Absorbable wound repair material adsorbing ggtal gene knockout porcine collagen and preparation method thereof

A technology for wound repair and gene knockout, which is applied in the preparation methods of peptides, absorbent pads, chemical instruments and methods, etc., can solve the problems of fragility, easy detachment of dressing fibers, damage to new tissues, etc., to prevent melting and loss, maintain The effect of moisturizing wounds and preventing tissue adhesion

Active Publication Date: 2021-06-08
苏州明镜医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional dressings are the main dressings used clinically: their disadvantages are: they cannot keep the wound moist, and the wound healing is delayed; the dressing fibers are easy to fall off, causing foreign body reactions; Tissue damage; easy to cause exogenous infection during dressing change; heavy workload for dressing change
Synthetic dressings are usually made of materials such as polyvinyl alcohol, polyurethane, acrylamide, and carboxymethyl fiber. The main disadvantages are fragility, poor mechanical properties, low water vapor transmission rate, and a large amount of liquid retention, which can easily lead to accumulation under the membrane. Fluid aggravates infection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Extraction of Tendon Collagen

[0036] Collagen was extracted by combining trypsin digestion and acid extraction, the specific process is as follows:

[0037] (1) Purchase fresh GGTAl gene knockout pork tendons, remove muscle, fat, and remove fascia as much as possible, wash repeatedly with ultrapure water, and store in PE sample bags at -30°C; in a frozen state, use Cut the tendon into thin slices with a scalpel, and then mash it in a tissue masher; weigh about 60 grams of tendon crushed material, soak it in 0.05% (w / v) chlorhexidine acetate for 5 minutes, and then wash it with normal saline (0.9 % NaCl aqueous solution) to wash repeatedly, wash off residual chlorhexidine acetate as much as possible, and filter with gauze;

[0038] (2), at 37°C, soak the sterilized tendons in 0.25% trypsin / PBS (pH 7.4) digestion solution for 24 hours;

[0039] (3), after digestion, add 0.5% hydrogen peroxide (mass fraction 3%) of tendon quality and soak for 10-15 minutes, r...

Embodiment 2

[0044] Example 2 Preparation of Collagen Porous Sponge Scaffold

[0045] Dissolve the tendon collagen prepared in Example 1 in 3% (w / v) acetic acid solution (pH 2.78), stir evenly to form a collagen swelling solution with a concentration of 0.5% (w / v); after vacuum defoaming , frozen at -20°C for 1 hour, and finally freeze-dried in a freeze dryer for 24 hours to obtain a collagen porous sponge scaffold.

[0046] By changing parameters such as freezing temperature, freezing time, solution concentration and solution pH value, collagen porous sponge scaffolds with different microstructures can be obtained.

Embodiment 3

[0048] Preparation of Fibroin Porous Sponge Scaffold

[0049] Dissolve silk protein powder in 3% (w / v) acetic acid solution (pH 2.78), add glycerin to plasticize according to silk protein powder: glycerol mass ratio 10:1, stir evenly to form a concentration of 0.5% (w / v ) silk protein swelling solution; after vacuum defoaming treatment, freeze at -20°C for 1 hour, and finally freeze-dry in a freeze dryer for 24 hours to obtain a silk protein porous sponge scaffold.

[0050] By changing parameters such as freezing temperature, freezing time, solution concentration, and solution pH value, porous silk protein sponge scaffolds with different microstructures can be obtained.

[0051] Preparation of silk protein-collagen porous sponge scaffold

[0052] The planar collagen porous sponge scaffold made in Example 2 and the silk protein sponge scaffold are surface-to-surface laminated, and the sponge scaffold is further cross-linked with glutaraldehyde to improve stability. Steps: Soa...

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PUM

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Abstract

The invention discloses an absorbable wound repair material for adsorbing GGTAl gene knockout pig collagen. The GGTAl gene knockout pig tendon is used as a raw material to extract collagen; the collagen is dissolved in an acetic acid solution to obtain a collagen swelling solution, and the collagen swelling solution is vacuum defoamed After freezing treatment, the collagen porous sponge scaffold is obtained by freeze-drying in a freeze dryer; the absorbable material is made into a porous sponge scaffold, membrane or gel and then covered on the collagen porous sponge scaffold to make an absorbable wound repair material; or collagen Dissolved in acetic acid solution to obtain collagen swelling solution, then the absorbable material was made into a membrane and placed at the bottom of the collagen swelling solution. After vacuum defoaming, freezing treatment was performed, and freeze-dried in a freeze dryer to prepare absorbable wound repair material. The invention adopts GGTAl gene knockout porcine tendon to prepare wound repair scaffold, which can significantly reduce the scar formation after wound repair, and the collagen surface is covered with absorbable material, which is beneficial to the adsorption of collagen scaffold and prevents collagen from being melted and lost after encountering water.

Description

technical field [0001] The invention relates to an absorbable wound repair material which absorbs GGTA1 gene knockout pig collagen and a preparation method thereof. Background technique [0002] VSD technology is the product of combining the traditional negative pressure drainage method with closed negative pressure drainage filling dressing. It creates mechanical and biological effects by forming negative pressure on the wound surface to promote wound healing. Therefore, a special wound filling dressing must be used, which is a medical material that can temporarily protect the wound, prevent infection and promote healing. [0003] Artificial dressings can generally be divided into three categories: traditional dressings, synthetic dressings, and biological dressings. Traditional dressings are the main dressings used clinically: their disadvantages are: they cannot keep the wound moist, and the wound healing is delayed; the dressing fibers are easy to fall off, causing fore...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78A61L15/32A61L15/40A61L15/42A61L15/64A61L15/26A61L15/28A61L15/48C07K1/14
CPCA61L15/26A61L15/28A61L15/32A61L15/325A61L15/40A61L15/42A61L15/425A61L15/48A61L15/64A61L2300/412C07K14/78C08L67/04C08L5/08C08L5/04
Inventor 陈勇凌中华谈华平蔡腾谢发荣范文斌冯金柱
Owner 苏州明镜医疗科技有限公司
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