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Detection method of L-2-amino-5-guanidinovaleric acid enantiomer

A technology for enantiomers and arginyl valeric acid is applied in the field of detection of L-2-amino-5-arginyl valeric acid enantiomers, and can solve problems such as no relevant reports on the analysis and separation method, and the like, To achieve the effect of ensuring food and medicinal safety, good separation, and ensuring safety

Active Publication Date: 2019-02-26
FUJIAN GUTIAN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no relevant report on the separation method for the analysis and separation of the two enantiomers of 2-amino-5-guanidinovaleric acid

Method used

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  • Detection method of L-2-amino-5-guanidinovaleric acid enantiomer
  • Detection method of L-2-amino-5-guanidinovaleric acid enantiomer
  • Detection method of L-2-amino-5-guanidinovaleric acid enantiomer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1: Detection of enantiomers of three batches of L-2-amino-5-guanidinovaleric acid bulk drug

[0051] Instrument and chromatographic conditions:

[0052] Agilent 1260 high performance liquid chromatography was used, with octadecylsilane bonded silica gel as filler (4.6×250mm, 5μm), column temperature was 25°C; flow rate was 1.0mL / min; detection wavelength was 250nm; injection volume Take 1.36 grams of potassium dihydrogen phosphate, add water to dissolve and dilute to 1000mL, use 20% phosphoric acid to adjust the pH value to 2.8 as mobile phase A, use methanol as mobile phase B, and perform elution in the following table, and the ratio is shown in the table 1 shows:

[0053] Table 1

[0054]

[0055]

[0056] Precisely measure 100 μl each of the test solution and the enantiomer reference solution, and add an equal volume of derivatization test solution 1 (take 2,3,4,6-tetra-O-acetyl-β-D-pyridine Add an appropriate amount of glucopyranose isothiocyanate (...

Embodiment 2

[0067] Example 2: Enantiomeric Orientation Test

[0068] Instrument and chromatographic conditions are the same as in Example 1.

[0069] Experimental procedure: Weigh an appropriate amount of L-2-amino-5-guanidinovaleric acid and its enantiomer reference substance respectively, dissolve them in diluent and quantitatively dilute them to make a solution containing about 0.5mg per 1mL as a positioning solution The preparation method of the mixed solution is the same as the system suitability solution of Example 1, and then derivatized before the column, and each derivatized solution is injected into the liquid chromatograph respectively, and the chromatogram is recorded. The separation results of the derivative peaks of L-2-amino-5-guanidinovaleric acid enantiomers and adjacent peaks are shown in Table 4, and the chromatograms are shown in Figure 5 :

[0070] Table 4

[0071]

[0072] The results showed that: L-2-amino-5-guanidinovaleric acid enantiomeric derivative peaks...

Embodiment 3

[0073] Embodiment 3: Specific destruction test

[0074] Instrument and chromatographic conditions are the same as in Example 1.

[0075] Experimental procedure: Accurately weigh an appropriate amount of L-2-amino-5-guanidinovaleric acid, and conduct a forced destruction test on it under the conditions of 0.5mol / L hydrochloric acid solution, high temperature of 180°C and strong light of 4500Lx, and the preparation method of each destroyed sample See Table 5, chromatogram see Figure 6 ~ Figure 9 :

[0076] Prepare each specific destruction sample solution in the following table:

[0077] table 5

[0078]

[0079] Take the above-mentioned derivatization solutions and inject samples respectively and record the chromatograms. The destruction results are shown in Table 6:

[0080] Table 6

[0081]

[0082]

[0083] The results showed that no D-2-amino-5-guanidine valeric acid was detected in the samples destroyed by strong acid, high temperature and strong light, indi...

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Abstract

The invention discloses a detection method of an L-2-amino-5-guanidinovaleric acid enantiomer and belongs to the technical field of food and drug analysis. The method mainly comprises the following steps: preparing a system applicable solution; preparing an enantiomer reference solution; preparing a test solution; preparing a derivatizing reagent; performing pre-column derivatization and chromatographic condition and system suitability tests; determining the L-2-amino-5-guanidinovaleric acid enantiomer. The detection method has higher sensitivity, accuracy, precision and durability, can accurately reflect content of the L-2-amino-5-guanidinovaleric acid enantiomer and is applicable to detection and quality control of the L-2-amino-5-guanidinovaleric acid enantiomer in industry, so that reference is provided for setting reasonable quality standards, product quality can be controlled and mastered better, and edible and medicinal safety is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of food and drug analysis, and in particular relates to a method for detecting the enantiomer of L-2-amino-5-guanidinovaleric acid. Background technique [0002] L-2-amino-5-guanidinovaleric acid (CAS registration number 74-79-3) belongs to amino acid compounds, has important physiological functions, is often used as nutritional supplements, and is also an important component of amino acid capsules and infusions. L-2-amino-5-guanidine valeric acid can effectively improve immunity, promote the immune system to secrete endogenous substances, help fight cancer cells and prevent virus infection; help patients with severe trauma and burns that require a large amount of tissue repair health care. Clinically, L-2-amino-5-guanidine valeric acid is suitable for hepatic coma with increased blood ammonia, especially for patients with alkalosis; it is used to assist in the determination of pituitary function; it is use...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/86
CPCG01N30/02G01N30/06G01N30/86G01N2030/067
Inventor 陈程俊李绪全刘红方佳茂陈伟滨林晓群张松浩陈榕兴
Owner FUJIAN GUTIAN PHARMA
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