrogel for tissue repair. Background technique [0002] Hydrogel is a kind of highly hydrated material, which can be used in various medical applications, such as drug delivery, tissue engineering, wound filler and as implant coating. At the same time, the hydrated environment can also promote microbial infection; microbial infection can Causes wound surface polymerization damage and biomedical material implantation failure; therefore, in addition to its main functions (such as wound healing, drug release, tissue repair and regeneration, etc.), hydrogels with antibacterial properties are more promising. ; There are two sources for the antibacterial properties of hydrogels: antibacterial agents (such as silver nanoparticles, antibiotics) and the inherent antibacterial properties of the hydrogel network; however, the abuse of...

Claims

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Application Information

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IPC IPC(8): A61L31/04A61L31/14A61L31/16C08J3/075C08F251/00C08F251/02C08F220/60C08F220/06C08F220/58
CPCA61L31/042A61L31/145A61L31/16C08F251/00C08F251/02C08J3/075A61L2300/232A61L2300/404C08J2351/02C08L51/02C08F220/603C08F220/585
Inventor 鲁雄徐僮甘东林
Owner SOUTHWEST JIAOTONG UNIV
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