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Bacillus subtilis and application thereof

A technology of Bacillus subtilis and strains, which is applied in the field of biological control of plant diseases, can solve the problems of long breeding cycle of disease-resistant varieties, single resistance, and reduced control effect, and achieve good application prospects, high-efficiency control, and long-diameter effects

Inactive Publication Date: 2019-01-08
宁国市百立德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The breeding cycle of disease-resistant varieties is long and the resistance is single. However, chemical control can easily cause pesticide residues and environmental pollution. At the same time, the long-term use of chemical pesticides makes pathogenic bacteria resistant to them, resulting in decreased control efficiency or even failure.

Method used

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  • Bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Isolation and purification of Bacillus subtilis strain Yt1004

[0022] The Bacillus subtilis strain Yt1004 of the present invention is obtained from the soil by the dilution plate method and the plate streaking method, and the separation method is:

[0023] ⑴Separation of Bacillus: The soil samples were collected in the nursery of Shandong Academy of Forestry, using the 5-point sampling method to collect appropriate amounts of soil around the plot and the central depth in the range of 10-20cm, and mix them evenly. Indicate the location, time and person of collection. Weigh 1g soil sample in 100mL sterile water, place it in a shaker at 30℃, shake at 150rpm for 10min, then incubate it in a water bath at 60℃ for 30min, take 100μL of 10-2, 10-3, 10-4 dilution for coating On the LB medium plate, each gradient was coated in three parallels. After culturing at 30°C for 2 days, the microbial colonies of different forms on the LB medium were picked and streaked on the LB medium p...

Embodiment 2

[0038] 1. Fermentation process of Bacillus subtilis strain Yt1004

[0039] LB liquid medium: The formula is: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, pH adjusted to 7.1-7.4.

[0040] A large amount of solid fermentation medium formula (quality percentage):

[0041] Solid material: 75% corn flour, 10% soybean meal, 15% bran;

[0042] Inorganic salt solution: 3.5% potassium dihydrogen phosphate, 0.04% magnesium sulfate, 4% ammonium sulfate, the rest is water;

[0043] The solid-liquid ratio is 1:1.8 (mass ratio).

[0044] B. subtilis Yt1004 mass solid fermentation process:

[0045] (1) Bacterial seed liquid culture: pick a small amount of Bacillus subtilis strain Yt1004 from the slope of the test tube, transfer to LB liquid medium, shake culture at 30°C for 3 to 5 days, this is the seed liquid;

[0046] (2) Cultivation of solid-producing strains: inoculate the seed solution into a solid medium (500 mL Erlenmeyer flask) at a ratio of 10%, and cultivate at a...

Embodiment 3

[0049] Control Test of Cucumber Root-knot Nematode Disease

[0050] This example provides related experiments on the control effect of the original powder of Bacillus subtilis strain Yt1004 on cucumber root knot nematode disease.

[0051] (1) Test medicine

[0052] Bacillus subtilis strain Yt1004 original powder, prepared in Example 2;

[0053] (2) Test crops and control objects

[0054] Test crop: cucumber; variety: Zhongnong No. 8; control object: cucumber root knot nematode disease;

[0055] (3) Test process

[0056] Test site: Anzhuang Town, Feicheng City, Shandong Province.

[0057] In this experiment, the original powder of Bacillus subtilis strain Yt1004 was diluted 200 times, 400 times, 600 times, and 800 times, and 5 treatments were used as the control. Root irrigation was used on June 5, 2014, with a plot area of ​​50 m2, repeated 4 times. The test agent was mixed with water 1,000 times, the sprayer nozzle was unscrewed, and the cucumber plants were sprayed with 0.5kg of liquid...

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Abstract

The invention discloses bacillus subtilis and an application thereof, and relates to the field of biological preventing and treating plant diseases. The bacterial strain is bacillus subtilis (Bacillussubtilis) Yt1004, and the preservation number is CGMCC No.14672. The invention further discloses a biological preventing and treating preparation containing the bacterial strain and a preparation method of the biological preventing and treating preparation. The microbiological preparation can be used for preventing and treating root-knot nematode diseases including one or more of root-knot nematode diseases of cucumbers, root-knot nematode diseases of tomatoes, root-knot nematode diseases of ginger, root-knot nematode diseases of muskmelons and the like of crops as a biological pesticide or abiological fertilizer. The bacillus subtilis bacterial strain Yt1004 has the characteristics that the bacillus subtilis bacterial strain Yt1004 has long diameter, resists hypoxia, is broad in actionspectrum and high in adaptability, and the bacillus subtilis bacterial strain Yt1004 can rapidly grow and propagate under low nutrients, so that the bacillus subtilis has favorable application prospect. The biological preventing and treating preparation prepared from the bacterial strain can not only effectively prevent and treat root-knot nematode diseases of plants, but also effectively increasethe crop yield, so that the biological preventing and treating preparation has good application prospects.

Description

Technical field [0001] The invention relates to the field of biological control of plant diseases, in particular to a Bacillus subtilis strain and its application. Background technique [0002] Plant disease is a phenomenon in which plants undergo a series of morphological, physiological and biochemical pathological changes under the influence of biological or non-biological factors, which hinder the normal growth and development process, thereby affecting human economic benefits. At present, for the prevention and control of plant diseases, conventional methods include traditional chemical control, breeding of disease-resistant varieties, and biological control. Disease-resistant varieties have a long breeding period and single resistance, and chemical control can easily cause pesticide residues and environmental pollution; at the same time, the long-term use of chemical pesticides makes pathogens resistant to them, resulting in reduced control effectiveness or even failure. Th...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P5/00C12R1/125
CPCA01N63/00C12N1/20C12N1/205C12R2001/125
Inventor 牛兆颖牛赡光张俊生张淑静
Owner 宁国市百立德生物科技有限公司
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