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A microcarrier for increasing the culture density of nitrifying bacteria and its preparation method

A technology of nitrifying bacteria and culture density, applied in the field of environmental microorganisms, can solve the problems of poor sludge properties, complex components, and many refractory pollutants, and achieve the effects of improved structural stability, moderate size, and high specific surface area

Active Publication Date: 2022-04-01
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the phenomenon of illegal discharge in industrial enterprises is common. Due to the characteristics of complex components, many refractory pollutants and high toxicity, the nitrification activity of nitrifying bacteria will be inhibited, resulting in the deterioration of sludge properties and the increase of ammonia nitrogen concentration in effluent. high

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  • A microcarrier for increasing the culture density of nitrifying bacteria and its preparation method
  • A microcarrier for increasing the culture density of nitrifying bacteria and its preparation method
  • A microcarrier for increasing the culture density of nitrifying bacteria and its preparation method

Examples

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Effect test

Embodiment 1

[0015] The prepared microcarrier for improving the culture density of nitrifying bacteria in the present embodiment is a calcium carbonate microcarrier, and the specific preparation method is as follows: prepare 0.1mol / L Na 2 CO 3 solution, 0.1mol / L CaCl 2 Each solution was 200mL, and 25mL of 0.5g / L PAA solution was added to Na 2 CO 3 solution and CaCl 2 In the solution, stir at low speed for 0.5h. Add the SDS solution only to the Na 2 CO 3 In the flask of the mixed solution of PAA and SDS, the final concentration of SDS was 10 mM. Adjust the speed to 200r / min, put CaCl 2 The mixed solution with PAA was quickly poured into the three-necked flask, and the reaction was maintained for 1 hour at 50° C. and a rotational speed of 200 r / min. The resulting CaCO 3 The product was filtered, washed twice with deionized water and absolute ethanol, and dried in a vacuum oven at 80°C for 24 hours to obtain CaCO 3 Microcarriers.

[0016] The microcarriers prepared in this example ...

Embodiment 2

[0025] The microcarriers prepared in this example to improve the culture density of nitrifying bacteria are calcium carbonate and basic magnesium carbonate mixed crystal microcarriers. The specific preparation method is as follows: prepare 0.1mol / L of Na 2 CO 3 Solution 200mL, 0.05mol / L CaCl 2 and MgCl 2 Each solution 100mL, CaCl 2 and MgCl 2 The solutions were mixed, and 25 mL of 0.5 g / L PAA solution was added to Na 2 CO 3 solution and CaCl 2 mixed MgCl 2 In the solution, stir at low speed for 0.5h. Add the SDS solution only to the Na 2 CO 3 In the flask of the mixed solution of PAA and SDS, the final concentration of SDS was 20 mM. Adjust the speed to 200r / min, put CaCl 2 The mixed solution with PAA was quickly poured into the three-necked flask, and the reaction was maintained for 1 hour at 50° C. and a rotational speed of 200 r / min. The resulting precipitated product was filtered, washed twice with deionized water and absolute ethanol, and dried in a vacuum oven ...

Embodiment 3

[0030] The microcarriers prepared in this example to improve the culture density of nitrifying bacteria are calcium carbonate and basic magnesium carbonate mixed crystal microcarriers. The specific preparation method is as follows: prepare 0.1mol / L of Na 2 CO 3 Solution 200mL, 0.02mol / L CaCl 2 and 0.08mol / L MgCl 2 Each solution 100mL, CaCl 2 and MgCl 2 Mix the solution, add 25mL of 1.5g / L PAA solution to Na 2 CO 3 solution and CaCl 2 +MgCl 2 In the mixed solution, stir at low speed for 0.5h. Add the SDS solution only to the Na 2 CO 3 In the flask of the mixed solution with PAA, the final concentration of SDS was 15 mM. Adjust the speed to 200r / min, put CaCl 2 The mixed solution with PAA was quickly poured into the three-necked flask, and the reaction was maintained for 1 hour at 70° C. and a rotation speed of 200 r / min. The resulting precipitated product was filtered, washed twice with deionized water and absolute ethanol, and dried in a vacuum oven at 80° C. for 2...

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Abstract

The invention provides a kind of microcarrier that improves nitrifying bacteria culture density, adopts following method to prepare: (1), configure Na respectively 2 CO 3 solution, CaCl 2 solution and MgCl 2 solution, the CaCl 2 solution and MgCl 2 Solution mixing; (2), in Na 2 CO 3 Add polyacrylic acid solution into the solution and stir to obtain mixed solution A, in CaCl 2 and MgCl 2 Add polyacrylic acid solution to the mixed solution and stir to obtain mixed solution B; (3), add sodium lauryl sulfate solution to mixed solution A to prepare mixed solution C, and then pour mixed solution B into mixed solution C The reaction is carried out, and a precipitate is generated after the reaction is completed; (4), the reaction product is filtered and washed, and then vacuum-dried to obtain calcium carbonate and basic magnesium carbonate mixed crystal microcarrier. The above-mentioned microcarrier can increase the culture density of the nitrifying bacteria, and improve the economy of expanding the culture of the nitrifying bacteria.

Description

technical field [0001] The invention belongs to the field of environmental microorganisms, and in particular relates to a method for preparing immobilized microcarriers for increasing the culture density of aerobic nitrifying bacteria and nitrosifying bacteria. Background technique [0002] Nitrifying bacteria are a kind of chemoautotrophic bacteria with nitrifying effect, including two physiological flora of nitrite bacteria (AOB) and nitrate bacteria (NOB). Hour. Sensitive to pH, light, dissolved oxygen, water temperature, and toxic substances. The content in the activated sludge of the common sewage treatment system is low, but it plays a vital role in the denitrification process. In the denitrification process, denitrification and denitrification cannot be performed without nitrification. Therefore, the strength of nitrification ability is directly related to Whether the urban sewage plant can operate normally and whether the effluent can reach the standard. At presen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N11/14C12N1/20C12R1/01
CPCC12N1/20C12N11/14
Inventor 梁晓声
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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