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Method for separating mogroside V

A technology of Mogroside and Luo Han Guo, which is applied in the direction of chemical instruments and methods, glycoside steroids, steroids, etc., can solve the problems of unfavorable decolorization and purification treatment, low content of active ingredients, and deepening of the color of the extract, achieving less solvent residue, The effect of stable product quality and less impurities

Inactive Publication Date: 2019-04-19
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction methods of Luo Han Guo extract mainly include water extraction and alcohol extraction, followed by macroporous adsorption resin and ion exchange resin for purification, but if the temperature is too high during the extraction process, the leached impurities will increase, and the color of the extract will deepen. It is not conducive to the subsequent decolorization and purification treatment, and the obtained extract has a deep color and has a strong taste of traditional Chinese medicine and a strong after-bitterness, low content of active ingredients, large energy consumption, and high production costs.

Method used

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  • Method for separating mogroside V
  • Method for separating mogroside V

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Take 1kg of ripe and fresh Luo Han Guo, crush it, add 3kg of pure water and beat it with a beater. Adjust the pH to 4.0 with 3% citric acid, add 0.5 ml of cellulase, and perform enzymatic hydrolysis at 40°C for 1 hour. Through a 100-mesh filter cloth, 3 ml of pectin lyase, 3 ml of papain, 3 ml of acid protease, 3 ml of amylase, and 3 ml of beta-galactanase were added, and the enzymolysis was carried out at 45 ° C for 1 hour to obtain Enzymolysis solution, heat the enzymolysis solution to 80°C, keep it for 5 minutes to inactivate the enzyme, let it cool to 25°C, add 0.5g of high-activity yeast powder (purchased from Angel Yeast (Chongzuo) Co., Ltd.), under aerobic conditions Ferment for 3 hours. Pass the fermentation liquid through an organic membrane, first pass through an organic membrane with a molecular weight of 5000, and the membrane inlet pressure is 0.45MPa, and then pass through an organic membrane with a molecular weight of 400, and the membrane inlet pressure...

Embodiment 2

[0029] Take 1kg of ripe and fresh Luo Han Guo, crush it, add 4kg of pure water and beat it with a beater. Adjust the pH to 4.5 with 4% citric acid, first add 0.5 ml of hemicellulase, 0.5 ml of β-glucanase, and 0.5 ml of pectinase, and perform enzymatic hydrolysis at 42° C. for 3 hours. Continue to add 0.5ml of pectin lyase through a 200-mesh filter cloth, enzymolyze at 48°C for 2 hours to obtain an enzymatic hydrolysis solution, heat the enzymatic hydrolysis solution to 83°C, keep it for 8 minutes to inactivate the enzyme, and let it cool to 27°C , add 1 g of high active yeast powder (purchased from Angel Yeast (Chongzuo) Co., Ltd.), and ferment for 4 hours under aerobic conditions. Pass the fermentation liquid through an organic membrane, first pass through an organic membrane with a molecular weight of 5000, the membrane inlet pressure is 0.48MPa, and then pass through an organic membrane with a molecular weight of 400, and the membrane inlet pressure is 1.9MPa to obtain a d...

Embodiment 3

[0031] Take 1kg of ripe and fresh Luo Han Guo, crush it, add 5kg of pure water and beat it with a beater. Use 5% citric acid to adjust the pH to 4.8, first add 0.6ml of cellulase, 0.6ml of hemicellulase, 0.6ml of β-glucanase, 0.6ml of pectinase, and 0.6ml of cellulase, a total of five enzymes , add a total of 3ml, and enzymatically hydrolyze at 45°C for 5 hours. Through a 200-mesh filter cloth, continue to add papain 1ml, acid protease 1ml, amylase 1ml, a total of three enzymes, and enzymolyze at 50°C for 3 hours to obtain an enzymatic hydrolysis solution. Heat the enzymatic hydrolysis solution to 85°C and keep it for 10 minutes Inactivate the enzyme, let it cool to 30°C, add 5g of high-activity yeast powder (purchased from Angel Yeast (Chongzuo) Co., Ltd.), and ferment for 5 hours under aerobic conditions. Pass the fermentation liquid through an organic membrane, first pass through an organic membrane with a molecular weight of 5000, and the membrane inlet pressure is 0.5MPa...

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Abstract

The invention relates to the field of extraction and purification of plant effective components, and especially provides a method for separating mogroside V from grosvenor momordica fruits. The methodfor separating mogroside V comprises the following steps: addition of water to grosvenor momordica fruits, pulping, phytoenzyme enzymatic hydrolysis, biological enzyme hydrolysis, collection of the obtained enzymatic hydrolysate, enzyme deactivation, yeast fermentation, composite anion-cation exchange resin purification, and concentration drying for preparing the mogroside V. The product preparedthrough the production method has the advantages of snowy white color, no bitterness, small solvent residual, no pesticide residue and stable quality; and the content of the mogroside V in the product is greater than 60%, and total glycoside content of the product is greater than 90%.

Description

technical field [0001] The invention relates to the field of extracting and purifying plant active ingredients, and in particular provides a method for isolating mogroside V from Luo Han Guo. Background technique [0002] Mangosteen, known as the "fairy fruit", is a precious and precious Cucurbitaceae plant unique to my country, and is known as a good medicine and good fruit. It has the functions of clearing away heat and moistening lung, relieving cough and relieving sore throat. The main component of Luo Han Guo extract is mogroside. The active ingredients in mogroside are mainly terpenoids, including glycoside III, glycoside IV, glycoside V, etc. Among them, mogroside V is the main component of its sweetness, and its sweetness is 256-344 times that of sucrose. Due to its combination It has a sugar group and is easily soluble in polar solvents such as water and ethanol, but hardly soluble in organic solvents such as acetone and ethyl acetate. It is a white amorphous powd...

Claims

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Application Information

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IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 赵军杨文国谢永富宋云飞
Owner GUILIN NATURAL INGREDIENTS CORP
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