Lactobacillus acidophilus composite agent and preparation method and application thereof

The technology of Lactobacillus plantarum and compound agent is applied in the directions of biochemical equipment and methods, lactobacillus, microorganism-based methods, etc., and can solve the problems of insignificant probiotic effects such as enhancing immunity, insufficient stability, and insufficient number of viable bacteria, etc., Achieve the effect of shortening fermentation time, improving storage time and improving stability

Inactive Publication Date: 2018-11-30
诺佰克(武汉)生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the problems in the prior art that the number of live bacteria in the Lactobacillus plantarum preparation is not high enough, the stability is insufficient, and the probiotic effect of enhancing immunity is not significant, the present invention provides a Lactobacillus plantar compound agent, wherein the Lactobacillus plantarum and proteolysis There is a synergistic effect of peptides, which can significantly improve the probiotic effect of Lactobacillus plantarum

Method used

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  • Lactobacillus acidophilus composite agent and preparation method and application thereof
  • Lactobacillus acidophilus composite agent and preparation method and application thereof
  • Lactobacillus acidophilus composite agent and preparation method and application thereof

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preparation example Construction

[0047] The present invention also provides a preparation method of the plantarum lactobacillus compound described in the above-mentioned technical scheme, comprising the following steps:

[0048] (1) inoculating Lactobacillus plantarum into the seed medium for activation to obtain a primary seed liquid;

[0049] (2) inoculating the primary seed liquid into the seed medium for anaerobic cultivation for 8-24 hours to obtain the secondary seed liquid;

[0050] (3) inserting the secondary seed liquid into a fermentation medium containing proteolytic polypeptides for high-density fermentation, centrifuging to collect precipitates, and obtaining bacterial sludge;

[0051] (4) emulsifying embedding after mixing the bacteria slime and the freeze-drying protective agent to obtain an emulsion;

[0052] In terms of mass percentage, the freeze-drying protective agent includes 10-30% of skim milk powder, 10-15% of proteolytic polypeptide and the rest of water;

[0053] (5) The obtained e...

Embodiment 1

[0086] In terms of mass percentage, seed medium: 2% glucose, 1% peptone, 0.5% yeast extract, 0.5% anhydrous sodium acetate, 0.06% magnesium sulfate, 0.02% manganese sulfate, 0.1% Tween 80 and the rest of water , pH value 6.5 ~ 6.8;

[0087] In terms of mass percentage, fermentation medium: 10% egg white protein hydrolyzate (by dry weight, 3% egg white protein hydrolyzate polypeptide), 2% glucose, 1% peptone, 0.5% yeast extract, 0.5% anhydrous acetic acid Sodium, 0.06% magnesium sulfate, 0.02% manganese sulfate, 0.1% Tween 80 and the balance of water, the pH value is 6.2;

[0088] After detection, the polypeptide content in the egg white proteolytic hydrolyzed polypeptide is 82%, the distribution ratio of relative molecular weight 1000Da is 87%, the mass percentage of aspartic acid is 1.8%, and the mass fraction of glutamic acid is 9.3%. In terms of mass percentage, the freeze-drying protective agent: 10% of skim milk, 10% of enzymatic hydrolyzate of egg white protein, and the...

Embodiment 2

[0091] In terms of mass percentage, seed medium: 2% glucose, 1.2% peptone, 0.5% yeast extract, 0.5% anhydrous sodium acetate, 0.02% magnesium sulfate, 0.06% manganese sulfate, 0.1% Tween 80 and the rest of water , pH value 6.5 ~ 6.8;

[0092] In terms of mass percentage, fermentation medium: 0.5% bitter gourd seed protein enzymatic hydrolysis dry powder, 2% glucose, 1% peptone, 0.5% yeast extract, 0.5% anhydrous sodium acetate, 0.06% magnesium sulfate, 0.02% manganese sulfate, 0.1% Tween 80 and the balance of water, the pH value is 6.4;

[0093] After testing, the polypeptide content of the bitter melon seed protein enzymatic hydrolyzed polypeptide is 78%, the distribution ratio of the relative molecular weight of 1000Da is 83%, the mass percentage of aspartic acid is 1.2%, and the content of glutamic acid is 5.8%.

[0094] In terms of mass percentage, the freeze-drying protective agent: skimmed milk 10%, balsam pear seed protein enzymatic hydrolyzed polypeptide dry powder 15...

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Abstract

The invention relates to the field of microbial composite preparation, and provides a lactobacillus plantarum composite agent. The lactobacillus plantarum composite agent includes preparation raw materials of 85-90 parts of lactobacillus plantarum and 1.5-6 parts of proteolytic polypeptide; the number of living bacteria in the lactobacillus plantarum composite agent is 10<10>cfu/g or more; the mass content of the polypeptides in the proteolytic polypeptide is 75% or more, the mass fraction of the polypeptides with relative molecular weight less than 1000Da is not less than 80%, the mass fraction of aspartic acid in the proteolytic polypeptide is 1% or more, and the mass fraction of glutamic acid in the proteolytic polypeptide is 5% or more. The study shows that after combination of the lactobacillus plantarum and the proteolytic polypeptide, the antioxidant, antiaging, hyperglycemic, hypolipidemic and immunity-enhancing functions of the lactobacillus plantarum can be significantly improved. In the preparation process, the proteolytic polypeptide can also play a role in promoting the proliferation of the lactobacillus plantarum to shorten the fermentation time and freeze-drying protection.

Description

technical field [0001] The invention relates to the field of microbial compound preparations, in particular to a plant lactobacillus compound preparation and its preparation method and application. Background technique [0002] Lactobacillus plantarum is a microorganism of the genus Lactobacillus in the Lactobacillus family, with an optimum growth temperature of 30-35°C, anaerobic or facultative anaerobic, and the strains are straight or curved rods, single, sometimes in pairs or in chains , the optimum pH is around 6.5, belonging to homofermentative lactic acid bacteria. [0003] Lactobacillus plantarum is a lactobacillus that has been proven to have various physiological functions such as enhancing immunity, inhibiting pathogenic bacteria, lowering serum cholesterol levels, and preventing cardiovascular diseases. It is used in products such as yogurt, fruit and vegetable juice fermentation, and functional probiotic preparations. be widely used. [0004] However, Lactobac...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A23L33/135A23L33/18A23L2/52A23L21/10A23G3/36A23G3/44A23G1/42A23G1/44A21D8/04A21D2/24C12R1/25
CPCA21D2/245A21D8/04A23G1/423A23G1/44A23G3/366A23G3/44A23L2/52A23L21/10A23L33/135A23L33/18C12N1/04C12N1/20A23V2002/00A23V2200/30A23V2200/328A23V2200/3262A23V2200/324A23V2250/55A23V2400/169
Inventor 张阳潘芬杨帆何慧玲湛剑龙
Owner 诺佰克(武汉)生物科技有限公司
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