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A kind of Lactobacillus brevis and its application

A technology of Lactobacillus brevis and bacteria slime, applied in the direction of Lactobacillus, application, bacteria, etc., can solve the problem of low survival rate of Lactobacillus brevis powder

Active Publication Date: 2020-10-23
谭瑛
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The second technical problem to be solved by the present invention is the problem that the survival rate of Lactobacillus brevis bacteria powder obtained by the freeze-dried bacteria powder process in the prior art is low, and then a kind of Lactobacillus brevis freeze-dried powder with high survival rate of bacterial strains is provided preparation method

Method used

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  • A kind of Lactobacillus brevis and its application
  • A kind of Lactobacillus brevis and its application
  • A kind of Lactobacillus brevis and its application

Examples

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Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Isolation and screening of Lactobacillus brevis

[0035] The Lactobacillus brevis with preservation number CGMCC No. 16125 is isolated by the following method:

[0036] Put 1 gram of human intestinal feces into a sterilized bottle with 30 glass balls and add physiological saline to make a 100-fold dilution, and then make a 10-3, 10-4, and 10-8 dilution in turn. take 10 -4 、10 -5 、10 -6 、10 -7 、10 -8 Five dilutions of the bacterial solution were inoculated on LYT (self-made) universal agar plate, eosin, melanin agar, BB (BS) Bifidobacterium selective medium, LBS (LC) Lactobacillus selective medium, EC Enterococcus alternative and Sb yeast alternative. Each medium was connected to 6 plates, and the culture plate was turned up and down immediately after inoculation to spread the inoculum evenly on the surface of the plate. Three of them were cultured aerobically and observed after 24 hours. The other 3 were cultured anaerobically for 48 hours. Observed b...

Embodiment 2

[0043] Embodiment 2 Preparation of Lactobacillus brevis freeze-dried powder

[0044] The Lactobacillus brevis of the present embodiment is a bacterial strain whose preservation number is CGMCC No. 16125, and it is prepared as a Lactobacillus brevis freeze-dried powder by the following method:

[0045] (1) Take the Lactobacillus brevis and inoculate it into a 50L fermenter with 35L of LYT fermentation medium according to the inoculation amount of 1%. Under the condition of Mpa, ferment 18h, obtain primary fermentation liquid in logarithmic growth phase; Then, get described primary fermentation liquid, inoculate in the fermentor that LYT fermentation medium is housed according to the inoculum size of 8%, at temperature Under the conditions of 37°C, rotating speed of 150rpm, and tank pressure of 0.05Mpa, anaerobic culture was carried out for 20 hours to obtain a secondary fermentation liquid of logarithmic growth; Inoculate a large amount into the LYT fermentation medium, under ...

Embodiment 3

[0051] Embodiment 3 Preparation of Lactobacillus brevis freeze-dried powder

[0052] The Lactobacillus brevis of the present embodiment is a bacterial strain whose preservation number is CGMCC No. 16125, and it is prepared as a Lactobacillus brevis freeze-dried powder by the following method:

[0053] (1) Take the above-mentioned Lactobacillus brevis, inoculate it into LYT fermentation medium for anaerobic culture according to the inoculum amount of 3%, under the conditions of temperature 37±3°C, rotation speed 30rpm, and tank pressure of the fermenter 0.08Mpa and fermented for 12 hours to obtain a primary fermentation liquid in the logarithmic growth phase; then, take the primary fermentation liquid and inoculate it into the LYT fermentation medium according to the inoculum size of 5%, at a temperature of 37±3°C and a rotation speed of Under the conditions of 100rpm and the tank pressure of the fermenter being 0.03Mpa, anaerobic culture was carried out for 28 hours to obtain ...

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Abstract

The present invention provides a kind of Lactobacillus brevis, which was submitted to the General Microorganism Center (CGMCC) of China Microbiological Culture Collection Management Committee (CGMCC) for preservation on July 17, 2018, and the preservation number is: CGMCC No.16125. The Lactobacillus brevis provided by the invention has the function of regulating the health of human gastrointestinal tract.

Description

technical field [0001] The invention belongs to the technical field of human microecology, and in particular relates to a lactobacillus brevis and an application thereof. Background technique [0002] Various microorganisms (bacteria) often attach to the human body from different environments, and can settle in a certain place and continue to grow and reproduce. This phenomenon is usually called "bacterial colonization". Colonized microorganisms must rely on the continuous supply of nutrients from the human body to grow and reproduce, and then to have an impact on the human body. As a beneficial bacterium in humans and animals, Lactobacillus brevis colonizes the human intestinal tract and reproductive system, and plays a role in improving the health of the gastrointestinal tract. [0003] For example, Chinese patent document CN102660477A discloses a kind of Lactobacillus and its freeze-dried bacteria powder and its application, wherein the Lactobacillus brevis CGMCC No. 576...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/04A23L33/135A23C9/123A61K35/747A61P1/12C12R1/24
CPCA61P1/12A23C9/123A23L33/135C12N1/04C12N1/20A23V2002/00A61K2035/115C12R2001/24C12N1/205A23V2400/121A23V2200/3204A23V2200/30
Inventor 谭瑛
Owner 谭瑛
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