A fingerprint of Ganoderma lucidum spore oil and its construction method and application

Abstract

The invention discloses a method for constructing a fingerprint of Ganoderma lucidum spore oil, which comprises the following steps: (1) Preparation of test solution: take Ganoderma lucidum spore oil, add petroleum ether to dissolve, elute with extraction column, add solvent to dissolve after concentrating (2) preparation of reference substance solution: get reference substance Ganoderma lucidum acid A, Ganoderma lucidum acid B, respectively add solvent to dissolve and obtain reference substance stock solution, mix two kinds of reference substance stock solutions with a certain volume to obtain reference substance mixed solution Take ergosterol, add solvent to dissolve; (3) Preparation of reference solution: take 75% ethanol extract of Ganoderma lucidum reference medicinal material, add solvent to dilute; take Ganoderma lucidum spore oil, carry out saponification, extract unsaponifiable matter, and add the obtained residue to dissolve in solvent (4) HPLC detection, formulate the fingerprint of Ganoderma lucidum spore oil. The invention constructs the HPLC fingerprints of the ganoderma lucidum triterpenes and sterol components in the ganoderma lucidum spore oil for the first time, can effectively identify the authenticity of the ganoderma lucidum spore oil, and provides a rapid standard detection method for evaluating the quality of the ganoderma lucidum spore oil.

Description

technical field [0001] The invention relates to a fingerprint of ganoderma lucidum spore oil and its construction method and application, belonging to the technical field of traditional Chinese medicine detection. Background technique [0002] Ganoderma lucidum spore oil is an oil extract made from the spores of Ganoderma lucidum (Leyss.ex Fr.) Karst., a polyporaceae fungus, separated by wall breaking and supercritical carbon dioxide extraction. Ganoderma lucidum spores are extremely fine particles ejected from the cap during the growth and maturity of Ganoderma lucidum, which are the reproductive cells of Ganoderma lucidum. At present, the extraction of Ganoderma lucidum spore oil is generally recognized as supercritical carbon dioxide fluid technology, and the oily substance made from spores is carried out at room temperature, which can prevent heat-sensitive substances from being destroyed, and completely retain the biological activity of the extract. The product has no ...

AI Technical Summary

Problems solved by technology

[0004] At present, many scholars have studied the identification of the fingerprints of the components contained in Ganoderma lucidum spore oil, only involving fat-soluble triglycerides such as glycerol trioleate, 1,2-dioleic acid-3-palmitic acid-triglyceride, etc. There is no published method for fingerprint identification of Ganoderma lucidum triterpenoids and sterols in Ganoderma lucidum spore oil

However, what has been published in professional literature is mostly the method of fingerprint identification of triterpenoids in Ganoderma lucidum fruiting bodies. Volume 10, No. 29, October, 2010, 77-78.) is consistent with the liquid chromatographic conditions described in the published patent CN101435801A, and no standard or reference substance is used for comparison and characterization, and the rigorousness of the obtained results is not enough; both Both of them are similar to the chromatographic conditions in the disclosed fingerprint identification method of triterpenoid components in Ganoderma lucidum fruiting bodies, and are not suitable for the detection and identification of other components other than lipid components in Ganoderma lucidum spore oil; and in Ganoderma lucidum spore oil The lipid components contained in it greatly increase the difficulty of research on other components (such as Ganoderma lucidum triterpenoids and sterols)

Images