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A silk fibroin porous microsphere with nanofibrous microstructure and preparation method thereof

A technology of porous microspheres and nanofibers, which can be used in pharmaceutical formulations, prostheses, and drug delivery. It can solve the problems of insufficient material pores and lack of connectivity, and achieve cell phenotype maintenance, low cost, and excellent biocompatibility. Effects on Sexuality and Biodegradability

Active Publication Date: 2020-03-27
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Silk fibroin nanofibers are often made into membranes or scaffolds, which can promote wound healing and promote the rapid growth of bone tissue, vascular tissue cells and other structures, but they are often accompanied by problems such as insufficient pores or lack of connectivity.

Method used

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  • A silk fibroin porous microsphere with nanofibrous microstructure and preparation method thereof
  • A silk fibroin porous microsphere with nanofibrous microstructure and preparation method thereof
  • A silk fibroin porous microsphere with nanofibrous microstructure and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Shred silkworm cocoons, degumming and dissolving them, then filter, dialyze and concentrate to obtain 1.75% (w / v) silk fibroin aqueous solution;

[0023] 2) While stirring, add 1.35ml isopropanol dropwise to 10ml silk fibroin solution, stir for 5min to make it uniform, and let stand for 4d;

[0024] 3) Take 450ml of petroleum ether in a 500ml beaker and pre-cool at -50°C for 4h;

[0025] 4) Take another 40ml of petroleum ether into a 100ml beaker, add 0.7ml span-80, and stir evenly;

[0026] 5) Pour the mixed solution of 2) into the mixed solution of 4), and stir it into a uniform spherical shape, then quickly pour it into 3) the pre-cooled petroleum ether, and place it in a frozen environment for precipitation;

[0027] 6) Pour off the liquid in the solution of 5) under a freezing atmosphere, and then freeze-dry to obtain silk fibroin porous microspheres with a nanofiber microstructure.

[0028] The composition of the obtained porous microsphere is silk fibroin, t...

Embodiment 2

[0030] 1) Shred silkworm cocoons, degumming and dissolving them, then filter, dialyze and concentrate to obtain 7% (w / v) silk fibroin aqueous solution;

[0031] 2) While stirring, add 1.35ml of isopropanol dropwise to 10ml of silk fibroin solution, and stir for 5 minutes to make it uniform;

[0032] 3) Take 450ml of petroleum ether in a 500ml beaker and pre-cool at -50°C for 4h;

[0033] 4) Take another 40ml of petroleum ether into a 100ml beaker, add 0.7ml span-80, and stir evenly;

[0034] 5) Pour the mixed solution of 2) into the mixed solution of 4), and stir it into a uniform spherical shape, then quickly pour it into 3) the pre-cooled petroleum ether, and place it in a frozen environment for precipitation;

[0035] 6) Pour off the liquid in the solution of 5) under a freezing atmosphere, and then freeze-dry to obtain silk fibroin porous microspheres with a nanofiber microstructure.

Embodiment 3

[0037] 1) Shred silkworm cocoons, degumming and dissolving them, then filter, dialyze and concentrate to obtain 7% (w / v) silk fibroin aqueous solution;

[0038] 2) While stirring, add 1.35ml isopropanol dropwise to 10ml silk fibroin solution, stir for 5min, and let stand for 5d;

[0039] 3) Take 450ml of petroleum ether in a 500ml beaker and pre-cool at -50°C for 4h;

[0040] 4) Take another 40ml of petroleum ether into a 100ml beaker, add 0.7ml span-80, and stir evenly;

[0041] 5) Pour the mixed solution of 2) into the mixed solution of 4), and stir it into a uniform spherical shape, then quickly pour it into 3) the pre-cooled petroleum ether, and place it in a frozen environment for precipitation;

[0042] 6) Pour off the liquid in the solution of 5) under a freezing atmosphere, and then freeze-dry to obtain silk fibroin porous microspheres with a nanofiber microstructure.

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Abstract

The invention discloses a fibroin porous microsphere with a nano-fiber microstructure and a preparation method thereof. The preparation method comprises the following steps: after cutting silkworm cocoons into pieces, degumming and dissolving, filtering, dialyzing and concentrating to obtain a fibroin water solution with the concentration of 1.75 percent to 7 percent (w / v); under a stirring condition, dropwise adding isopropyl alcohol into the fibroin solution; stirring and standing; taking petroleum ether and pre-cooling at -30 DEG C to -50 DEG C for 3.5h or above and the like. The microsphere prepared by the preparation method has the nano-fiber microstructure and can be used for simulating a structure and a shape of an extracellular matrix and promoting cell attachment and proliferation; compared with a fibroin nano-fiber thin film or stent, the fibroin porous microsphere prepared by the preparation method has injectability and can avoid problems in a surgical operation process; meanwhile, three-dimensional culture of cells can be enlarged, the cell culture area is improved and cell phenotypes are maintained.

Description

technical field [0001] The invention relates to a silk fibroin porous microsphere with a nanofiber microstructure and a preparation method thereof, belonging to the field of polymer materials. Background technique [0002] The use of tissue engineering materials for cell culture is a hot research field at present, and the interaction between the material interface and the organism is an important topic. In organisms and in vitro culture, the main component of the extracellular matrix secreted by cells contains a large amount of protein material, and the matrix structure is nanofibers. Studies have found that biomaterials with micro-nano structures can stimulate and regulate cell behavior, and can enhance the compatibility of materials and tissues. Compared with other models such as membranes and scaffolds, microcarriers have a series of incomparable advantages in tissue engineering applications, such as their unique injectability. In addition, microcarriers can also provide...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/56A61L27/58A61L27/50A61L27/22A61L27/38
CPCA61L27/227A61L27/3895A61L27/50A61L27/56A61L27/58A61L2400/06C08L89/00
Inventor 杨明英雷芳
Owner ZHEJIANG UNIV
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