Streptococcus faecium and application thereof

A technology of Streptococcus faecalis and bacterial sludge, which is applied in the field of human microecology, can solve the problem of low survival rate of Streptococcus faecalis bacteria powder, etc., and achieve the effect of good acid resistance and bile salt resistance, ideal bacterial activity, and promoting intestinal peristalsis.

Inactive Publication Date: 2019-03-26
谭瑛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The second technical problem to be solved by the present invention is the problem that the survival rate of Streptococcus faecalis bacterial powder obtained by the freeze-dried ba

Method used

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  • Streptococcus faecium and application thereof
  • Streptococcus faecium and application thereof
  • Streptococcus faecium and application thereof

Examples

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Example Embodiment

[0031] Example 1 Isolation and screening of Streptococcus faecalis

[0032] The streptococcus faecalis with the deposit number of CGMCC No. 16128 of the present invention is isolated from human feces and obtained by the following method:

[0033] Put 1 gram of human intestinal feces into a sterile bottle containing 30 glass balls and add physiological saline to make a 100-fold dilution, and then make 10 -3 , 10 -4 , Sequentially to 10 -8 Dilution degree, take 10 -4 , 10 -5 , 10 -6 , 10 -7 , 10 -8 Five dilutions of bacterial solution were respectively inoculated on LYT Universal Agar Plate, Eosin, Meilan Agar, BB (BS) Bifidobacterium selective medium, LBS (LC) Lactobacillus selective medium, EC enterococcus selective base And Sb yeast selection base. Each medium is connected to 6 plates. Immediately after inoculation, turn the petri dish up and down to spread the inoculum evenly on the surface of the plate. Three of them were cultured aerobic and observed after 24 hours. The other...

Example Embodiment

[0049] Example 2 Preparation of freeze-dried powder of Streptococcus faecalis

[0050] The Streptococcus faecalis in this example is a strain with the deposit number CGMCC No. 16128, which is prepared as a freeze-dried powder of Streptococcus faecalis by the following method:

[0051] (1) Take the described Streptococcus faecalis, inoculate it into the primary fermentation medium according to the inoculum of 1%, and then ferment it for 12-14h under the conditions of 37±3℃ and 100-180rpm. First-level fermentation broth; then, take the first-level fermentation broth, inoculate it into the second-level fermentation medium according to the inoculum amount of 5-10%, and cultivate at a temperature of 37±3°C and a rotation speed of 100-180rpm 4-6h to obtain the secondary fermentation broth; then take the secondary fermentation broth and inoculate it into the tertiary fermentation medium at an inoculum of 5-12% at a temperature of 37±3°C and a rotation speed of 150-250rpm Under the condit...

Example Embodiment

[0055] Example 3 Preparation of freeze-dried powder of Streptococcus faecalis

[0056] The Streptococcus faecalis in this example is a strain with the deposit number CGMCC No. 16128, which is prepared as a freeze-dried powder of Streptococcus faecalis by the following method:

[0057] (1) Take the said Streptococcus faecalis and culture it in coagulated milk with an initial pH of 7.5 at a temperature of 37°C for 36 to 48 hours. As a preferred implementation of this embodiment, the said Streptococcus faecalis is 3% The inoculum is inoculated on the coagulated milk as the culture medium, and cultivated by shaking flask culture;

[0058] (2) Take the fermentation broth obtained by fermentation in step (1), place it in a centrifuge at a rotation speed of 8000r / min, centrifuge for 30min, and collect bacterial sludge;

[0059] (3) The protective agent is added to the bacterial mud obtained in step (2), and the protective agent is mixed with sucrose, skimmed milk powder and gelatin in a weig...

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Abstract

The invention provides streptococcus faecium. The streptococcus faecium is submitted to China General Microbiological Culture Collection Centre (CGMCC) for preservation on July 17, 2018; and the preservation number is CGMCC No. 16128. The streptococcus faecium provided by the invention can produce bacteriocin, and has strong capacity of inhibiting pathogenic bacteria; and moreover, the survival rate is high after the streptococcus faecium is prepared into freeze-dried powder.

Description

technical field [0001] The invention belongs to the technical field of human microecology, and in particular relates to a streptococcus faecalis and its application. Background technique [0002] Lactic acid bactria (LAB) is a general term for a class of Gram-positive bacteria that can use fermentable carbohydrates to produce a large amount of lactic acid, and Streptococcus faecalis also belongs to this category. Some scholars define lactic acid bacteria as: Gram-positive cells, rod-shaped or spherical cells, negative catalase reaction, fermentable carbohydrates mainly produce lactic acid, facultative or obligate anaerobic bacteria. Consumption of more than 50% of glucose produces lactic acid, no endospore formation, no motility, or only a few movements. Lactic acid bacteria are widely distributed in nature and are closely related to human life. They can inhabit the intestines and other organs of humans and various animals, and some species of bacteria are pathogenic to hum...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135C12R1/46
CPCA23V2002/00A23L33/135C12N1/20C12N1/205C12R2001/46A23V2200/32
Inventor 谭瑛
Owner 谭瑛
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