Sensor for L-cysteine detection and detection method

A cysteine ​​and sensor technology, applied in the field of chemical sensors, can solve the problems of reducing the concentration of hydroxyl radicals in active species, weakening the activity of catalytic hydrogen peroxide decomposition, and the inability to decolorize and decolorize dyes, and achieve a wide detection range. The effect of low cost and convenient preparation

Inactive Publication Date: 2019-01-29
TIANJIN POLYTECHNIC UNIV
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Problems solved by technology

[0007] Chinese patent CN103163121A discloses a detection method of L-cysteine, which uses the characteristics of L-cysteine, borax and chloranil to react in solution, and measures the absorbance value at 420nm by a spectrophotometer Determination of L-cysteine, easy to operate, high specificity, but no detection limit value can be seen
It has the advantages of simple and convenient operation, short time-consuming, fast and specific, visible to the naked eye, sensitive and economical, etc., but the operation is relatively troublesome
[0009] Lin et al. and Pan et al. published in 2015 and 2017, respectively, based on the adsorption of L-cysteine ​​on the surface of platinum-containing composite nanoparticles, reducing the catalytic active sites on the surface to weaken the decomposition activity of catalytic hydrogen peroxide. In turn, the concentration of hydroxyl radicals, the active species that causes the dye to decolorize and decolorize, is reduced, so the dye cannot be decolorized or the decolorization rate is reduced. The colorimetric analysis method for L-cysteine, see Xiao-Qing Lin Hao- HuaDeng, Gang-Wei Wu, Hua-Ping Peng, Ai-Lin Liu, Xin-Hua Lin, Xing-Hua Xiad and WeiChen, Platinum nanoparticles / graphene-oxide hybrid with excellent peroxidase-like activity and its application for cysteine ​​detection, Analyst ,2015,140,5251–5256.N.Pan,L.Wang,L.Wu,C.Peng,Z.Xie,Colorimetric determination of cysteine ​​by exploiting its inhibitory action on the peroxidase-like activity of Au@Pt core-shell nanohybrids , Microchim Acta (2017) 184:65–72, the main problem with this method is the need to use precious metals

Method used

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Examples

Experimental program
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Embodiment 1

[0040] 0.5g Ba with an average particle size of 200nm calcined at 900℃ 0.5 Sr 0.5 co 0.75 Fe 0.2 Ag 0.05 o 3 (Ag-BSCF) ceramic powder was dispersed in deionized water, and then transferred to a 1L volumetric flask to obtain a suspension with a concentration of 0.5g / L for determining the L-cysteine ​​content.

[0041] Preparation of L-cysteine ​​stock solution: Dissolve 0.485 g of L-cysteine ​​in deionized water, and then use a 1 L volumetric flask to obtain a 4 mM L-cysteine ​​stock solution.

[0042] Preparation of L-cysteine ​​standard solution: Take 25mL and 2.5mL of 4mM L-cysteine ​​stock solution respectively and put them into a 100mL volumetric flask to obtain 1mM and 0.1mM L-cysteine ​​standard solution. Then use the same method to prepare the two standard solutions into L-cysteine ​​standard solutions with various concentrations ranging from 0.01 to 400 μM.

[0043] Preparation of other solutions: Dissolve 20mg of methylene blue and 30.738g of PMS in deionized wa...

Embodiment 2

[0049] Prepare the Ag-BSCF suspension of 0.5g / L by the method of embodiment 1.

[0050] The preparation methods of L-cysteine ​​stock solution and standard solution and other solutions are the same as in Example 1. Dissolve 20mg of rhodamine 6G in deionized water, and then use a 1L volumetric flask to obtain a 20ppm rhodamine 6G solution for later use.

[0051] Preparation of detection solution: 50 μL of Ag-BSCF suspension with a concentration of 0.5 g / L, 50 μL of L-cysteine ​​solution at various concentrations (0.01 μM-400 μM), 600 μL of 20 ppm rhodamine 6G solution and 10 μL (50 mM ) PMS solution was shaken and mixed at room temperature and aged for 3 minutes, and then centrifuged at 8000 rpm for 1 minute to obtain a mixed solution for detection.

[0052] Draw a working curve and determine the content of L-cysteine: transfer 200 μL of the above detection solution to a 96-well plate, and measure the absorbance at a wavelength of 529 nm with the solution without L-cysteine ​​...

Embodiment 3

[0056] 0.5g of La with an average particle size of 150nm calcined at 850°C 0.6 Sr 0.4 co 0.2 Fe 0.75 Ag 0.05 o 3 (Ag-LSCF) ceramic powder was dispersed in deionized water, and then transferred to a 1L volumetric flask to obtain a suspension with a concentration of 0.5g / L for determining the L-cysteine ​​content.

[0057] Preparation of L-cysteine ​​stock solution: Dissolve 0.485 g of L-cysteine ​​in deionized water, and then use a 1 L volumetric flask to obtain a 4 mM L-cysteine ​​stock solution.

[0058] Preparation of L-cysteine ​​standard solution: Take 25mL and 2.5mL of 4mM L-cysteine ​​stock solution respectively and put them into a 100mL volumetric flask to obtain 1mM and 0.1mM L-cysteine ​​standard solution. Then use the same method to prepare the two standard solutions into L-cysteine ​​standard solutions with various concentrations ranging from 0.01 to 400 μM.

[0059] Preparation of other solutions: Dissolve 20mg of methylene blue and 24.60g of PMS in deionized...

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Abstract

The invention relates to a chemical sensor in the field of analytical chemistry and specifically relates to a sensor for L-cysteine detection and a detection method. The sensor comprises suspension liquid of calcined perovskite oxide nano-powder, dye solution and peroxysulfate solution. The dye solution is methylene blue solution or rhodamine 6G solution. The sensor is convenient for preparation and cheap in price. Surfaces of nanoparticles do not need to be modified. The invention also provides the detection method. Through utilization of inducing and shielding effects of the L-cysteine for peroxidase-like activity of perovskite nanoparticles, die decoloring activation performance of the perovskite nanoparticles is reduced. A linear relationship between die absorbance and L-cysteine concentration is established. High sensitivity detection for the L-cysteine is realized. Detection is carried out at normal temperature. A pH value of the solution does not need to be controlled. Detectionis convenient and rapid. Cost is low. Detection sensitivity is high. A detection linear range is wide and is 56nM-56muM.

Description

technical field [0001] The invention relates to a chemical sensor in the field of analytical chemistry, in particular to a sensor and a detection method for L-cysteine ​​detection. Background technique [0002] A sensor is an integrated analysis device that selectively recognizes and measures a certain substance or a certain type of substance, that is, a device that converts the chemical information of the measurement object into an analysis signal. It has the advantages of selective identification, fast response, sensitivity, simple instrumentation and operation. [0003] L-cysteine ​​is an amino acid with physiological functions. It is the only amino acid with a reducing group sulfhydryl (-SH) among more than 20 amino acids that make up proteins. Its content and structural distortion are related to many diseases. Such as Alzheimer's disease, liver injury, skin injury and psoriasis, etc., therefore, the quantitative research of L-cysteine ​​in biological and pharmaceutical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31
CPCG01N21/31
Inventor 谭小耀刘利红王少彬朱中华刘少敏
Owner TIANJIN POLYTECHNIC UNIV
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