CDs/SiO2/AuNCs ratiometric fluorescent probe, preparation method and application

A ratiometric fluorescent probe and dosage ratio technology, applied in fluorescence/phosphorescence, chemical instruments and methods, nano optics, etc., can solve problems such as false positives and low sensitivity, and achieve improved sensitivity and accuracy, high sensitivity, and low toxicity selective effect

Inactive Publication Date: 2019-04-12
WEIFANG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This single-channel fluorescence assay may provide false positive signals and have low sensitivity due to...

Method used

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  • CDs/SiO2/AuNCs ratiometric fluorescent probe, preparation method and application
  • CDs/SiO2/AuNCs ratiometric fluorescent probe, preparation method and application
  • CDs/SiO2/AuNCs ratiometric fluorescent probe, preparation method and application

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Experimental program
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Embodiment 1

[0046] A CDs / SiO 2 The preparation method of / AuNCs ratio fluorescent probe comprises the following steps:

[0047] S1, mix citric acid and ethylenediamine with deionized water, then transfer to a polytetrafluoroethylene-lined autoclave at 200 ° C for 6 hours, and obtain a light brown transparent liquid after natural cooling, and use a dialysis bag for solution dialysis 48 hours to remove excess small molecule impurities, intercept the solution in the dialysis bag to obtain a pure carbon quantum dot solution;

[0048] Wherein, the molar ratio of citric acid and ethylenediamine is 1:2, and the molecular weight of the dialysis cut-off is 10,000.

[0049] S2, sequentially add ammonia water-absolute ethanol and tetraethyl orthosilicate to the S1 carbon quantum dot solution, mix well and react for 2 hours, then add 3-aminopropyltriethoxysilane, react for 6 hours under vigorous stirring, and centrifuge Retain the precipitate, wash with absolute ethanol and ultrapure water to obtai...

Embodiment 2

[0060] A CDs / SiO 2 The preparation method of / AuNCs ratio fluorescent probe comprises the following steps:

[0061] S1, mix citric acid and ethylenediamine with deionized water, then transfer to a polytetrafluoroethylene-lined autoclave at 150°C for 12 hours, and obtain a light brown transparent liquid after natural cooling, and use a dialysis bag for solution dialysis Remove excess small molecular impurities, intercept the solution in the dialysis bag to obtain a pure carbon quantum dot solution;

[0062] Wherein, the molar ratio of citric acid and ethylenediamine is 1:6, and the molecular weight of dialysis cut-off is 8000.

[0063] S2, sequentially add ammonia water-absolute ethanol solution and tetraethyl orthosilicate to the S1 carbon quantum dot solution, mix and react for 6h, then add 3-aminopropyltriethoxysilane, and react for 12h under vigorous stirring. Centrifuge to retain the precipitate, wash with absolute ethanol and ultrapure water to obtain amino-modified sil...

Embodiment 3

[0081] A CDs / SiO 2 The preparation method of / AuNCs ratio fluorescent probe comprises the following steps:

[0082] S1, mix citric acid and ethylenediamine with deionized water, then transfer to a polytetrafluoroethylene-lined autoclave at 250 ° C for 24 hours, and obtain a light brown transparent liquid after natural cooling, and use a dialysis bag for solution dialysis Remove excess small molecular impurities, intercept the solution in the dialysis bag to obtain a pure carbon quantum dot solution;

[0083] Wherein, the molar ratio of the amount of citric acid and ethylenediamine is 1:4, and the molecular weight of the dialysis cut-off is 9000.

[0084] S2, sequentially add ammonia water-absolute ethanol and tetraethyl orthosilicate to the S1 carbon quantum dot solution, mix well and react for 5 hours, then add 3-aminopropyltriethoxysilane, react for 8 hours under vigorous stirring, and centrifuge Retain the precipitate, wash with absolute ethanol and ultrapure water to obt...

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Abstract

The invention belongs to the technical field of composite materials, and particularly relates to a CDs/SiO2/AuNCs ratiometric fluorescent probe, a preparation method and application. The preparation method comprises the following steps that after citric acid and ethanediamine are subjected to the hybrid reaction and dialysis by adopting deionized water, a carbon quantum dot solution is obtained; an ammonium hydroxide-absolute ethyl alcohol solution and tetraethoxysilane are added for a reaction, and then 3-aminopropyl triethoxysilane is added for a reaction; mixed liquid of 1-(3-dimethylaminopropyl)-3-ethyl carbon diimine hydrochloride, an N-hydroxy succinyl imine solution and an AuNCs solution with stable 11-sulfydryl undecanoic acid which are mixed uniformly is added, and after the reaction, the CDs/SiO2/AuNCs ratiometric fluorescent probe is obtained. The detection limit of the ratiometric fluorescent probe for Cu2+ is as low as 0.013 micromole/litre, the detection limit of the ratiometric fluorescent probe for alkaline phosphatase is as low as 0.05 U/L, and the practical application value is achieved on detection of the Cu2+ and the alkaline phosphatase.

Description

technical field [0001] The invention belongs to the technical field of composite materials, in particular to a CDs / SiO 2 / AuNCs ratiometric fluorescent probe, preparation method and application. Background technique [0002] Copper is an essential trace element in the human body and plays a key role in many physiological processes that depend on copper balance. However, if there is too much copper, it can cause oxidative stress and destabilize the cellular homeostasis, leading to a variety of diseases, including Wilson's, Menkes', Alzheimer's, Parkinson's, and kidney damage. In fact, due to Cu 2+ Widely used in industry and agriculture, Cu in water 2+ The monitoring of (allowed concentration is 1.3ppm, ~20μM) is a challenging problem. [0003] Alkaline phosphatase (ALP) is an important biomarker and a commonly monitored enzyme in clinical diagnosis. Alkaline phosphatase is a membrane-bound enzyme that is widely present in biological tissues and is responsible for the re...

Claims

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Application Information

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IPC IPC(8): C09K11/65C09K11/02B82Y20/00B82Y30/00B82Y40/00G01N21/64
CPCB82Y20/00B82Y30/00B82Y40/00C09K11/025C09K11/65G01N21/6428G01N21/643G01N2021/6432
Inventor 刘海舰
Owner WEIFANG UNIV OF SCI & TECH
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