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Dibenzyl dimer compound and method and application for extracting and separating compound from dendrobium stems

A technology of bibenzyl dimer and Dendrobium nobile can be used in organic chemistry, drug combination, antineoplastic drugs, etc., and can solve problems such as complex and diverse chemical components

Active Publication Date: 2018-12-28
ZUNYI MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Dendrobium nobile is known as "folk fairy grass and plant gold" because of its pharmacological effects, but its chemical composition is complex and diverse, mainly containing alkaloids, aromatics and sesquiterpenoids. The chemical constituents of Dendrobium nobile, and the development of active constituents in Dendrobium nobile is exactly the subject of urgent research.

Method used

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  • Dibenzyl dimer compound and method and application for extracting and separating compound from dendrobium stems
  • Dibenzyl dimer compound and method and application for extracting and separating compound from dendrobium stems
  • Dibenzyl dimer compound and method and application for extracting and separating compound from dendrobium stems

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Effect test

Embodiment 1

[0027] The method for extracting and separating the above-mentioned bibenzyl dimer compounds from Dendrobium nobile of the present invention comprises the following steps:

[0028] 1) Take 5.0 kg of dried stems of Dendrobium nobile, after pulverization, reflux extraction with 95% ethanol for 4 times, combine the extracts for 4 times, and concentrate the extracts under reduced pressure to obtain 0.5 kg of ethanol extract;

[0029] 2) Disperse the ethanol extract in step 1) with 0.5% hydrochloric acid aqueous solution and stir thoroughly, then filter to obtain the acid-water insoluble matter. The acid water-insoluble matter was sequentially extracted 4 times with petroleum ether, petroleum ether: ethyl acetate 1:1 volume, and ethyl acetate respectively, and concentrated under reduced pressure to obtain extracts of various parts;

[0030] 3) Separate the extract from the ethyl acetate part in step 2) by silica gel column chromatography, and wash with petroleum ether-ethyl acetate...

Embodiment 2

[0033] Embodiment 2: The difference with Embodiment 1 is that

[0034] The concentration of aqueous ethanol used for reflux extraction in step 1) is 85% for reflux extraction twice; step 2) for extraction twice; step 4) for methanol with a concentration of 95%; step 5) for acetonitrile with a concentration of 50%, The detection wavelength is 240nm, 260nm. All the other steps are the same as in Example 1, and the beneficial effects described in the present invention can also be obtained.

Embodiment 3

[0035] Embodiment 3: The difference with Embodiment 1 is that

[0036] The concentration of aqueous ethanol used for reflux extraction in step 1) is 70% reflux extraction for 4 times; step 2) for extraction 4 times; step 4) with a concentration of 90% methanol; step 5) with a concentration of 70% acetonitrile, The detection wavelength is 240nm, 260nm. All the other steps are the same as in Example 1, and the beneficial effects described in the present invention can also be obtained.

[0037] The present invention carries out the condition of TLC identification: developer is sherwood oil-ethyl acetate system and dichloromethane-methanol system, chromogenic agent a: observe fluorescence under ultraviolet lamp (254nm); chromogenic agent b: iodine cylinder color development .

[0038] Structural identification: The spectroscopic techniques used mainly include nuclear magnetic resonance spectroscopy ( 1 H-NMR, 13 C-NMR, HSQC, HMBC) and mass spectrometry (HR-ESI-MS) identified t...

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PUM

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Abstract

The invention discloses a dibenzyl dimer compound and method and application for extracting and separating the compound from dendrobium stems in the technical field of plant effective ingredient extraction. The method is characterized in that an extraction method and a column chromatography are adopted to enrich ingredients, a method for refining with a modern chromatography separating method is adopted to separate and purify the complicated ingredients from the dendrobium stems. By adopting the provided preparation method, a dibenzyl dimer is successively extracted and separated from the dendrobium stems, and the dibenzyl dimer has very good antitumor in-vitro cellular activity and can be used for preparing antitumor drugs.

Description

technical field [0001] The invention relates to the technical field of plant active ingredient extraction, in particular to a bibenzyl dimer compound and its extraction and separation method and application from Dendrobium nobile. Background technique [0002] Dendrobium nobile Lindl., a plant of the genus Dendrobium orchidaceae, is a traditional precious Chinese medicine in China and an authentic medicinal material in Guizhou. Among them, Dendrobium nobile Lindl. produced in Chishui, Zunyi, Guizhou has become a geographical indication product. Dendrobium nobile is used as medicine with stems, sweet, slightly cold in nature, nourishing the stomach and promoting body fluid, nourishing yin and clearing away heat. It is used for yin deficiency and body fluid deficiency, dry mouth, polydipsia, retching due to lack of food, asthenic fever and blurred vision after illness. Studies have shown that Dendrobium plants have anti-tumor, enhanced immunity, anti-oxidation, anti-platelet ...

Claims

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Application Information

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IPC IPC(8): C07D307/86A61P35/00
CPCA61P35/00C07D307/86
Inventor 肖世基尚志梅李晓飞张茂生聂绪强陈永正何芋歧张建永
Owner ZUNYI MEDICAL UNIVERSITY
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