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Manuka honey identification method based on nuclear magnetic resonance technology

A manuka honey and nuclear magnetic resonance technology, which is applied in the fields of nuclear magnetic resonance analysis, material analysis through resonance, instruments, etc., can solve problems such as impact, and achieve short analysis time, strong controllability, and good specificity Effect

Active Publication Date: 2018-11-23
南京海关动植物与食品检测中心 +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, the current domestic research on manuka honey is basically blank, which will have a great impact on the supervision and supervision of imported manuka honey and the protection of the legitimate rights and interests of Chinese consumers

Method used

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  • Manuka honey identification method based on nuclear magnetic resonance technology
  • Manuka honey identification method based on nuclear magnetic resonance technology
  • Manuka honey identification method based on nuclear magnetic resonance technology

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Determination of Characteristic Markers

[0033] 1. Instruments and equipment

[0034] Experimental equipment includes: Advance 400 nuclear magnetic resonance instrument (Bruker, Switzerland), 14.1 T superconducting magnet, 5 mm dual-core z-gradient probe and Topspin 2.3 test control and data processing software; 5 mm nuclear magnetic resonance sample tube; high-speed centrifuge (Sigma , German company); Vortex mixer (XW-80A, Shanghai Medical University Instrument Factory); LP403 analytical balance (Sartorius, German company);

[0035] Experimental solvents include: deuterated water (99.8% deuterium band) purchased from Cambridge Isotope Laboratories; 3-trimethylsilyl-1-propyl sodium sulfonate (TSPSA) purchased from Aldich-Sigma; dihydrogen phosphate Potassium and sodium dihydrogen phosphate (excellent grade) were purchased from Aldich-Sigma.

[0036]2. Honey Sample Collection

[0037] A total of 7 varieties of manuka honey, kanuka honey, rivariwa honey, ka...

Embodiment 2

[0048] The samples submitted for inspection are respectively labeled as 1, 2, 3, and 4.

[0049] Pretreat the sample according to its nature, specifically:

[0050] For commercially available honey samples, stir them evenly, accurately weigh 0.25 g of samples into a centrifuge tube, add 1 mL of heavy water, and dissolve completely; take 0.8 μl of the above solution, add 100 μl of 0.5 mol / L phosphate buffer solution (pH =2.0), wherein the buffer solution contains 0.05% volume fraction of 3-trimethylsilyl-1-propyl sodium sulfonate internal standard solution and 0.1 mM sodium azide, vortexed to mix evenly, and then Use 1.0 mol / L hydrochloric acid and sodium hydroxide to adjust the pH of the final solution to 2.5, and take 600 μl of it in an NMR tube.

[0051] For the honey sample, stir it evenly, filter it with a nylon filter cloth with a pore size of 0.10mm-0.14mm, remove the solid impurities in the honey, accurately weigh 0.25 g of the sample in a centrifuge tube, add 1mL of h...

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Abstract

The invention belongs to the field of food safety, particularly belongs to the field of Manuka honey identification, and specifically relates to a Manuka honey identification method based on the nuclear magnetic resonance technology. According to the method, nuclear magnetic resonance spectrum data of to-be-detected samples are analyzed to determine whether the to-be-detected samples are the Manuka honey or not. The Manuka honey identification method has the advantages that through specific characteristic markers, defects in current Manuka honey identification can be overcome; the method has the advantages of short analysis time, high integrity, high specificity, high controllability, high accuracy and the like; by the method, the minimum detection quantity of the Manuka characteristic markers in the Manuka honey is 10%.

Description

technical field [0001] The invention belongs to the field of food safety, in particular to the field of manuka honey identification, and more specifically relates to a method for identifying manuka honey based on nuclear magnetic resonance technology. Background technique [0002] Manuka (Manuka) honey is a precious honey species unique to New Zealand. It is a shrub plant unique to New Zealand - Manuka ( Leptospermum scoparium ) brewed from nectar. Because Manuka honey has unique antibacterial activity, it can be used to prevent wound infection, help wound healing, and has high medicinal value, so it is more and more favored by Chinese consumers. [0003] At present, New Zealand's manuka honey exports have increased by about 10% every year for the past 10 years. In 2013, the value of honey exported from New Zealand was 128 million New Zealand dollars. According to statistics from a New Zealand beekeeper association, New Zealand only produces about 1,700-2,000 tons of Manuk...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N24/08
CPCG01N24/082
Inventor 吴斌刘芸丁涛沈伟健张建费晓庆陈磊陆慧媛刘艳黄娟
Owner 南京海关动植物与食品检测中心
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