Method for degrading antibiotics in livestock manure

A technology for livestock and poultry manure and antibiotics, applied in the field of environmental pollution remediation, can solve the problems of reduced effect, inability to degrade antibiotics, ignoring the sensitivity of bacterial strains to antibiotics, etc., and achieve the effect of complete degradation

Inactive Publication Date: 2019-02-19
陈先锐
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In the above methods, microorganisms are used to degrade antibiotics, but the potential pathogenic danger caused by microbial strains to the human body or the environment is ignored, and the sensitivity of bacterial strains to antibiotics is ignored. The production of bacterial strains without antibiotic resistance is inhibited by antibiotics, so that they cannot Degrade antibiotics, and there are many kinds of antibiotics in feces. A single strain and method cannot remove all the residues of these antibiotics, and the effect is reduced

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Preparation of resistant strains: Inoculate the primary bacteria of Bacillus pumilus, Sphingomonas pumilus, Aspergillus niger, and Trichoderma viride into the corresponding solid medium respectively by coating method, and Bacillus pumilus and Sphingosine Cultivate single cell bacteria at 37°C for 7 days, Aspergillus niger and Trichoderma viride for 5 days at 25°C; after the cultivation, use a sterile inoculation loop to select the correct strain that grows well from the medium, and use the three-line method to inoculate In the corresponding solid medium containing antibiotics, set the temperature of the incubator at 30°C; when the strain enters the growth phase and grows to the third line, use a sterile inoculation loop to select a single colony that grows well in the third line and inoculate it in a new medium containing 0.05% penicillin, 0.01% kanamycin, 0.15% tetracycline, 0.05% erythromycin and 0.02% florfenicol in the corresponding solid medium, at 30 ℃ for puri...

Embodiment 2

[0046] (1) Preparation of resistant strains: Inoculate the primary bacteria of Bacillus pumilus, Sphingomonas pumilus, Aspergillus niger, and Trichoderma viride into the corresponding solid medium respectively by coating method, and Bacillus pumilus and Sphingosine Cultivate single cell bacteria at 37°C for 6 days, Aspergillus niger and Trichoderma viride for 5 days at 25°C; after the cultivation, use a sterile inoculation loop to select the correct strain that grows well from the medium, and use the three-line method to inoculate In the corresponding solid medium containing antibiotics, set the temperature of the incubator at 25°C; when the strain enters the growth phase and grows to the third line, use a sterile inoculation loop to select a single colony that grows well in the third line and inoculate it in a new medium containing 0.05% penicillin, 0.02% kanamycin, 0.15% tetracycline, 0.05% erythromycin and 0.04% florfenicol in the corresponding solid medium, at 30 ℃ for puri...

Embodiment 3

[0053] (1) Preparation of resistant strains: Inoculate the primary bacteria of Bacillus pumilus, Sphingomonas pumilus, Aspergillus niger, and Trichoderma viride into the corresponding solid medium respectively by coating method, and Bacillus pumilus and Sphingosine Cultivate single cell bacteria at 37°C for 7 days, Aspergillus niger and Trichoderma viride for 5 days at 25°C; after the cultivation, use a sterile inoculation loop to select the correct strain that grows well from the medium, and use the three-line method to inoculate In the corresponding solid medium containing antibiotics, set the temperature of the incubator at 30°C; when the strain enters the growth phase and grows to the third line, use a sterile inoculation loop to select a single colony that grows well in the third line and inoculate it in a new medium containing 0.05% penicillin, 0.03% kanamycin, 0.15% tetracycline, 0.05% erythromycin and 0.06% florfenicol in the corresponding solid medium, at 30 ℃ for puri...

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Abstract

The invention discloses a method for degrading antibiotics in livestock manure. The method includes the steps: firstly, fermenting and degrading the antibiotics in the livestock manure for the first time by resistant bacillus pumilus, sphingomonas xenophaga, Aspergillus niger and trichoderma viride; secondly, further degrading the antibiotics into small molecule substances by the aid of algae decomposing agents and an infrared radiation technology; finally, inactivating microorganisms at high temperature generated by secondary fermentation. According to the method, the antibiotics in the livestock manure can be effectively degraded into small molecule non-toxic substances, worm eggs in the manure can be killed, harm of the antibiotics in the livestock manure to environments is fundamentally avoided, the livestock manure subjected to secondary fermentation can be prepared into organic fertilizers and recycled, and waste is turned into wealth.

Description

technical field [0001] The invention belongs to the technical field of environmental pollution restoration, and in particular relates to a method for degrading antibiotics in livestock and poultry manure. Background technique [0002] With the vigorous development of intensive farming, veterinary antibiotics have become an indispensable part of modern agriculture and breeding. However, antibiotics cannot be completely absorbed through the animal intestines, and most of them will be excreted in feces and urine in the form of prototypes or metabolites, resulting in a large accumulation of antibiotics in livestock and poultry feces. At present, antibiotics widely used in livestock and poultry farming include quinolones, polypeptides, tetracyclines, macrolides, sulfonamides, and aminoglycosides. Due to the complex structure, difficult biodegradation and good water solubility of these antibiotics, they are easy to store and accumulate in the environment. When these antibiotics ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F11/00C02F11/12C02F11/02C02F11/16C12N1/20C12N1/14C05F15/00C05F17/00C12R1/07C12R1/685C12R1/885
CPCC02F11/00C02F11/02C02F11/12C02F11/16C02F2303/06C05F3/00C05F17/00C12N1/14C12N1/20C05F11/00C05F11/08Y02W30/40
Inventor 陈先锐
Owner 陈先锐
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