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Molecularly imprinted stirring bar and its preparation method and application

A molecular imprinting and stirring rod technology, applied in the fields of analytical chemistry and detection, can solve the problems of complicated operation, poor selectivity, low sensitivity, etc., and achieve the effects of simplifying experimental steps, reducing emissions, and reducing matrix interference.

Active Publication Date: 2022-04-26
湖南省产商品质量监督检验研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, solid-phase microextraction is rarely used in existing methods for the detection of β-agonists in foods of animal origin
In view of the problems of cumbersome operation, poor selectivity and low sensitivity in the sample pretreatment process for the detection of β-stimulants in animal-derived foods, it is imperative to seek effective detection techniques

Method used

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  • Molecularly imprinted stirring bar and its preparation method and application
  • Molecularly imprinted stirring bar and its preparation method and application
  • Molecularly imprinted stirring bar and its preparation method and application

Examples

Experimental program
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Embodiment 1

[0050] Example 1: Molecularly imprinted stirring bar and its preparation method

[0051] A molecularly imprinted stirring rod of the present invention, the molecularly imprinted stirring rod comprises a stirring rod and a molecularly imprinted coating covering the stirring rod, the molecularly imprinted coating mainly uses clenbuterol as a template molecule and methacrylic acid as a functional unit body, ethylene glycol dimethacrylate as a cross-linking agent, methanol as a porogen, and azobisisobutyronitrile as an initiator to make a molecularly imprinted polymer, and then the template molecules in the molecularly imprinted polymer were eluted It is prepared, and the stirring rod is a glass stirring rod with an iron core whose surface is silanized.

[0052] A method for preparing the molecularly imprinted stirring rod of the above embodiment, comprising the following steps:

[0053] (1) Pretreatment of glass rods

[0054] Take a glass capillary with an outer diameter of 3 m...

Embodiment 2

[0059] Example 2: Optimization of preparation conditions for molecularly imprinted stirring bars

[0060] The present invention optimizes the preparation conditions of the molecularly imprinted stirring rod, the preparation process is the same as that of the above-mentioned Example 1, and the optimized content is as follows:

[0061] 1. Selection of polymerization reagents

[0062] In the preparation process of molecularly imprinted stirring rods, the selection of functional monomers, crosslinking agents, porogens and initiators is very important, which is directly related to the quality of the coating. After a lot of experimental analysis, we focused on the comparison of the following materials: the comparison of methacrylic acid MAA and 4-vinylpyridine (4-VP) as functional monomers, the experiment found that MAA is more conducive to the combination of functional monomers and template molecules polymerization, the prepared coating is denser and has a higher imprint ratio. T...

Embodiment 3

[0077] Example 3 SBSE process and condition optimization of molecularly imprinted stirring bar

[0078] An application method of the molecularly imprinted stirring rod of the present invention, that is, the adsorption, extraction and desorption process of the molecularly imprinted stirring rod, the molecularly imprinted stirring rod prepared by adopting the conditions of the above-mentioned Example 1 includes the following steps:

[0079] Prepare clenbuterol, ractopamine, salbutamol, terbutaline and mabuterol mixed toluene solution (sample solution 5) and place in round bottom flask 4, MIP stirring bar 6 and NIP stirring bar ( Figure 5 ) placed in the solution, at room temperature, stirred and adsorbed on the magnetic stirrer 7 at a speed of 500r / min for 1h, the schematic diagram is as follows Figure 5 shown. Then take it out, absorb the surface solution with filter paper, put it in the liner tube, add 200 μL of acetic acid solution with a volume fraction of 10%, and obtain...

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Abstract

The invention discloses a molecularly imprinted stirring rod and its preparation method and application. The molecularly imprinted stirring rod comprises a silanized surface iron-containing glass stirring rod and a molecularly imprinted coating on the glass stirring rod. The preparation method comprises the following steps: Dissolve clenbuterol in methanol, add methacrylic acid, add ethylene glycol dimethacrylate and azobisisobutyronitrile after polymerization, mix and sonicate, place a glass stirring rod in the mixed solution in a nitrogen atmosphere and a water bath React under heating to form a molecularly imprinted coating and obtain a molecularly imprinted stirring bar. The molecularly imprinted stirring rod can be used to detect β-receptor agonist drugs in animal-derived foods, which is beneficial to simplify the detection steps, reduce matrix interference, improve detection efficiency, detection accuracy and sensitivity, and is environmentally friendly and has good Scientific research value, practical application value and development prospect.

Description

technical field [0001] The invention belongs to the field of analytical chemistry and detection, and relates to a molecularly imprinted solid-phase microextraction technology and its application, in particular to a molecularly imprinted stirring rod and its preparation method and application. Background technique [0002] β-receptor agonists are a class of chemically synthesized phenylethanolamines, which can significantly increase lean meat percentage, weight gain and feed conversion of animals, thereby reducing costs. After humans eat animal tissues with a high content of β-stimulants, they will experience muscle tremors, myalgia, and even nausea, dizziness and other poisoning symptoms, which are life-threatening in severe cases. Announcement No. 235 of the Ministry of Agriculture "Maximum Residue Limits of Veterinary Drugs in Animal Foods" stipulates that such drugs shall not be detected in all animal-derived foods. Abroad, the European Union has also promulgated a serie...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 唐吉旺
Owner 湖南省产商品质量监督检验研究院