A kind of polyethylene glycol chemical hydrogel based on enzyme cross-linking and preparation method thereof
A polyethylene glycol and enzyme cross-linking technology, applied in the field of biomedical materials, can solve problems such as complex reaction processes, achieve the effects of improving modification rate, good biocompatibility, and broadening application prospects
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Embodiment 1
[0038] In a 1L three-necked flask, first add polyethylene glycol diacrylate (PEG400DA) and 1,4-dimercapto-2-butanol with a molar ratio of 1:1, then add 400mL dimethylsulfoxide, and stir until the magnet Dissolve completely; then measure 0.2 mL of triethylamine and dissolve it in 10 mL of dimethyl sulfoxide, and slowly inject it into the above reaction system through a micro syringe pump, and catalyze the Michael addition reaction at room temperature. After the system was reacted at room temperature for 24 hours, 0.40 g of N-isopropylacrylamide was added to end capping and the reaction was continued for 4 hours. After the reaction was completed, most of the dimethyl sulfoxide was removed by distillation under reduced pressure, and the crude product was settled in a large amount of toluene; after settled at -20°C for 24 hours, the upper toluene solution was carefully poured and vacuum dried for 24 hours to obtain linear polymer 1a. Using polystyrene as a standard sample, the numb...
Embodiment 2
[0040] In a 1L three-necked flask, first add polyethylene glycol diacrylate (PEG700DA) and dithiothreitol (DTT) with a molar ratio of 1:1, and then add 400 mL of dimethyl sulfoxide, and stir until the magnet is completely dissolved; Subsequently, 0.2 mL of triethylamine was measured and dissolved in 10 mL of dimethyl sulfoxide, and slowly injected into the above reaction system through a micro syringe pump, and the Michael addition reaction was catalyzed at room temperature. After the system was reacted at room temperature for 24 hours, 0.40 g of acrylamide was added for capping and the reaction was continued for 4 hours. After the reaction was completed, most of the dimethyl sulfoxide was removed by distillation under reduced pressure, and the crude product was settled in a large amount of toluene; after settled at -20°C for 24 hours, the upper toluene solution was carefully poured and dried under vacuum for 24 hours to obtain linear polymer 1bPEG700DA- DTT. Using polystyrene...
Embodiment 3
[0042] In a 1L three-necked flask, first add polyethylene glycol diacrylate (PEG1500DA) and 3,4-dimercapto-1-butanol with a molar ratio of 1:1, then add 400mL dimethylsulfoxide, and stir until the magnet Dissolve completely; then measure 0.2 g of sodium ethoxide and dissolve it in 10 mL of dimethyl sulfoxide, slowly inject it into the above reaction system through a micro syringe pump, and catalyze the Michael addition reaction at room temperature. After the system was reacted at room temperature for 48 hours, 0.40 g of N-isopropylacrylamide was added to cap the reaction and the reaction was continued for 8 hours. After the reaction was completed, most of the dimethyl sulfoxide was removed by distillation under reduced pressure, and the crude product was settled in a large amount of toluene; after 24 hours of settling at -20°C, the upper toluene solution was carefully poured and vacuum dried for 24 hours to obtain linear polymer 1c. Using polystyrene as a standard sample, the n...
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