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Streptococcus faecium and application thereof

A technology of Streptococcus faecalis and bacterial sludge, which is applied in the field of human microecology, can solve the problem of low survival rate of Streptococcus faecalis bacteria powder, etc., and achieve the effect of good acid resistance and bile salt resistance, ideal bacterial activity, and promoting intestinal peristalsis.

Inactive Publication Date: 2019-03-26
谭瑛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The second technical problem to be solved by the present invention is the problem that the survival rate of Streptococcus faecalis bacterial powder obtained by the freeze-dried bacterial powder process in the prior art is low, and then a kind of Streptococcus faecalis freeze-dried powder with high survival rate of bacterial strains is provided preparation method

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  • Streptococcus faecium and application thereof
  • Streptococcus faecium and application thereof
  • Streptococcus faecium and application thereof

Examples

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Effect test

Embodiment 1

[0031] The isolation and screening of embodiment 1 streptococcus faecalis

[0032] The Streptococcus faecalis whose preservation number is CGMCC No.16128 of the present invention is isolated from human feces and obtained by the following method:

[0033] Put 1 gram of human intestinal feces into a sterilized bottle containing 30 glass balls and add physiological saline to make 100-fold dilution, fully beat evenly, and then make 10 -3 、10 -4 , to 10 in turn -8 Dilution, take 10 -4 、10 -5 、10 -6 、10 -7 、10 -8 Five dilutions of the bacterial solution were inoculated on LYT general agar plate, eosin, melanin agar, BB (BS) Bifidobacterium selective medium, LBS (LC) Lactobacillus selective medium, EC Enterococcus selective medium and Sb yeast selection base. Each medium was connected to 6 plates, and the culture plate was turned up and down immediately after inoculation to spread the inoculum evenly on the surface of the plate. Three of them were cultured aerobically and ob...

Embodiment 2

[0049] The preparation of embodiment 2 streptococcus faecalis freeze-dried powder

[0050] The Streptococcus faecalis in this example is a bacterial strain with a preservation number of CGMCC No.16128, which is prepared as Streptococcus faecalis freeze-dried powder by the following method:

[0051] (1) Take the Streptococcus faecalis, inoculate it into a primary fermentation medium according to 1% inoculum size, and cultivate it in a primary fermentation medium, and ferment it for 12-14h at a temperature of 37±3°C and a rotation speed of 100-180rpm to obtain First-level fermentation liquid; then, take the first-level fermentation liquid, inoculate it into the second-level fermentation medium according to the inoculum size of 5-10%, and cultivate 4-6h to obtain the secondary fermentation liquid; then take the secondary fermentation liquid and inoculate it into the tertiary fermentation medium according to the inoculum size of 5-12%. Under the condition, culture 14-16h, obtain ...

Embodiment 3

[0055] The preparation of embodiment 3 streptococcus faecalis freeze-dried powder

[0056] The Streptococcus faecalis in this example is a bacterial strain with a preservation number of CGMCC No.16128, which is prepared as Streptococcus faecalis freeze-dried powder by the following method:

[0057] (1) Take the Streptococcus faecalis and culture it in coagulated milk with an initial pH value of 7.5 at a temperature of 37°C for 36 to 48 hours. The inoculum is inoculated onto coagulated milk as a medium, and cultured in a shake flask culture;

[0058] (2) Get the fermented liquid obtained by fermentation in step (1), place it in a centrifuge with a rotating speed of 8000r / min, centrifuge for 30min, and collect the sludge;

[0059] (3) Add the protective agent to the fungus slime obtained in step (2), and the protective agent is formed by mixing sucrose, skimmed milk powder and gelatin in a weight ratio of 5:3:1. Mix the sucrose, the skimmed milk powder, and the gelatin evenly,...

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Abstract

The invention provides streptococcus faecium. The streptococcus faecium is submitted to China General Microbiological Culture Collection Centre (CGMCC) for preservation on July 17, 2018; and the preservation number is CGMCC No. 16128. The streptococcus faecium provided by the invention can produce bacteriocin, and has strong capacity of inhibiting pathogenic bacteria; and moreover, the survival rate is high after the streptococcus faecium is prepared into freeze-dried powder.

Description

technical field [0001] The invention belongs to the technical field of human microecology, and in particular relates to a streptococcus faecalis and its application. Background technique [0002] Lactic acid bactria (LAB) is a general term for a class of Gram-positive bacteria that can use fermentable carbohydrates to produce a large amount of lactic acid, and Streptococcus faecalis also belongs to this category. Some scholars define lactic acid bacteria as: Gram-positive cells, rod-shaped or spherical cells, negative catalase reaction, fermentable carbohydrates mainly produce lactic acid, facultative or obligate anaerobic bacteria. Consumption of more than 50% of glucose produces lactic acid, no endospore formation, no motility, or only a few movements. Lactic acid bacteria are widely distributed in nature and are closely related to human life. They can inhabit the intestines and other organs of humans and various animals, and some species of bacteria are pathogenic to hum...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L33/135C12R1/46
CPCA23V2002/00A23L33/135C12N1/20C12N1/205C12R2001/46A23V2200/32
Inventor 谭瑛
Owner 谭瑛
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