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Preparation method and application of molecularly imprinted photo-electrochemical sensor based on N,S-CDs/CuPc composite material

A technology of molecular imprinting and composite materials, which is applied in the fields of nanomaterials, sensing, photoelectrochemical analysis and environmental monitoring, can solve the problems of poor selectivity and high detection limit, achieve cheap and portable instruments, improve selectivity, and simple preparation methods easy effect

Inactive Publication Date: 2019-05-21
HUBEI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] For the problems of high detection limit and poor selectivity of existing detection methods, the object of the present invention is to provide a non-covalent functionalized nitrogen and sulfur atom based on copper phthalocyanine (CuPc) co-doped carbon dots (N,S- CDs) complex (N,S-CDs / CuPc), using its synergistic effect to construct an enhanced photoelectrochemical molecular imprinting sensor to realize the recognition and detection of OTA toxin

Method used

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  • Preparation method and application of molecularly imprinted photo-electrochemical sensor based on N,S-CDs/CuPc composite material
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  • Preparation method and application of molecularly imprinted photo-electrochemical sensor based on N,S-CDs/CuPc composite material

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Embodiment 1

[0044] A kind of embodiment 1N of this embodiment, the preparation of S-CDs and N, S-CDs / CuPc composite material, preparation process is as follows:

[0045] (1) The carbon dots used in the present invention refer to Wang Hang-Xing et al. in the literature (Rational designof nitrogen and sulfur co-doped carbon dots for efficient photoelectrical conversion applications, Wang Hang-Xing et al, Journal of Materials ChemistryA, 2015, 3,11287) with slight modifications. Specifically, 2 g of citric acid and 1 g of L-cysteine ​​were added to deionized water in sequence, and the resulting mixture was stirred and reacted at 120°C to 130°C for 3 to 4 hours; then the obtained yellow The jelly was transferred to a polytetrafluoroethylene reactor, reacted at 180°C to 200°C for 5 to 8 hours, and then cooled to room temperature. Finally, the N,S-CDs were obtained by centrifugation and ethanol washing, rinsed, dissolved and diluted 30 times for use. Then the pH of the diluted N,S-CDs was adj...

Embodiment 2

[0046] Example 2 Preparation of molecularly imprinted photoelectrochemical sensor, the preparation process is as follows:

[0047] The molecularly imprinted polymer film used in the present invention refers to Mao Lebao et al. in the literature (Molecularly imprinted photoelectrochemical sensor for fumonisin B 1 Based on the method in GO-CdSheterojunction, Mao Lebao et al, Biosens. Bioelectron. 2019, 127, 57-63). First, mix one percent chitosan solution with complex N,S-CDs / CuPc solution, then take 100 μL of the mixed solution and drop-coat it on a clean conductive glass (ITO) electrode, and dry it at 60 °C for 2 to 3 hours and cooled to room temperature to obtain the desired complex-modified electrode. Then, 20 μL of cross-linking agent and initiator azobis, containing the original concentration of 20 mg / ml OTA template molecule, functional monomer methacrylic acid (MAA), ethylene glycol dimethacrylate (EDMA) was added dropwise on the modified electrode. The polymerization ...

Embodiment 3

[0048] Example 3 Molecularly imprinted photoelectrochemical sensor specific detection of OTA, the detection process is as follows:

[0049] Add different substances (Ochratoxin B (OTB), deoxynivalenol (DON), Zearalenone (ZON) and Patulin (PAT), Fumonisin B 1 (FB 1 ), glucose (GLU), Dopamine (DA), OTA, and a mixture containing OTA and all interfering substances were incubated, and the results were as follows image 3 As shown, the concentration of interfering substances is 10ng / mL, OTA concentration is 1ng / mL, it can be seen that the interference has no effect on the signal acquisition of the sensor, but after adding OTA, the current changes significantly, which is consistent with the sensor signal of the mixture culture, and the results show that the sensor has good selectivity for OTA.

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Abstract

The invention discloses a preparation method of a molecularly imprinted photo-electrochemical sensor based on N,S-CDs / CuPc composite material and an application in detection of ochratoxin A (OTA). Onthe basis of the synergistic effect of the co-doping of nitrogen and sulfur and non-covalent functionalization method, the energy level of the carbon point is adjusted correctly, the tight interface contact is optimized, the light absorption range is extended, and the charge transfer efficiency is enhanced to prepare the N,S-CDs / CuPc composite material; and then with combination of the molecular imprinting technology, a molecularly imprinted photo-electrochemical sensor is prepared successfully. According to the invention, the prepared sensor uses the composite material formed by N,S-CDs and CuPc as a photoelectric conversion layer; and the OTA detection is realized by surface modification of a molecularly imprinted membrane containing a toxin recognition site. The sensor has advantages ofwide detection range, high selectivity, and high sensitivity; the detection limit reaches up to 0.51pg.mL<-1>; and the sensor also has stable response and high reproducibility.

Description

technical field [0001] The invention relates to the technical field of combining nanomaterials, photoelectrochemical analysis with environmental monitoring and sensing, in particular to a preparation method of a molecularly imprinted photoelectrochemical sensor based on N,S-CDs / CuPc composite material and its application in ochratoxin A ( OTA) detection applications. Background technique [0002] Carbon dots (CDs) possess excellent optical, electrical, and photoelectrochemical properties, and are considered to be the most photoresponsive candidates for potential applications in photoelectrochemical (PEC) sensors. However, most reported CDs absorb only in the ultraviolet range. To expand their applications in photoelectrochemical sensing and analytical detection, it is necessary to explore CDs with broad spectral absorption range and high charge transfer efficiency for enhanced photocurrent response performance. According to literature reports, heteroatom doping and chemica...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/36
Inventor 毛乐宝张修华文为何汉平王升富
Owner HUBEI UNIV
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