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A microfluidic chip that captures rare blood cells

A microfluidic chip and chip technology, which can be used in pharmaceutical devices, enzymology/microbiology devices, bioreactors/fermenters for specific purposes, etc. Selective Offset, etc.

Active Publication Date: 2021-01-01
CHENGDU PRECISOME BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is generally believed that the microfluidic chip has the advantages of less sample volume and high sensitivity. However, due to the small amount of blood samples detected by each device, sample selectivity deviation is inevitable, and the incidence of false negatives remains high. have an important impact on clinical diagnosis
In addition, because the chip is often small in size, when the blood passes through, the flow rate is too small, the efficiency is too low, and at the same time, the possibility of coagulation is increased, and the flow rate is too high, resulting in a short binding time for the target component and insufficient capture. In addition, the shearing of blood cells The cutting force is also large, which may cause hemolysis. Therefore, the development of a microfluidic chip detection system with higher flow rate, better binding efficiency and lower hemolysis has become the focus of current research, so as to provide reliable data support for clinical practice.
On the other hand, some special chips are expensive and not easy to popularize and apply.

Method used

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  • A microfluidic chip that captures rare blood cells
  • A microfluidic chip that captures rare blood cells
  • A microfluidic chip that captures rare blood cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Embodiment 1: the making of chip

[0087] 1. Mask production: Output the chip mask with a high-precision laser printer, and the size of the mask is 5-10×2-4cm.

[0088] 2. Production and exposure of photosensitive film: use photosensitive film with a thickness of 35 μm / sheet to build a convex skeleton for chip design graphics, routinely use 3-13 photosensitive films, stick the mask on the photosensitive film, and irradiate with ultraviolet light for 30 seconds- Expose for 120 seconds; use cleaning solution and a soft brush to carefully remove the dissolved photosensitive film after exposure, use an oscillator to shake evenly during cleaning to ensure that the remaining dissolved photosensitive film is fully removed, wash 3 times with RO water; then put Dry in an oven for 10-30 minutes to obtain the convex skeleton of the chip design pattern; observe the chip skeleton under a stereo microscope to ensure that the edge of the chip skeleton is smooth and the tiny spoiler is...

Embodiment 2

[0100] Embodiment 2: Detection of functional parameters of the chip

[0101]Create the grid model of the chip design in the Gambit2.4 version software, and use the ANSYS Fluent version 19.0 software to simulate the flow state of the liquid fluid in the chip. It can be seen from the simulation data that ① the fluid flow distribution in each flow channel is uniform, and the T-shaped deflector guides the liquid in the buffer pool to each flow channel more uniformly; The total flow velocity and the fluid density are set to "WATER-LIQUID", and the calculated flow velocity in each flow channel is 0.026-0.199m / s; ③ Karman vortex street is formed behind each spoiler column (such as Figure 5 and Figure 6 shown). Meet the design requirements.

Embodiment 3

[0102] Example 3: Detection of capture ability of breast cancer circulating tumor cells

[0103] Insert the chip made in Example 1 into a container containing 10 ml of blood from a breast cancer patient. The blood enters the extracorporeal blood circulation system comprising the microfluidic chip set of the present invention after being guided out by blood vessels.

[0104] The microfluidic chipset composed of parallel / serial connection is connected through the medical-grade catheter, and the front-end anticoagulant slow-controlled release device is opened.

[0105] Turn on the power switch, and collect the cell suspension continuously for 1 hour.

[0106] Turn off the power switch of the device, turn off the automatic front-end anticoagulant slow-controlled release device, and the collection of circulating tumor cells ends.

[0107] Inject 200 μL of 1× PBS sterile solution to wash the chip 3 times.

[0108] Observation of circulating tumor cells and hemolysis

[0109] Usi...

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Abstract

The invention relates to the detection of biological and pathological body fluids, and relates to a microfluidic chip for separating high-purity rare cells from whole blood cell populations.

Description

technical field [0001] The invention relates to the detection of biological and pathological body fluids, and relates to a microfluidic chip for separating high-purity rare cells from whole blood cell populations. Background technique [0002] Rare cells refer to some atypical cells in biological fluid samples (including blood, pleural effusion, ascites, urine, cerebrospinal fluid, etc.). Studies have shown that the collection of rare cells and the use of them to complete NGS analysis have important guiding significance for finding potential therapeutic mechanisms, pathological mechanisms and targeted drug development of diseases. At present, the research methods for the detection of rare cells in blood mainly include flow cytometry, morphological separation, density gradient centrifugation, membrane filtration and immunomagnetic separation. For example, BDFACSAris can achieve high-speed cell sorting, but the instantaneous The laser will damage the sorted cells, and the cel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/00G01N33/569
CPCG01N33/56966C12M23/16C12M47/04A61M1/3679A61M2205/0244A61M2206/20
Inventor 孔祥菊石虎兵李燕雏徐琪
Owner CHENGDU PRECISOME BIOTECH CO LTD
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