Carboxyl magnetic microspheres, preparation method and application

A technology of magnetic microspheres and carboxyl groups, which is applied in the preparation of microspheres and microcapsule preparations, can solve the problems of insufficient tightness of the polymer layer, insufficient acid and alkali resistance, and cumbersome experimental operations, and achieve good monodispersity and high Good stability and batch repeatability

Pending Publication Date: 2019-02-15
HANGZHOU JOINSTAR BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has many steps before the in-situ reaction, and the experimental operation is cumbersome; at the same time, in the final sealing process, the polymer layer coated by the swelling polymerization method is not tight enough, and the acid and alkali resistance is not good enough, so it limits its industrialization. Aspects of application

Method used

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  • Carboxyl magnetic microspheres, preparation method and application
  • Carboxyl magnetic microspheres, preparation method and application
  • Carboxyl magnetic microspheres, preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Disperse 2 g of mesoporous microspheres and 2 g of polystyrene-maleic anhydride copolymer (PSMA) in 14 ml of chloroform, and add the PSMA-chloroform solution to the mesoporous microspheres after ultrasonic dispersion for 10 min Continue to ultrasonically disperse for 30 min; then successively wash with absolute ethanol and ultrapure water by centrifugation, and finally store in ultrapure water.

[0050] Weighed 0.696 g of ferric chloride hexahydrate and 0.44 g of ferrous sulfate heptahydrate into 1.5 L of ultrapure water, and then added 2 g of the above PSMA-modified mesoporous microspheres; after ultrasonic dispersion for 30 min, the mixture was added to Into a 5 L reactor, nitrogen gas was introduced, the temperature was raised to 65 °C, and 130 ml of sodium hydroxide solution (0.1 mol / L) was added to continue the reaction for 30 min; after the reaction was completed, it was washed with ultrapure water under centrifugation to obtain magnetic microspheres , the vibrati...

Embodiment 2

[0056] Disperse 2 g of mesoporous microspheres and 2 g of polyacrylic acid (PAA) in 14 ml of chloroform, and after ultrasonic dispersion for 10 min, add the PAA chloroform solution to the mesoporous microspheres and continue ultrasonic dispersion for 30 min; after the reaction Centrifuge and wash with absolute ethanol and ultrapure water in turn, and finally store in ultrapure water.

[0057] Weighed 0.696 g of ferric chloride hexahydrate and 0.44 g of ferrous sulfate heptahydrate into 1.5 L of ultrapure water, and then added 2 g of the above PSMA-modified mesoporous microspheres; after ultrasonic dispersion for 30 min, the mixture was added to Into a 5 L reactor, nitrogen gas was introduced, the temperature was raised to 65°C, and 260 ml of sodium hydroxide solution (0.1 mol / L) was added to continue the reaction for 30 min; after the reaction, it was washed with ultrapure water under centrifugation to obtain magnetic microspheres ;

[0058] Prepare 240 ml of polyethyleneimin...

Embodiment 3

[0063] Take 0.5 mg of magnetic microspheres modified with multiple carboxyl groups (hereinafter referred to as "magnetic beads") in 200 µl of phosphate buffer (PBT solution) (10mM pH7.40), wash three times, and change the PBT solution every 2 minutes. Then draw 200 µl of the prepared 50 mg / ml N-hydroxysuccinimide (NHS) (diluted in PBT) and place it in a centrifuge tube, add 50 mg / ml EDC·HCl200 µl at the same time, mix well and place in a rotating tube Rotate on the mixer for 15 min (at room temperature); adsorb the magnetic beads on the magnetic stand, remove the supernatant, and wash three times with 200 µl PBT solution. Then 200 µl of PBT solution was added, placed in a 37°C incubator and rotated on a rotary mixer for 2 h; the supernatant was removed by magnetic separation, washed three times with 200 µL of PBST (10 mM pH7.40), and dispersed in 500 µL of PBST. The streptavidin (SA) coupling amount of the magnetic beads is tested by the traditional BCA protein concentration t...

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Abstract

The invention provides carboxyl magnetic microspheres, a preparation method and an application. The surfaces of mesoporous polymer microspheres are modified with functional groups with a physical adsorption method, carboxyl magnetic microspheres are prepared with an in-situ coprecipitation synthesis method, and magnetic microspheres modified with carboxyl repeatedly is prepared by silica coating,amino modification and carboxyl re-modification. The carboxyl repeatedly modified magnetic microspheres are functional composite microspheres, have the advantages of magnetic responsiveness, high stability, better biological compatibility, uniform particle size, good monodispersity, controllable magnetic content and good batch repeatability, are suitable for large-scale stable preparation in industry, and can be widely applied to the fields of cell separation, immune detection, protein purification, immobilized enzyme, environmental protection and the like.

Description

technical field [0001] The invention relates to a carboxyl magnetic microsphere, a preparation method and an application. Background technique [0002] Since the 1970s, magnetic microspheres have developed rapidly as a functional composite microsphere, which has the characteristics of magnetic response performance, good dispersion, and surface functional diversity. Enzyme, protein separation and so on have a wide range of applications. [0003] Chinese patent (CN 103012828 A) discloses a preparation method of polymer iron oxide microspheres, that is, firstly prepare porous polymer microspheres, and then synthesize iron oxide nanoparticles inside the microspheres by co-precipitation method to obtain polymer / iron oxide microspheres Composite microspheres are finally coated with silicon dioxide to obtain magnetic microspheres. However, this method uses surface-functionalized porous polymer microspheres modified by chemical reactions, and the number of functional groups on the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J13/06
CPCB01J13/06
Inventor 刘翔王耀洪明徐宏薛等斌古宏晨何国清王巨锋刘伟周旭一
Owner HANGZHOU JOINSTAR BIOTECH
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