CDs/SiO2/AuNCs ratiometric fluorescent probe, preparation method and application
A ratiometric fluorescent probe and dosage ratio technology, applied in fluorescence/phosphorescence, chemical instruments and methods, nano optics, etc., can solve problems such as false positives and low sensitivity, and achieve improved sensitivity and accuracy, high sensitivity, and low toxicity selective effect
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Embodiment 1
[0046] A CDs / SiO 2 The preparation method of / AuNCs ratio fluorescent probe comprises the following steps:
[0047] S1, mix citric acid and ethylenediamine with deionized water, then transfer to a polytetrafluoroethylene-lined autoclave at 200 ° C for 6 hours, and obtain a light brown transparent liquid after natural cooling, and use a dialysis bag for solution dialysis 48 hours to remove excess small molecule impurities, intercept the solution in the dialysis bag to obtain a pure carbon quantum dot solution;
[0048] Wherein, the molar ratio of citric acid and ethylenediamine is 1:2, and the molecular weight of the dialysis cut-off is 10,000.
[0049] S2, sequentially add ammonia water-absolute ethanol and tetraethyl orthosilicate to the S1 carbon quantum dot solution, mix well and react for 2 hours, then add 3-aminopropyltriethoxysilane, react for 6 hours under vigorous stirring, and centrifuge Retain the precipitate, wash with absolute ethanol and ultrapure water to obtai...
Embodiment 2
[0060] A CDs / SiO 2 The preparation method of / AuNCs ratio fluorescent probe comprises the following steps:
[0061] S1, mix citric acid and ethylenediamine with deionized water, then transfer to a polytetrafluoroethylene-lined autoclave at 150°C for 12 hours, and obtain a light brown transparent liquid after natural cooling, and use a dialysis bag for solution dialysis Remove excess small molecular impurities, intercept the solution in the dialysis bag to obtain a pure carbon quantum dot solution;
[0062] Wherein, the molar ratio of citric acid and ethylenediamine is 1:6, and the molecular weight of dialysis cut-off is 8000.
[0063] S2, sequentially add ammonia water-absolute ethanol solution and tetraethyl orthosilicate to the S1 carbon quantum dot solution, mix and react for 6h, then add 3-aminopropyltriethoxysilane, and react for 12h under vigorous stirring. Centrifuge to retain the precipitate, wash with absolute ethanol and ultrapure water to obtain amino-modified sil...
Embodiment 3
[0081] A CDs / SiO 2 The preparation method of / AuNCs ratio fluorescent probe comprises the following steps:
[0082] S1, mix citric acid and ethylenediamine with deionized water, then transfer to a polytetrafluoroethylene-lined autoclave at 250 ° C for 24 hours, and obtain a light brown transparent liquid after natural cooling, and use a dialysis bag for solution dialysis Remove excess small molecular impurities, intercept the solution in the dialysis bag to obtain a pure carbon quantum dot solution;
[0083] Wherein, the molar ratio of the amount of citric acid and ethylenediamine is 1:4, and the molecular weight of the dialysis cut-off is 9000.
[0084] S2, sequentially add ammonia water-absolute ethanol and tetraethyl orthosilicate to the S1 carbon quantum dot solution, mix well and react for 5 hours, then add 3-aminopropyltriethoxysilane, react for 8 hours under vigorous stirring, and centrifuge Retain the precipitate, wash with absolute ethanol and ultrapure water to obt...
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