Photoelectrochemical adaptor sensor as well as preparation method and application thereof

An aptamer sensor and photoelectrochemical technology, applied in the field of biosensors, can solve the problems of high detection cost, cumbersome operation, poor accuracy and specificity, etc., and achieve high light energy utilization rate, improved separation efficiency, and regular and orderly aperture Effect

Active Publication Date: 2018-11-20
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing methods for measuring OTC include: high performance liquid chromatography, enzyme-linked immunosorbent assay, capillary electrophoresis, immunoassay, and electrochemical methods, etc., but these methods have cumbersome operations, poor accuracy and specificity, and detection costs. Higher questions

Method used

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  • Photoelectrochemical adaptor sensor as well as preparation method and application thereof
  • Photoelectrochemical adaptor sensor as well as preparation method and application thereof
  • Photoelectrochemical adaptor sensor as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] A photoelectrochemical aptasensor, comprising a conductive glass electrode used as a working electrode in a three-electrode system, the reaction end surface of the conductive glass electrode is modified with a composite film, and the composite film is composed of phosphorous hybrid graphite phase nitrogen loaded with gold nanoparticles Composed of carbon nanosheets, the surface of the composite film is self-assembled with specific aptamer probes, which can specifically recognize and capture pollutant molecules, and the phosphorus hybrid graphite phase nitrogen loaded with gold nanoparticles Carbonized carbon nanosheets, using phosphorus hybridized graphite phase carbon nitride nanosheets as a carrier, and the surface of phosphorus hybridized graphite phase carbon nitride nanosheets is loaded with gold nanoparticles.

[0049] In this embodiment, the conductive glass electrodes are tin dioxide conductive glass electrodes.

[0050] In this embodiment, the mass ratio of pho...

Embodiment 2

[0065] A photoelectrochemical aptamer sensor, which is basically the same as the photoelectrochemical aptamer sensor in Example 1, the only difference being that the specific aptamer probe in the photoelectrochemical aptamer sensor in Example 2 is oxytetracycline specific Sexual aptamer probe, wherein the nucleotide sequence of oxytetracycline specific aptamer probe is 5'-(SH)-(CH 2 ) 6 -GGAATTCGCTAGCACGTTGACGCTGGTGCCCGGTTGTGGTGCGAGTGTTGTGTGGATCCGAGCTCCACGTG-3'.

[0066] A preparation method of the above-mentioned photoelectrochemical aptamer sensor of this embodiment is basically the same as the preparation method of Example 1, the only difference is: in the preparation method of Example 2, the specific adaptation used in step (5) The aptamer probe solution is an oxytetracycline-specific aptamer probe solution.

[0067] The photoelectrochemical aptasensor prepared in Example 2 is denoted as aptamer / Au / PCN-S / FTO.

[0068] An application of the photoelectrochemical aptasenso...

Embodiment 3

[0080] Investigate the detection accuracy of the photoelectrochemical aptasensor in Example 2, use the standard addition method to use the photoelectrochemical aptasensor for target detection in actual samples (refer to Example 2 for the measurement method), and perform a recovery experiment.

[0081] (1) Use the photoelectrochemical aptamer sensor in Example 2 to detect the concentration of oxytetracycline in the Xiangjiang River water respectively. The specific steps are: after pretreatment such as filtering the Xiangjiang River water, take the supernatant and use a phosphate buffer solution to adjust the pH to 7.0. The concentration of the target substance in the sample (containing oxytetracycline) refers to Table 1, and finally the photoelectrochemical aptasensor of embodiment 2 is used to detect oxytetracycline in the solution to be tested according to the method of embodiment 2, and the results are compared with traditional HPLC The test results of the methods were compa...

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Abstract

The invention discloses a photoelectrochemical adaptor sensor as well as a preparation method and application thereof. The photoelectrochemical adaptor sensor comprises a conductive glass electrode, wherein the reacting end surface of the conductive glass electrode is modified with a composite membrane consisting of phosphorus hybridization graphite phase carbon nitride nanosheets loading gold nanoparticles; and a specific adaptor probe is self-assembled on the surface of the membrane. The preparation method of the photoelectrochemical adaptor sensor comprises the following step: modifying thephosphorus hybridization graphite phase carbon nitride nanosheets loading the gold nanoparticles and the specific adaptor probe on the reacting end surface of the conductive glass electrode. The sensor disclosed by the invention has the advantages of high stability, long service life, high anti-jamming capability, wide detection range, low detection limit and the like. The preparation method of the photoelectrochemical adaptor sensor has the advantages of simple technology, convenient operation, safety, low cost, no pollution, high production efficiency and the like. The sensor disclosed by the invention can be widely applied to detecting pollutants (such as antibiotics) in mediums such as a water body and a living body and has the advantages of high utilization rate, wide application range, high application value and the like.

Description

technical field [0001] The invention belongs to the technical field of biosensors, and relates to a photoelectrochemical aptamer sensor and its preparation method and application. Background technique [0002] Due to the advantages of simple instrumentation, low cost, rapid analysis, and high sensitivity, photoelectrochemical sensors have attracted increasing research interest. In the photoelectrochemical sensor detection system, the working electrode is used to convert light energy into an electrical signal that is linearly related to the concentration, so the working electrode plays an important role in the photoelectrochemical sensor detection system. Various photoactive semiconductor materials such as TiO 2 , ZnO, CdS, and CdSe, etc., can effectively convert ultraviolet light or visible light, and have been widely used in the preparation of working electrodes, but these materials have low light utilization and contain metal ions that are potentially toxic, so it is nece...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30
CPCG01N27/308
Inventor 汤琳彭博欧阳细莲方思源邓垚成刘雅妮王敬敬
Owner HUNAN UNIV
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