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Method for preparing OPO structure lipid through enzyme catalysis under supercritical CO2 condition

A catalytic preparation and supercritical technology, applied in the field of enzyme catalysis, can solve the problems of low transesterification efficiency and achieve high raw material utilization, low environmental pollution and high selectivity

Active Publication Date: 2019-01-22
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The purpose of the present invention is to overcome the technical defects existing in the prior art, such as: a large amount of organic solvents are used, the transesterification efficiency is low etc., the present invention provides a kind of supercritical CO 2 Enzyme-catalyzed method for preparing OPO structural lipid under condition

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] (1) lipase is modified:

[0030] Dissolve 10mmol of bromopropionic acid in 10mL of acetonitrile and add it to a dry round-bottomed flask. After the dissolution is complete, slowly add 1.1mmol of 1,2-dimethylimidazole dropwise, reflux at 60°C for 5 hours with magnetic stirring, and the reaction is complete. Afterwards, extract twice with anhydrous ether (5*10mL), remove the yellow oily substance in the lower layer, and dry it in vacuum at 70°C for 24 hours to obtain a yellow thick liquid 1,2-methyl 3-propionyl imidazolium bromide (modifier) ;

[0031] Take 1 mmol of 1,2-methyl-3-propionyl imidazolium bromide, add 1 mmol of carbonyldiimidazole, stir magnetically under the action of 2 mL of anhydrous dimethyl sulfoxide, and stop after 2 hours at room temperature. After the reaction is completed, The active body was obtained; 90 μL of the active body was added to 5 mL of Candida rugosa lipase solution with a concentration of 150 μM, and the reaction was stopped after 8 hou...

Embodiment 2

[0037] (1) lipase is modified:

[0038] Dissolve 10mmol of bromopropionic acid in 10mL of acetonitrile and add it to a dry round bottom flask. After the dissolution is complete, slowly add 1.1mmol of 1,butyl-2-methylimidazole dropwise, and reflux at 80°C for 7 hours with magnetic stirring After the reaction, extract twice with anhydrous ether (5*10mL), take off the yellow oily substance in the lower layer, and dry it in vacuum at 70°C for 24h to obtain a yellow thick liquid 1,butyl-2,methyl 3-propionyl imidazole Bromide (modifier);

[0039] Take 1mmol of 1,butyl-2,methyl 3-propionyl imidazolium bromide, add 1mmol of carbonyldiimidazole, stir magnetically under the action of 2mL of anhydrous dimethyl sulfoxide, react at room temperature for 6h and stop, the reaction is complete Afterwards, the active body was obtained; 90 μL of the active body was added to 5 mL of Candida antarctica lipase solution with a concentration of 200 μM, and the reaction was stopped after 12 hours of ...

Embodiment 3

[0045] (1) lipase is modified:

[0046] Dissolve 10mmol of chloroacetic acid in 10mL of acetonitrile and add it into a dry round-bottomed flask. After the dissolution is complete, slowly add 1.1mmol of 1,butyl-2 methylimidazole dropwise, and reflux at 100°C for 10 hours with magnetic stirring. After the end, extract twice with anhydrous ether (5*10mL), remove the yellow oily substance in the lower layer, and dry it in vacuum at 70°C for 24h to obtain a yellow thick liquid 1,butyl-2,methyl 3-acetoxyimidazolium bromide ( Modifier);

[0047] Take 1mmol of 1,butyl-2,methyl 3-acetoxyimidazolium bromide, add 1mmol of carbonyldiimidazole, stir magnetically under the action of 2mL of anhydrous dimethyl sulfoxide, react at room temperature for 8h and stop, after the reaction is completed , to obtain the active body; 90 μL of the active body was added to 5 mL of porcine pancreatic lipase solution with a concentration of 350 μM, and the reaction was stopped after 24 hours of magnetic st...

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PUM

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Abstract

The invention belongs to the field of enzyme catalysis, and in particular relates to a method for preparing OPO structure lipid through enzyme catalysis under the supercritical CO2 condition. The method for preparing the OPO structure lipid through immobilized enzyme catalysis specifically comprises the following steps: taking halogenated carboxylic acid, imidazole compounds, carbonyldiimidazole and a lipase solution as raw materials, and carrying out reaction to obtain an acylated lipase solution; adding zinc nitrate into deionized water; dissolving 2-methylimidazole, the acylated lipase solution and polyvinylpyrrolidone in the deionized water; mixing the solutions, thus obtaining immobilized lipase; and taking glycerol tripalmitate and oleic acid as reaction substrates, adding the immobilized lipase, placing in a reaction still, introducing medium CO2, carrying out reaction, and carrying out separating, thus obtaining the OPO structure lipid. For the method, the reaction conditions are mild, the operation is simple, the utilization rate of the raw materials is high, the conversion rate is high, the purity is high, meanwhile, an enzyme catalyst can be repeatedly utilized, the selectivity is high, the pollution to the environment is small, the production cost is reduced, and the method is expected to realize industrial production.

Description

technical field [0001] The invention belongs to the field of enzyme catalysis, in particular to a supercritical CO 2 A method for preparing OPO structural lipids by enzymatic catalysis under conditions. Background technique [0002] With the development of the economy and the improvement of living standards, people's consumption of food for infants and young children has become higher and higher. Infants and young children are small and their physiological functions are immature. However, in order to achieve normal growth and development, the calorie requirement per kilogram of body weight is more than twice that of adults. If carbohydrates and protein are mainly used to supply energy, each gram can only supply 16KJ of calories, so the amount of food must be quite large, which is not a small burden for babies whose digestive function is not very perfect; while fat can supply more than twice the calories of protein or sugar, that is, 38KJ of calories per gram, Therefore, fa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N11/08
CPCC12N9/20C12N11/08C12P7/6454C12P7/6472C12Y301/01003Y02P20/54
Inventor 邹彬张黎明夏姣姣储嫣红霍书豪
Owner JIANGSU UNIV
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