Streptococcus faecium and application thereof
A technology of Streptococcus faecalis and bacterial sludge, which is applied in the field of human microecology, can solve the problem of low survival rate of Streptococcus faecalis bacteria powder, etc., and achieve the effect of good acid resistance and bile salt resistance, ideal bacterial activity, and promoting intestinal peristalsis.
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[0031] Example 1 Isolation and screening of Streptococcus faecalis
[0032] The streptococcus faecalis with the deposit number of CGMCC No. 16128 of the present invention is isolated from human feces and obtained by the following method:
[0033] Put 1 gram of human intestinal feces into a sterile bottle containing 30 glass balls and add physiological saline to make a 100-fold dilution, and then make 10 -3 , 10 -4 , Sequentially to 10 -8 Dilution degree, take 10 -4 , 10 -5 , 10 -6 , 10 -7 , 10 -8 Five dilutions of bacterial solution were respectively inoculated on LYT Universal Agar Plate, Eosin, Meilan Agar, BB (BS) Bifidobacterium selective medium, LBS (LC) Lactobacillus selective medium, EC enterococcus selective base And Sb yeast selection base. Each medium is connected to 6 plates. Immediately after inoculation, turn the petri dish up and down to spread the inoculum evenly on the surface of the plate. Three of them were cultured aerobic and observed after 24 hours. The other...
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[0049] Example 2 Preparation of freeze-dried powder of Streptococcus faecalis
[0050] The Streptococcus faecalis in this example is a strain with the deposit number CGMCC No. 16128, which is prepared as a freeze-dried powder of Streptococcus faecalis by the following method:
[0051] (1) Take the described Streptococcus faecalis, inoculate it into the primary fermentation medium according to the inoculum of 1%, and then ferment it for 12-14h under the conditions of 37±3℃ and 100-180rpm. First-level fermentation broth; then, take the first-level fermentation broth, inoculate it into the second-level fermentation medium according to the inoculum amount of 5-10%, and cultivate at a temperature of 37±3°C and a rotation speed of 100-180rpm 4-6h to obtain the secondary fermentation broth; then take the secondary fermentation broth and inoculate it into the tertiary fermentation medium at an inoculum of 5-12% at a temperature of 37±3°C and a rotation speed of 150-250rpm Under the condit...
Example Embodiment
[0055] Example 3 Preparation of freeze-dried powder of Streptococcus faecalis
[0056] The Streptococcus faecalis in this example is a strain with the deposit number CGMCC No. 16128, which is prepared as a freeze-dried powder of Streptococcus faecalis by the following method:
[0057] (1) Take the said Streptococcus faecalis and culture it in coagulated milk with an initial pH of 7.5 at a temperature of 37°C for 36 to 48 hours. As a preferred implementation of this embodiment, the said Streptococcus faecalis is 3% The inoculum is inoculated on the coagulated milk as the culture medium, and cultivated by shaking flask culture;
[0058] (2) Take the fermentation broth obtained by fermentation in step (1), place it in a centrifuge at a rotation speed of 8000r / min, centrifuge for 30min, and collect bacterial sludge;
[0059] (3) The protective agent is added to the bacterial mud obtained in step (2), and the protective agent is mixed with sucrose, skimmed milk powder and gelatin in a weig...
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