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Preparation method of algal polysaccharide derivatives

A technology of seaweed polysaccharide and derivatives is applied in the field of preparation of seaweed polysaccharide derivatives and achieves the effects of mild reaction conditions, low cost and simple operation

Inactive Publication Date: 2018-09-14
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few studies on the phosphorylation of Ulva polysaccharides at home and abroad, so the present invention establishes a method for phosphorylation modification of Ulva polysaccharides, and then obtains a new Ulva polysaccharide with high-efficiency antioxidant activity derivative

Method used

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  • Preparation method of algal polysaccharide derivatives
  • Preparation method of algal polysaccharide derivatives
  • Preparation method of algal polysaccharide derivatives

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Preparation of Ulva polysaccharides

[0032] (1) Pretreatment: Wash the Ulva algae body with tap water to remove silt, weeds and other attachments; then dry it naturally, pass through a 60-mesh sieve after crushing, and obtain the Ulva algae body powder, which is packaged for later use;

[0033] (2) Degreasing treatment: Weigh the Ulva pore algae body powder in step (1), reflux three times with 80% ethanol, centrifuge, and dry under reduced pressure at 40° C. to obtain degreasing Ulva pore algae body;

[0034] (3) Hot water extraction: extract the degreased Ulva pore algae bodies obtained in step (2) 3 times under the condition of a water bath at 125° C., extract 2 hours each time, filter, and combine the filtrates three times, then carry out rotary evaporation and concentration of the filtrates, and use 75% Precipitate with ethanol for 24h, filter out the precipitate;

[0035] (4) Drying: the precipitate filtered out in step (3) was dehydrated twice with absolute etha...

Embodiment 2

[0040] Preparation of Polysaccharide Derivatives from Ulva pore

[0041] (1) Ultrasonic treatment: add 1g of prepared Ulva polysaccharide into 100mL distilled water, add a certain amount of mixed phosphate reagent (STTP:STMP is 4:1), add 5g of anhydrous sodium sulfate, and use 200-400W power Ultrasonic treatment for 30 minutes to obtain a uniform mixed solution;

[0042] (2) pH adjustment: the mixed solution obtained in step (1) is adjusted to pH with newly prepared saturated sodium hydroxide solution or hydrochloric acid solution;

[0043] (3) Heating reaction: place the mixed solution after pH adjustment in step (2) in a three-necked flask, heat under reflux, and after heating for a certain period of time, cool to room temperature to obtain a reaction solution;

[0044] (4) Ethanol precipitation: Take the reaction solution in step (3), add 4 times the volume of the reaction solution with 95% ethanol and let it stand for 24 hours, remove the liquid, dry the precipitate, add ...

Embodiment 3

[0054] Determination of Hydroxyl Radical Scavenging of Phosphorylated Ulva Ulva Polysaccharide Derivatives

[0055] Take 150mmol / L sodium phosphate buffer solution (pH=7.4) 1mL, 360μg / mL saffron 1mL, 2mmol / LEDTA-Fe 0.5mL, add 1.0mL of the sample solution prepared according to the concentration of polysaccharide or polysaccharide derivative in Table 3, 3 %H 2 o 2 1 mL, shake well in a test tube, incubate and react in 37°C aqueous solution for 30 min, then measure the absorbance of the reaction system at 520 nm. Blank group: 1mL distilled water instead of the test sample. Control group: 1mL distilled water instead of the test sample, 1mL phosphate buffer instead of H 2 o 2 , the clearance results are shown in Table 3.

[0056] Calculation formula: clearance rate (%) = (A 样品 -A 空白 ) / (A 对照 -A 空白 )×100%

[0057] Table 3 Ulva pore polysaccharides and Ulva pore polysaccharide derivatives scavenging free radicals

[0058]

[0059] The experimental results show that, at d...

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Abstract

The invention relates to the technical field of natural polysaccharide chemical modification, in particular to a preparation method of algal polysaccharide derivatives. The method comprises the following steps of (1) ultrasonic pretreatment; (2) pH regulation; (3) heating treatment; (4) dialysis treatment; and (5) freeze drying. The method provided by the invention has the advantages that phosphate groups can be successfully guided into ulva pertusa polysaccharides to obtain an ulva pertusa polysaccharides phosphorylated derivatives with efficient anti-oxidization activity.

Description

technical field [0001] The invention relates to the technical field of chemical modification of natural polysaccharides, in particular to a preparation method of seaweed polysaccharide derivatives. Background technique [0002] Ulva pertusa (Ulva pertusa) belongs to Chlorophyta (Ulvaceae) Ulva genus, is a large green algae, widely distributed in Liaoning, Jiangsu, Shandong and other coastal areas in my country. As a traditional Chinese medicine, Ulva Ulva has the effects of lowering cholesterol, relieving heat, diuresis and decompression. The biological activity of polysaccharides is directly or indirectly affected by its structure. The prior art modifies the polysaccharide structure to change its activity. The in vitro antioxidant activity and antitumor effect of polysaccharides were further improved after phosphorylation modification. Currently commonly used polysaccharide structure modification methods include sulfation, phosphorylation, acetylation, alkylation, and car...

Claims

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Application Information

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IPC IPC(8): C08B37/04
CPCC08B37/0003C08B37/0084
Inventor 綦慧敏蒋南芳刘效磊李炳玺李伟达常新涛张羽
Owner WEIFANG MEDICAL UNIV
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