Preparation process of compound preparation of amino oligosaccharin and bacillus

An amino oligosaccharide and a preparation process technology, which are applied in the field of preparation of Bacillus and amino oligosaccharide compound preparations, can solve the problems of reduced viable bacteria content, short shelf life, etc. The effect of less investment in equipment

Inactive Publication Date: 2019-03-12
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, although Bacillus exists in the form of endospores, and has strong resistance and high stability to adverse environmental factors such as drying, high temperature, high pressure, and oxidation during processing and transportation, Bacillus preparations still commonly exist in processing and transportation. During transportation and during the validity period, the content of viable bacteria is reduced, and the shelf life is short, etc.

Method used

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  • Preparation process of compound preparation of amino oligosaccharin and bacillus

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] (1) Activation of Bacillus

[0037] Inoculate Bacillus cereus and Bacillus licheniformis in their respective slant culture media, culture them at 32°C for 24 hours, and then wash their respective slant surfaces with sterile saline with a mass concentration of 15% to prepare concentrations of 2 ×10 6 cfu / mL bacterial suspension. The slant medium formula of Bacillus cereus is: yeast extract 3.5g / L, soybean peptone 8.5g / L, corn starch 12g / L, NaCl 8g / L, pH value is 7.0; the slant medium formula of Bacillus licheniformis is : Yeast extract 3.5g / L, tryptone 8g / L, agar powder 15g / L, NaCl 3.5g / L, pH value 7.0.

[0038] (2) shake flask culture of bacillus:

[0039]The bacterium suspension of two kinds of bacteria prepared in step (1) is respectively inoculated in respective liquid culture medium and cultivated, and the ratio of bacterium suspension volume and liquid culture medium volume is 10%, cultivates in shake flask under 32 ℃ 24h, the rotating speed of the shaker is 15...

Embodiment 2

[0048] (1) Activation of Bacillus

[0049] Bacillus cereus and Bacillus licheniformis were inoculated in their respective slant culture medium, cultured at 37°C for 20 h, and then the respective slant surfaces were washed with sterilized saline with a mass concentration of 13% to prepare concentrations of 4 ×10 6 cfu / mL bacterial suspension. The slant medium formula of described Bacillus cereus is: yeast extract 5g / L, soybean peptone 10g / L, cornstarch 15g / L, NaCl 10g / L, and the pH value is 7.2; The slant medium formula of Bacillus licheniformis is : Yeast extract 5g / L, tryptone 10g / L, agar powder 20g / L, NaCl 5g / L, pH value is 7.2.

[0050] (2) shake flask culture of bacillus:

[0051] The bacterium suspension of two kinds of bacteria prepared in step (1) is inoculated in respective liquid medium and cultivated, the ratio of bacterium suspension volume and liquid medium volume is 8%, cultivates 20h in shake flask under 37 ℃ , the shaking table speed is 180rpm. The formula ...

Embodiment 3

[0060] (1) Activation of Bacillus

[0061] Bacillus cereus and Bacillus licheniformis were inoculated in their respective slant culture media, cultured at 35°C for 22 hours, and then the respective slant surfaces were washed with sterile saline with a mass concentration of 18% to prepare concentrations of 3 ×10 6 cfu / mL bacterial suspension. The slant medium formula of Bacillus cereus is: yeast extract 3g / L, soybean peptone 7g / L, corn starch 12g / L, NaCl 7g / L, pH value 7.5; the slant medium formula of Bacillus licheniformis is: yeast Cream 3g / L, tryptone 9g / L, agar powder 18g / L, NaCl 3g / L, pH value is 7.5.

[0062] (2) shake flask culture of bacillus:

[0063] The bacterium suspension of two kinds of bacteria prepared in step (1) is inoculated in respective liquid medium and cultivated, the ratio of bacterium suspension volume and liquid medium volume is 13%, cultivate 22h in shake flask under 35 ℃ , the shaking table speed is 130rpm. The formula of the liquid culture medi...

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Abstract

The invention discloses a preparation process of a compound preparation of amino oligosaccharin and bacillus. The process comprises the following steps of obtaining a solid fermentation product by adopting a method of mixed fermentation of bacillus cereus and bacillus licheniformis, mixing the solid fermentation product uniformly with amino oligosaccharin as a plant growth regulator, humic acid and polyglutamic acid, in a certain ratio, and then drying by centrifugal atomization to obtain the compound preparation of the bacillus and the amino oligosaccharide. After applied to crops, the microbial agent not only can play a role in preventing and treating diseases to improve the stress resistance of the crops, but also can have a regulating effect on the growth of plants to greatly increasethe yield of the crops. The production process is simple, the equipment investment is small, and no residual pollution exists, so that the process has a relatively high application value.

Description

technical field [0001] The invention relates to a preparation process of a bacillus and amino-oligosaccharin compound preparation, belonging to the field of biotechnology. technical background [0002] my country is the country with the largest production and use of pesticides. In recent years, food safety problems caused by chemical pesticide residues in my country have become very prominent. Environmental pollution and food safety caused by long-term dependence and extensive use of chemical pesticides have brought many adverse effects on the sustainable development of agriculture and become the biggest problem to be solved urgently in social and economic development. Bacillus is a strain officially approved by the Ministry of Agriculture of my country to produce biological products. This strain has the functions of preventing diseases and promoting plant growth. It has been widely promoted and applied at home and abroad. field. [0003] Bacillus cereus is a kind of Bacill...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/16A01N63/00A01N61/00A01N37/46A01P21/00C12N1/20
CPCA01N37/46A01N43/16A01N61/00A01N63/00C12N1/20Y02A40/10
Inventor 胡永红卜悦意杨文革周翼钱永根章泳应汉杰
Owner NANJING UNIV OF TECH
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