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A kind of polydopamine nanodiagnostic agent and preparation method thereof

A polydopamine nanometer and polydopamine technology, applied in the field of biomedical materials, can solve the problems of oxygen consumption, aggravate tumor tissue hypoxia, adverse photodynamic therapy, etc., and achieve the effects of improving hypoxia and improving catalytic activity.

Active Publication Date: 2021-06-25
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with normal tissues, tumors are in a hypoxic environment, which is not conducive to the effective implementation of photodynamic therapy, and the treatment process will consume oxygen, thereby aggravating the hypoxia in tumor tissues.

Method used

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  • A kind of polydopamine nanodiagnostic agent and preparation method thereof
  • A kind of polydopamine nanodiagnostic agent and preparation method thereof
  • A kind of polydopamine nanodiagnostic agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Embodiment 1: Preparation of Rh@MPDA nanoparticles

[0040] (1) Preparation of MPDA nanoparticles:

[0041] The carrier mesoporous polydopamine MPDA is prepared from the reference literature, and the particle size is about 200nm.

[0042] Weigh 0.15g of dopamine hydrochloride and 0.1g of F-127, dissolve them in 10mL of ethanol and ultrapure water 1:1 system, stir for 20min to fully dissolve and mix. In an ultrasonic water bath, slowly add 160 μL of TMB, ultrasonic for 3 minutes, the solution becomes milky white visible to the naked eye. Then add 375 μL of ammonia water, the solution turns dark brown, stir on a magnetic stirrer for 2 hours, and centrifuge for 10 minutes (13000 rpm / min, 4°C) to collect the precipitate. Wash once with ethanol, wash twice with ultrapure water, and finally resuspend with ultrapure water to obtain the mesoporous polydopamine solution.

[0043] (2) Preparation of Rh@MPDA nanoparticles:

[0044] Centrifuge 10 mL of 1 mg / mL mesoporous polydo...

Embodiment 2

[0047] Embodiment 2: Preparation of Rh@MPDA nanoparticles

[0048] (1) Same as Step 1 of Example 1;

[0049] (2) Same as Step 2 of Example 1, except that 1 mL of 10 mg / mL rhodium trichloride hydrate solution was added.

[0050] (3) Transmission electron microscope observation:

[0051] After fully ultrasonically dispersing the Rh@MPDA nanoparticles prepared above, they were added dropwise onto the copper grid, dried overnight in the air at room temperature to collect the copper grid, and observed with a transmission electron microscope. The transmission electron microscope picture is as follows: image 3 shown. As can be seen from the figure, the distribution of rhodium nanoparticles in the above-mentioned Rh@MPDA is relative to that of Example 1 ( figure 2 ) is denser, indicating that increasing the dosage of rhodium precursors can effectively increase the loading of rhodium nanoparticles.

Embodiment 3

[0052] Embodiment 3: Preparation of Rh@MPDA nanoparticles

[0053] (1) Same as Step 1 of Example 1;

[0054] (2) Same as Step 2 of Example 1, except that 2 mL of 10 mg / mL rhodium trichloride hydrate solution was added.

[0055] (3) Transmission electron microscope observation:

[0056] After fully ultrasonically dispersing the Rh@MPDA nanoparticles prepared above, they were added dropwise onto the copper grid, dried overnight in the air at room temperature to collect the copper grid, and observed with a transmission electron microscope. The transmission electron microscope picture is as follows: Figure 4 shown. As can be seen from the figure, the content of rhodium loaded in the above-mentioned Rh@MPDA is relative to that of Example 1 ( figure 2 ) and Example 2 ( image 3 ) are more, and the excess rhodium nanoparticles are aggregated and distributed on the surface of the carrier, which further shows that increasing the dosage of rhodium precursors can effectively increa...

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Abstract

The invention discloses a polydopamine nanometer diagnosis and treatment preparation and a preparation method thereof. The nano-therapeutic agent uses mesoporous polydopamine as a carrier, and carries rhodium nanoparticles in the pores and surfaces of mesoporous polydopamine respectively through hydrothermal synthesis reaction, and then absorbs photosensitizer chlorin (Ce6) on polydopamine and polydopamine. The polydopamine nanometer therapeutic agent with good dispersion is obtained on the composite nanoparticle of rhodium. The invention utilizes the photothermal effect of the carrier mesoporous polydopamine and the rhodium nanoparticle itself to locally heat up the tumor site. Combined with the catalytic properties of rhodium nanoparticles, it catalyzes the decomposition of hydrogen peroxide in the tumor microenvironment to generate oxygen. Under the conditions of photosensitizer Ce6 and external laser, it can realize the generation of singlet oxygen and effectively kill cancer cells. At the same time, combined with the photoacoustic imaging properties of polydopamine, the purpose of photoacoustic imaging-guided photothermal therapy and photodynamic synergistic therapy can be realized, which is expected to improve the effect of tumor treatment and has good biocompatibility, which has the potential for clinical application.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a polydopamine nanometer diagnosis and treatment agent and a preparation method thereof. Background technique [0002] Cancer is a major disease that threatens human life. The current common radiotherapy, chemotherapy and surgery have their own limitations, so that the treatment effect of cancer is not ideal. Therefore, a large number of researchers focus on the integration of diagnosis and treatment, use the advantages of nanostructures to concentrate the functions of diagnosis and treatment into nanoparticles, use the EPR effect to target tumor sites, and realize the diagnosis and treatment of cancer at the same time. [0003] Photothermal therapy technology, as a new type of treatment strategy, has received high attention in the field of cancer diagnosis and treatment in recent years. Photothermal therapy based on nanomaterials uses near-infrared light with strong tissue ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K47/34A61K47/69A61K49/22A61P35/00
CPCA61K41/0052A61K41/0071A61K47/34A61K49/22A61K47/6949A61P35/00A61K2300/00
Inventor 曹众丁梦丽
Owner SUN YAT SEN UNIV
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