Preparation method of SF-Cd sustained release microspheres by microwave synthesis

A slow-release microsphere and sf-cd technology, which is applied in microsphere preparation, microcapsule preparation, food science, etc., can solve problems such as poor tensile strength and decreased thermal stability of silk fibroin, and achieve good foaming stability Effect

Inactive Publication Date: 2018-08-10
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The results showed that after Na 2 CO 3 The thermal stability of silk fibroin degummed by solution is sig

Method used

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  • Preparation method of SF-Cd sustained release microspheres by microwave synthesis
  • Preparation method of SF-Cd sustained release microspheres by microwave synthesis
  • Preparation method of SF-Cd sustained release microspheres by microwave synthesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] The preparation method of embodiment 1 silk fibroin powder

[0060] The preparation method for making the silk fibroin powder of SF-Cd sustained-release microspheres, comprises the steps:

[0061] 1) Low temperature degumming

[0062] The traditional degumming method is more severe, requiring boiling at high temperature for several hours, and strong acid and strong alkali treatment. These methods will destroy the spatial structure of silk fibroin to a certain extent, resulting in the reduction of subsequent application performance and even the loss of its activity. . In this project, a milder low-temperature degassing treatment will be used to greatly retain the biological activity of silk fibroin.

[0063] After the impurities in the silkworm cocoon are removed and shredded, weigh a certain mass of silk fragments, and add deionized water and anhydrous sodium carbonate. The silk is completely submerged in the solution, stirred evenly, sealed and filled with nitrogen...

Embodiment 2

[0070] Embodiment 2 physical and chemical index analysis and characterization

[0071] (1) Determination of conventional ingredients

[0072] Determination of protein: Micro Kjeldahl method (GB5511-85)

[0073] Determination of fat: Soxhlet extraction method (GB5497-85)

[0074] Determination of moisture: 105 ℃ constant weight method (GB5512-85)

[0075] Determination of ash content: dry ashing method (GB5505-85)

[0076] (2) Determination of emulsification and emulsification stability

[0077] After being degraded by calcium chloride, silk fibroin will be endowed with good emulsification, foaming and stability. These good physical and chemical properties determine the application of silk fibroin in medical food and other fields [4] , so the research on the emulsification, foaming and stability of silk fibroin plays a decisive role in improving its application value.

[0078] A certain amount of silk fibroin is taken respectively to prepare silk fibroin solutions with a c...

Embodiment 3

[0235] Example 3 Structural characterization of SF-Cd and the influence of microwave on it

[0236] 1.1 Experimental materials

[0237] Table 4-1 Main experimental materials

[0238] Table 4-1 Main experiment materials

[0239]

[0240]

[0241] 2 Experimental methods

[0242] 2.1 Solubility of the product

[0243] Weigh a certain amount of product silk fibroin, add deionized water to dissolve, and prepare silk fibroin solutions with different pH values, stir at room temperature for 1 hour, centrifuge at 10000r / min for 10 minutes, take the supernatant, and use Coomassie brilliant blue method to determine the silk fibroin content in the solution.

[0244] 2.2 Fluorescence spectrum analysis

[0245] The silk fibroin, β-Cd, and microwave reaction products were reacted under the same conditions for 1 min, 2 min, 3 min, 4 min, 5 min, 6 min, and 7 min respectively, among which silk fibroin and β-Cd were used as the control group. After the reaction was completed, a solut...

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Abstract

The invention relates to a preparation method of SF-Cd sustained release microspheres by microwave synthesis. The method provided by the invention utilizes low temperature degassing treatment, degradation and purification separation to obtain silk fibroin, also adopts the silk fibroin as the modification object, carries out microwave wet process Maillard reaction to graft four carbohydrate substances respectively and performs screening, wherein cyclodextrin (Cd) has a highest grafting rate, and a silk fibroin glycosylation graft product can be obtained. The inventor explores the influence of different reaction factors on the silk fibroin free amino acid content and browning degree, at the same times adopts a plurality of characterization means to contrast the influence of microwave heatingand water bath heating on the physicochemical properties, structural functions and other aspects of SF-Cd covalent compounds. Finally, Left-Carvon (L-Ca) is adopted as the sustained-release model fororal cavity simulated sustained release performance test to determine the sustained release effect.

Description

technical field [0001] The invention relates to a preparation method of microwave-synthesized SF-Cd sustained-release microspheres. Background technique [0002] Chewing gum is a kind of leisure candy made of natural gum, syrup and flavors and fragrances through certain processing technology. Good quality chewing gum often takes a long time to chew, which requires the flavors and fragrances used to be chewable in the mouth and keep the release time lasting. Therefore, choosing a flavor with high concentration and low solubility is an important part of the chewing gum preparation process. Most of the solid flavors currently used in food have no slow-release performance or poor slow-release performance. Due to the advantages in production capacity and cost, most of the existing food flavors are prepared by spray drying and embedding method [66] , but the spray-dried powder encounters moisture during the eating process, and the capsule wall dissolves, making the core materia...

Claims

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Application Information

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IPC IPC(8): B01J13/14C08G81/00C08H1/00A23G4/06A23L27/00
CPCA23G4/06A23L27/00B01J13/14C08G81/00C08H1/00
Inventor 熊春华王文琦曹彦美朱明亮韩晓祥修丽丽蔡晓恬
Owner ZHEJIANG GONGSHANG UNIVERSITY
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