Method for separating mogroside V

A technology of Mogroside and Luo Han Guo, which is applied in the direction of chemical instruments and methods, glycoside steroids, steroids, etc., can solve the problems of unfavorable decolorization and purification treatment, low content of active ingredients, and deepening of the color of the extract, achieving less solvent residue, The effect of stable product quality and less impurities

Inactive Publication Date: 2019-04-19
GUILIN NATURAL INGREDIENTS CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction methods of Luo Han Guo extract mainly include water extraction and alcohol extraction, followed by macroporous adsorption resin and ion exchange resin for purification, but if the temperature is too high during the extraction process, the leached impurities will increase, and the color of

Method used

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  • Method for separating mogroside V
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Examples

Experimental program
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Example Embodiment

[0026] Example 1

[0027] Take 1kg of ripe and fresh Luo Han Guo, crush it, add 3kg of pure water and beat with a beater. Adjust the pH to 4.0 with 3% citric acid, add 0.5ml of cellulase first, and enzymatically hydrolyze at 40°C for 1 hour. Through a 100 mesh filter cloth, continue to add 3 ml of pectin lyase, 3 ml of papain, 3 ml of acid protease, 3 ml of amylase, and 3 ml of β-galactanase, and enzymatically hydrolyze at 45°C for 1 hour to obtain Enzymatic hydrolysis solution, the enzymatic hydrolysis solution is heated to 80℃, keep it for 5 minutes to inactivate the enzyme, let it cool to 25℃, add 0.5g high-activity yeast powder (purchased from Angel Yeast (Chongzuo) Co., Ltd.), under aerobic conditions Ferment for 3 hours. Pass the fermentation broth through the organic membrane, firstly through the 5000 molecular weight organic membrane, the membrane pressure is 0.45MPa, and then through the 400 molecular weight organic membrane, the membrane pressure is 1.8MPa, and the de...

Example Embodiment

[0028] Example 2

[0029] Take 1kg of ripe and fresh Luo Han Guo, crush it, add 4kg of pure water and beat with a beater. Adjust the pH to 4.5 with 4% citric acid, first add 0.5 ml hemicellulase, 0.5 ml β-glucanase, and 0.5 ml pectinase, and enzymatically hydrolyze at 42°C for 3 hours. Add 0.5ml of pectin lyase through a 200-mesh filter cloth, and enzymolyze at 48°C for 2 hours to obtain an enzymatic hydrolysate. Heat the enzymatic hydrolysate to 83°C and keep it for 8 minutes to inactivate the enzyme, and let it cool to 27°C. , Add 1g of high-activity yeast powder (purchased from Angel Yeast (Chongzuo) Co., Ltd.), and ferment for 4 hours under aerobic conditions. Pass the fermentation broth through the organic membrane, firstly through the 5000 molecular weight organic membrane, the membrane pressure is 0.48MPa, and then through the 400 molecular weight organic membrane, the membrane pressure is 1.9MPa, and the decolorizing membrane fluid is obtained; the membrane fluid directl...

Example Embodiment

[0030] Example 3

[0031] Take 1kg of ripe and fresh Luo Han Guo, crush it, add 5kg of pure water and beat with a beater. Adjust the pH to 4.8 with 5% citric acid, first add cellulase 0.6ml, hemicellulase 0.6ml, β-glucanase 0.6ml, pectinase 0.6ml, cellulose hydrolase 0.6ml total five kinds of enzymes , Add a total of 3ml, enzymatically digest at 45°C for 5 hours. Pass through a 200 mesh filter cloth and continue to add 1ml of papain, 1ml of acid protease, and 1ml of amylase. The enzyme hydrolyses at 50°C for 3 hours to obtain an enzymatic hydrolysate. The enzymatic hydrolysate is heated to 85°C and kept for 10 minutes. Inactivate the enzyme, let it cool to 30°C, add 5 g of highly active yeast powder (purchased from Angel Yeast (Chongzuo) Co., Ltd.), and ferment for 5 hours under aerobic conditions. Pass the fermentation broth through the organic membrane, firstly through the 5000 molecular weight organic membrane, the membrane pressure is 0.5MPa, and then through the 400 molecu...

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Abstract

The invention relates to the field of extraction and purification of plant effective components, and especially provides a method for separating mogroside V from grosvenor momordica fruits. The methodfor separating mogroside V comprises the following steps: addition of water to grosvenor momordica fruits, pulping, phytoenzyme enzymatic hydrolysis, biological enzyme hydrolysis, collection of the obtained enzymatic hydrolysate, enzyme deactivation, yeast fermentation, composite anion-cation exchange resin purification, and concentration drying for preparing the mogroside V. The product preparedthrough the production method has the advantages of snowy white color, no bitterness, small solvent residual, no pesticide residue and stable quality; and the content of the mogroside V in the product is greater than 60%, and total glycoside content of the product is greater than 90%.

Description

technical field [0001] The invention relates to the field of extracting and purifying plant active ingredients, and in particular provides a method for isolating mogroside V from Luo Han Guo. Background technique [0002] Mangosteen, known as the "fairy fruit", is a precious and precious Cucurbitaceae plant unique to my country, and is known as a good medicine and good fruit. It has the functions of clearing away heat and moistening lung, relieving cough and relieving sore throat. The main component of Luo Han Guo extract is mogroside. The active ingredients in mogroside are mainly terpenoids, including glycoside III, glycoside IV, glycoside V, etc. Among them, mogroside V is the main component of its sweetness, and its sweetness is 256-344 times that of sucrose. Due to its combination It has a sugar group and is easily soluble in polar solvents such as water and ethanol, but hardly soluble in organic solvents such as acetone and ethyl acetate. It is a white amorphous powd...

Claims

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Application Information

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IPC IPC(8): C07J17/00
CPCC07J17/005
Inventor 赵军杨文国谢永富宋云飞
Owner GUILIN NATURAL INGREDIENTS CORP
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