Enrichment method of denitrifying bacteria for development of anticorrosion fungicide for oilfield reinjection water
A technology for oil field re-injection water and denitrifying bacteria, which is applied in the field of denitrifying bacteria enrichment developed by oil field re-injection water antibacterial agents, can solve the problems of dosing, lack of electron donors, and slow formation of obligate denitrifying bacteria groups. , to achieve the effect of reducing costs, rapid oxygen consumption, and maintaining domestication efficiency
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Embodiment 1
[0024] The bio-electrocatalytic domestication device of the present invention is a three-electrode system, including a lower cathode, a middle anode, and an upper cathode in three parts, as follows:
[0025]The anode of this embodiment adopts a carbon brush, the size of the carbon brush is φ50mm, the length is 50mm, the overall length of the carbon brush is 15cm, the carbon fiber is fixed by two titanium wires, and the amount of carbon fiber on each carbon brush is controlled by weight. The weight of the carbon fiber on the brush is 4.215g. The anode is placed in the middle of the reactor, and the titanium wire is used as the wire to connect with the titanium wire on the top of the carbon brush. The carbon fiber has a good specific surface area, can enrich a large number of microorganisms, and has good conductivity . The anode area is flanged to other areas.
[0026] The cathode in this embodiment adopts nickel foam as the cathode, and the size of the nickel foam is 0.5 mm in...
Embodiment 2
[0029] The bioelectrochemical domestication device of the present invention operates as follows:
[0030] Start domestication conditions:
[0031] (1) The effective volume of the reactor is 1.8L, and the aspect ratio is 15:1.
[0032] (2) The water intake formula is as follows:
[0033] Acetate concentration 0.8-1.3g / L; 1ml / L vitamin solution and 1ml / L trace element solution, 30-60mM phosphate buffer solution (pH 7.0);
[0034] Main ingredients and concentrations of vitamins: vitamin H 0.1-0.3g / L, folic acid 0.1-0.3g / L, vitamin B6 0.8-1.2g / L, riboflavin 0.3-0.7g / L, vitamin B1 0.3-0.6g / L , Niacin 0.3~0.6g / L, pantothenic acid 0.4~0.6g / L, B-12 0.01~0.03g / L, P-aminobenzoic acid 0.4~0.6g / L, lipoic acid 0.3~0.6g / L.
[0035] Main composition and concentration of trace elements: MgSO 4 2~4g / L, MnSO 4 ·H 2 O 0.3~0.6g / L, NaCl0.8~1.1g / L, FeSO 4 ·7H 2 O 0.08~0.12g / L, CaCl 2 2H 2 O 0.08~0.2g / L, CoCl 2 ·6H 2 O 0.08~0.12g / L, ZnCl 2 0.1~0.15g / L, CuSO 4 ·5H 2 O 0.01~0.02g / L, Al...
Embodiment 3
[0040] The enrichment and domestication operation of the electrode denitrifying bacteria group of the present invention is as follows:
[0041] It operates at room temperature, and the room temperature is 25±5°C. The wastewater used in the experiment process is taken from the reinjection water of Jiangsu Oilfield, and nitrate is added to maintain the concentration of 100-200mg / L. During the experiment, a peristaltic pump was used to control the hydraulic retention time to 0.5 h, and the dissolved oxygen concentration in the influent water was controlled to be around 4-5 mg / L. Control the external voltage 0.9V during operation.
[0042] The anode carbon brush needs to be pretreated before use. Soak the new carbon brush in acetone for 24 hours, take it out and put it in a muffle furnace for 30 minutes at 450°C, then take it out and cool it to room temperature. Nickel foam can be directly cut according to the size in the embodiment without pretreatment, and then used.
[0043] ...
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