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Probes based on mesoporous carbon nanospheres doped with gold nanoparticles and their preparation

A technology of gold nanoparticles and carbon nanospheres, which is applied in the field of cancer cell diagnosis and treatment probes, can solve the problems of restricted use, difficult to metabolize liver toxicity, etc., to prevent aggregation, improve biocompatibility, and improve targeting. Effect

Active Publication Date: 2021-07-20
SHANGHAI JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common inorganic materials are quantum dots and some other fluorescent dyes, but these molecules are often not easy to metabolize, resulting in certain liver toxicity, which limits their use in vivo

Method used

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  • Probes based on mesoporous carbon nanospheres doped with gold nanoparticles and their preparation
  • Probes based on mesoporous carbon nanospheres doped with gold nanoparticles and their preparation
  • Probes based on mesoporous carbon nanospheres doped with gold nanoparticles and their preparation

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Experimental program
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Effect test

Embodiment 1

[0048] 1. Reagents

[0049] In this example, all the chemical reagents used were of analytical grade or above. Chlorauric acid (HAuCl 4 ·4H2O), formaldehyde (37wt.%) and phenol were purchased from Shanghai Titan Technology Co., Ltd. F127 (Mw=12,600, PEO106PPO70PEO106) was purchased from Acros Company. Hydrochloric acid (HCl, 37%), concentrated sulfuric acid (H 2 SO 4 ,98%), concentrated nitric acid (HNO 3 ,≥97.2%), folic acid (FA), dimethylsulfoxide (DMSO) and sodium borohydride (NaBH 4 ) were purchased from Sinopharm Co., Ltd. N-hydroxysuccinimide (NHS), bovine serum albumin (BSA, 99.9%) and 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) were purchased from Aladdin Ltd. Apoptosis Detection Kit, 4',6-diamidino-2-phenylindole dihydrochloride (DAPI), CCK-8 kit (CCK-8) and Calcein-AM / PI Live / Dead Cell double staining kits were purchased from Yisheng Co., Ltd. Fetal bovine serum albumin (FBS), cell culture medium (DMEM), and trypsin digest were purchase...

Embodiment 2

[0067] Adopt the preparation step identical with embodiment 1, difference is:

[0068] (1) When preparing mesoporous carbon nanospheres doped with gold nanoparticles composites: the mass ratio of phenol, formaldehyde and sodium hydroxide is 0.5:2:5, and the mixing temperature of phenol, formaldehyde and sodium hydroxide is 65°C , the stirring time is 40min; in the aqueous solution of polyether F127 added, the ratio of the mass of polyether F127 to the mass of phenol is 1.5:1, and the temperature when adding the aqueous solution of polyether F127 is 60°C, and the stirring time is 4h ; Added 3-mercaptopropyltrimethoxysilane, HAuCl 4 1. The mass ratio of pure water to phenol is 60 6:20; the reaction temperature is 65°C, and the reaction time is 18h; the hydrothermal reaction temperature is 120°C, and the reaction time is 30h; washing is carried out alternately with ethanol and pure water , the number of washings is more than 3 times; the drying temperature is 50°C, and the dryin...

Embodiment 3

[0075] Adopt the preparation step identical with embodiment 1, difference is:

[0076] (1) When preparing mesoporous carbon nanospheres doped with gold nanoparticles composites: the mass ratio of phenol, formaldehyde and sodium hydroxide is 1:1:10, and the mixing temperature of phenol, formaldehyde and sodium hydroxide is 75°C , the stirring time is 20min; in the aqueous solution of polyether F127 added, the ratio of the mass of polyether F127 to the mass of phenol is 2.5:0.5, and the temperature when adding the aqueous solution of polyether F127 is 70°C, and the stirring time is 2h ; Added 3-mercaptopropyltrimethoxysilane, HAuCl 4 1. The mass ratio of pure water to phenol is 65:4:30; the reaction temperature is 70°C, and the reaction time is 12h; the hydrothermal reaction temperature is 150°C, and the reaction time is 20h; ethanol and pure water are used for washing alternately Washing, the number of washings is more than 3 times; the drying temperature is 80°C, and the dryi...

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Abstract

The invention relates to a probe based on mesoporous carbon nanospheres doped with gold nanoparticle materials and a preparation method thereof. The probe comprises mesoporous carbon nanospheres, gold nanoparticles grown on the inner channel walls of the mesoporous carbon nanospheres, Organic fluorescent substances loaded in the channels of mesoporous carbon nanospheres and targeting molecules connected on the surface of mesoporous carbon nanospheres through chemical bonds. Compared with the prior art, the present invention can not only realize diagnostic monitoring, but also can be used as a therapeutic probe, and has good targeting and excellent therapeutic effect based on PTT and PDT. Finally, the synthesized probes successfully ablated tumors (tumors of MGC-803 cells in tumor-bearing mice) and were subjected to in vivo and in vitro fluorescence imaging. In addition, the gold particles located inside the mesoporous carbon nanospheres are not only well protected, but also fully play the role of nanozyme catalysis.

Description

technical field [0001] The invention relates to the technical field of cancer cell diagnosis and treatment probes, in particular to a probe based on mesoporous nanocarbon spheres doped with gold nanoparticles and its preparation. Background technique [0002] As we all know, cancer is a major disease with high morbidity and mortality in the whole country and even in the world, which poses a great threat to human life and health safety. Cancer is a major killer that threatens human life and health, and it is also a major problem in the medical field. Early diagnosis of cancer is of great significance to the treatment of tumors, and early precision treatment can achieve good therapeutic effects on tumors. The current clinical diagnostic technology is still insufficient, and the early diagnosis effect is not ideal. The lack of early diagnosis methods leads to unsatisfactory diagnosis and treatment of tumor patients, so finding a technology that can accurately diagnose early i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K47/04A61K47/64A61K47/54A61K41/00A61P35/00
CPCA61K41/0052A61K49/0021A61K49/005A61K49/0052A61K49/0056A61P35/00
Inventor 崔大祥章阿敏潘少君常杰黄志成
Owner SHANGHAI JIAOTONG UNIV
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