Strain of pseudomonas stutzeri and application thereof

A technology of Pseudomonas stutzeri and Gram-positive bacteria, applied in the field of Pseudomonas stutzeri, can solve the problems of long cycle of disease-resistant varieties, single resistance, pesticide residues, etc., and achieve good application prospects, growth The effect of fast speed and strong resistance

Active Publication Date: 2019-01-15
宁国市百立德生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease-resistant variety has a long cycle and a single resistance
The use of chemicals for chemical control takes a long time and a large amount of use, resulting in pesticide residues and environmental pollution; at the same time, the long-term use of chemical pesticides makes pathogenic bacteria resistant to them, resulting in decreased control efficiency or even failure

Method used

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  • Strain of pseudomonas stutzeri and application thereof
  • Strain of pseudomonas stutzeri and application thereof
  • Strain of pseudomonas stutzeri and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Isolation and purification of Pseudomonas stutzeri strain YT2003

[0023] Pseudomonas stutzeri bacterial strain YT2003 of the present invention adopts dilution plate method and plate streaking method to separate and obtain from soil, and separation method is:

[0024] (1) Separation of bacilli: the collection of soil samples, select plots planted with different types of crops in different regions of the country, and adopt the 5-point sampling method to collect the appropriate amount of soil around and in the center of the plot within a depth of 10-20 cm. Mix in equal amounts. Indicate the location, time and person of the collection. Weigh 1g of soil sample into 100mL sterile water, place it in a shaker at 30°C at 150rpm for 10min, then place it in a water bath at 60°C for 30min, take 100μL of 10-2, 10-3, 10-4 dilution to coat On the LB medium plate, each gradient was coated in three parallels, and after culturing at 30°C for 2 days, microbial colonies of different ...

Embodiment 2

[0036] Fermentation process of Pseudomonas stutzeri strain YT2003

[0037] LB liquid medium: formula: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, pH adjusted to 7.0-7.2.

[0038] A large amount of fermentation medium formula (mass percentage content): 3.0% of soybean flour, 3.0% of corn flour, 0.2% of light calcium carbonate, 0.1% of sodium chloride, the rest is water, pH 7.3.

[0039] Mass fermentation process of Pseudomonas stutzeri strain YT2003:

[0040] ① Strain seed liquid culture: Pick a small amount of bacteria of Pseudomonas stutzeri strain YT2003 from the slant of the test tube, transfer it to LB liquid medium, shake and culture at 30°C for 3-5 days, and this is the seed liquid;

[0041] ②Massive solid fermentation: inoculate the seed solution in a large-scale fermentation medium at a ratio of 1:90. The inoculated raw materials are placed in a fermentation room (at 30° C. and a relative humidity above 85%) for fermentation and culti...

Embodiment 3

[0043] field efficacy test

[0044] This example provides related experiments on the control effect of the original powder of Pseudomonas stutzeri strain YT2003 on watermelon wilt.

[0045] 1) Test drug

[0046] Pseudomonas stutzeri strain YT2003 raw powder (prepared in Example 2); 70% thiophanate-methyl wettable powder (commercially available).

[0047] 2) Test crops and control objects:

[0048] The test crop is watermelon; the variety is Qilin 8424;

[0049] Control object: Fusarium wilt.

[0050] 3) Test site conditions, test design and arrangement

[0051] The test site is located in the watermelon field of Xinhu Village, Wanglao Township, Dongying City, Shandong Province. The soil is clay soil, and the occurrence of Fusarium wilt was heavy in previous years. The cultivation conditions in the experimental field were uniform.

[0052] In this test facility, the ratio of the original powder of Pseudomonas spp. YT2003 was 1:200, 1:400, 1:600; 70% thiophanate-methyl wet...

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Abstract

The invention discloses a pseudomonas stutzeri strain and application thereof, which relates to the field of biological control of plant diseases. The strain is pseudomonas stutzeri YT2003, and the accession number is CGMCC No. 14674. The invention also discloses a biological control preparation comprising the Pseudomonas stutzeri strain YT2003 and a preparation method thereof. The strain of Pseudomonas schrenckii of the invention has the characteristics of rapid growth, wide action spectrum, strong stress resistance, rapid and large-scale colonization in the rhizosphere of plants, and the like, and therefore has good application prospects. The biological control preparation prepared by the Pseudomonas schrenckii strain can be used as a biological pesticide or a biological fertilizer for controlling a variety of soil-borne plant bacterial diseases, including watermelon fusarium wilt, etc.

Description

technical field [0001] The invention relates to the field of biological control of plant diseases, in particular to a strain of Pseudomonas stutzeri and its application. Background technique [0002] Plant diseases seriously affect the growth of agricultural and forestry crops, and even cause crop death, resulting in significant economic losses. For the prevention and control of plant diseases, conventional methods include traditional chemical control, breeding of disease-resistant varieties, and biological control. The disease-resistant variety has a long cycle and a single resistance. Chemical control uses a long time and a large amount of pesticides, resulting in pesticide residues and environmental pollution. At the same time, the long-term use of chemical pesticides makes pathogenic bacteria resistant to them, resulting in reduced control efficiency or even failure. The use of beneficial microorganisms to control plant diseases has broad prospects. [0003] Pseudomon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/38
CPCA01N63/10C12N1/205C12R2001/38
Inventor 牛兆颖牛赡光张俊生张淑静
Owner 宁国市百立德生物科技有限公司
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