Preparation method of bacterial cellulose immobilized laccase POPs subduction material

A technology of bacterial cellulose and immobilized laccase, applied to biochemical equipment and methods, chemical instruments and methods, fixed on/in organic carriers, etc., can solve the problems of not seeing POPs reduction materials, etc., and achieve The structure is controllable, the application is promoted, and the effect of high purity

Inactive Publication Date: 2018-11-23
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Up to now, there has been no related technology of using bacterial cellulose as immobilized laccase scaffold to prepare POPs reduction materials

Method used

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  • Preparation method of bacterial cellulose immobilized laccase POPs subduction material
  • Preparation method of bacterial cellulose immobilized laccase POPs subduction material

Examples

Experimental program
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Effect test

Embodiment 1

[0021] 1) Achromobacter was inoculated into the seed medium of pH 4.8 (by weight percentage, including: 30g / L glucose, 6g / L yeast extract, 10g / L peptone, 1g / L trisodium citrate, 2g / L magnesium sulfate ), under 26 ℃, 140r / min condition, carry out dynamic culture 36h days and make seed liquid, with the fermented medium of pH6.8 with the 15% inoculum of volume percentage, seed liquid is transferred to (by weight percentage, comprising: 30g / L glucose, 12g / L yeast extract, 12g / L tryptone, 2g / L sodium citrate, 2g / L magnesium sulfate, 2.7g / L Na 2 HPO 4 , 2g / L KH 2 PO 4 ), each 250mL Erlenmeyer flask contained 50mL of fermentation broth, cultured statically at 26°C for 8 days, and obtained a bacterial cellulose film on the upper layer of the culture broth;

[0022] 2) Take out the bacterial cellulose film obtained in step 1), rinse slowly with deionized water several times, remove the bacterial cells and residual culture medium on the surface of the film, and use a mass fraction o...

Embodiment 2

[0025]1) Inoculate Alcaligenes into the seed culture medium of pH 6.8 (by weight percentage, including: 30g / L glucose, 6g / L yeast extract, 10g / L peptone, 1g / L trisodium citrate, 2g / L magnesium sulfate ), under the conditions of 30°C and 160r / min, carry out dynamic culture for 30h to obtain the seed liquid, and transfer the seed liquid to the fermentation medium of pH 6 with the volume percentage 8% inoculum (in weight percentage, comprising: 30g / L Glucose, 12g / L yeast extract, 12g / L tryptone, 2g / L sodium citrate, 2g / L magnesium sulfate, 2.7g / L Na 2 HPO 4 , 2g / L KH 2 PO 4 ), each 250mL Erlenmeyer flask contained 70mL of fermentation broth, cultured statically at 30°C for 7 days, and obtained a bacterial cellulose film on the upper layer of the culture broth;

[0026] 2) Take out the bacterial cellulose film obtained in step 1), rinse slowly with deionized water several times, remove the bacterial cells and residual culture medium on the surface of the film, and use a mass fr...

Embodiment 3

[0029] 1) Inoculate the non-rhizobia into the seed culture medium of pH 5 (by weight percentage, comprising: 30g / L glucose, 6g / L yeast extract, 10g / L peptone, 1g / L trisodium citrate, 2g / L magnesium sulfate ), under 28 ℃, 200r / min condition, carry out dynamic culture 20h day and make seed liquid, with the fermented medium of pH 4.8 (in weight percentage, comprise: 30g / L Glucose, 12g / L yeast extract, 12g / L tryptone, 2g / L sodium citrate, 2g / L magnesium sulfate, 2.7g / L Na 2 HPO 4 , 2g / L KH 2 PO 4 ), each 250mL Erlenmeyer flask contained 60mL of fermentation broth, cultured statically at 27°C for 6 days, and obtained a bacterial cellulose film on the upper layer of the culture broth;

[0030] 2) Take out the bacterial cellulose film obtained in step 1), rinse slowly with deionized water several times, remove the bacterial cells and residual culture medium on the surface of the film, and use a mass fraction of 0.1mol / L NaOH solution in a water bath at 85°C Treat for 90 minutes u...

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Abstract

The invention discloses a preparation method of a bacterial cellulose immobilized laccase POPs subduction material. The preparation method is characterized by comprising the following steps: transferring an inclining bacterial strain into a seed culture medium, performing the dynamic culture, then performing the transferring static culture, and obtaining a bacterial cellulose membrane film; processing 0.1 percent NaOH in a water bath, flushing by using deionized water until the neutrality, freeze drying, and obtaining a cellulose dry membrane; and finally preparing the immobilized laccase material by adopting an adsorption-cross linking method, wherein the immobilized laccase material can be used for processing a durability organic pollutant in natural water. The method is simple in operation, safe, environment-friendly and high in controllability. By adopting the bacterial cellulose film prepared in the invention, the laccase can be safely and efficiently immobilized, the enzyme activity can be maximally kept, the problems of the traditional free laccase for degrading the POPs pollutants in the water such as severe loss, difficulty in reusability and the like can be avoided, and the POPs in the natural water can be efficiently and safely degraded and eliminated.

Description

technical field [0001] The invention relates to a preparation method of a POPs reduction material, in particular to a preparation method of a bacterial cellulose immobilized laccase POPs reduction material, and belongs to the technical field of environmental protection engineering. Background technique [0002] Persistent Organic Pollutants (POPs) are toxic, bioaccumulative and semi-volatile, persist in the environment, and can migrate long distances through various environmental media (such as the atmosphere, water, and organisms). Natural or artificial organic pollutants that ultimately affect human health and environmental safety. Due to the chemical stability of cyclic polymer compounds, POPs are difficult to be decomposed by anaerobic or aerobic microorganisms in the biochemical link of conventional wastewater treatment systems, and most of them still remain in industrial wastewater, and eventually flow into the ecosystem with external drainage. The continuous enrichme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/12C12P1/04C12N9/02C02F3/34
CPCC02F3/342C12N9/0061C12N11/12C12P1/04C12Y110/03002
Inventor 张勇徐素芹潘建义姚菊明
Owner ZHEJIANG SCI-TECH UNIV
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