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A method for purification and separation of bacterial cellulose

A bacterial cellulose, purification and separation technology, applied in cellulose pulp post-treatment, fiber raw material treatment, papermaking, etc., can solve problems such as inability to use in-situ composite bacterial cellulose, destroy structure and performance, affect application, etc., achieve shortening The effect of purification time, short time consumption and easy operation

Inactive Publication Date: 2021-05-14
EAST CHINA JIAOTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method of using alkaline solution not only causes environmental pollution, but also consumes a lot of energy
More importantly, repeated soaking in hot alkaline solution will destroy the structure and performance of most polymer materials (such as gelatin, collagen, silk fibroin, polyurethane, polycaprolactone, polylactic acid, etc.), which makes people Composites of bacterial cellulose and commonly used biomedical polymer materials cannot be prepared by in-situ composite methods, which seriously affects its application in the biomedical field

Method used

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  • A method for purification and separation of bacterial cellulose
  • A method for purification and separation of bacterial cellulose
  • A method for purification and separation of bacterial cellulose

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for purifying and separating bacterial cellulose, comprising the following steps:

[0022] Step 1): Weigh 1.212g of Tris (Tris) powder to prepare 1000mL of 0.1M Tris solution, use HCl solution to adjust the pH to 6.5, add lysozyme powder to prepare a lysozyme with a mass volume concentration of 0.5g / L Enzyme solution; prepare 1000mL of ethylenediaminetetraacetic acid (EDTA) solution with a mass volume concentration of 1.0g / L; mix the above-mentioned lysozyme solution and EDTA solution in equal volumes to obtain a lysozyme-EDTA mixed solution;

[0023] Step 2): The bacterial cellulose hydrogel cultured for 3 days (as shown in Fig. 1(a)) was taken out and soaked in deionized water for 12 hours. Replace the deionized water and repeat it several times until the culture medium inside the bacterial cellulose is replaced by deionization, and the bacterial cellulose hydrogel appears white and opaque;

[0024] Step 3): soak the bacterial cellulose hydrogel treated in s...

Embodiment 2

[0028] A method for purifying and separating bacterial cellulose, comprising the following steps:

[0029] Step 1): Weigh 1.212g of Tris (Tris) powder to prepare 1000mL of 0.1M Tris solution, use HCl solution to adjust the pH to 6.5, add lysozyme powder to prepare a lysozyme with a mass volume concentration of 0.5g / L Enzyme solution; preparing a trypsin solution with a mass concentration of 2.5%, uniformly mixing the above-mentioned lysozyme solution and trypsin solution in equal volumes to obtain a lysozyme-trypsin mixed solution;

[0030] Step 2): The bacterial cellulose hydrogel cultured for 4 days was taken out and soaked in deionized water for 10 hours. Replace the deionized water and repeat it several times until the culture medium inside the bacterial cellulose is replaced by deionization, and the bacterial cellulose hydrogel appears white and opaque;

[0031] Step 3): The bacterial cellulose hydrogel after step 2) is soaked in the lysozyme-trypsin- Trypsin mixed solu...

Embodiment 3

[0035] A method for purifying and separating bacterial cellulose, comprising the following steps:

[0036] Step 1): prepare the lysozyme-EDTA mixed solution according to the method of Example 1, and set aside;

[0037] Step 2): The bacterial cellulose cultured for 5 days was taken out and soaked in deionized water for 12 hours. Replace the deionized water and repeat it several times until the culture medium inside the bacterial cellulose is replaced by deionization, and the bacterial cellulose hydrogel appears white and opaque;

[0038] Step 3): The bacterial cellulose hydrogel treated in step 2) is soaked in the lysozyme prepared in step 1) according to the mass volume concentration of bacterial cellulose and lysozyme-EDTA mixed solution of 0.25g / mL - in the mixed solution of EDTA; and oscillate on a shaking table, the speed is 200r / min, the temperature is 40°C, and the soaking time is 12h,

[0039] Step 4): soak the bacterial cellulose hydrogel obtained in step 3) in Span-80...

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Abstract

The invention discloses a method for purifying and separating bacterial cellulose. First, the cultured bacterial cellulose is soaked in deionized water to remove the culture medium inside the bacterial cellulose, and the bacterial cellulose is soaked in a lysozyme solution; Aminotetraacetic acid or trypsin accelerates the destruction of bacterial cell wall by lysozyme; then accelerates the rupture of bacterial cell membrane by surfactant and ultrasonic treatment to facilitate separation and removal of bacterial residues; finally, repeated washing is carried out to obtain purified bacterial cellulose. The present invention prepares purified bacterial cellulose by using lysozyme, avoiding the environmental pollution caused by alkaline substances such as sodium hydroxide or sodium carbonate and the impact on the structure and performance of the polymer material in the bacterial cellulose in-situ composite, The purified bacterial cellulose maintains the original three-dimensional shape. The invention has the advantages of simple operation, low cost, shortened purification time, green and pollution-free, easy realization of large-scale production and the like.

Description

technical field [0001] The invention belongs to the technical field of purification and separation of bacterial cellulose, and relates to a method for purification and separation of bacterial cellulose. Background technique [0002] Cellulose can be synthesized by plants, animals and microorganisms, and the cellulose synthesized by microbial fermentation is called bacterial cellulose. Bacterial cellulose is currently the thinnest natural fiber. Compared with plant cellulose, bacterial cellulose has no other substances such as lignin, pectin and hemicellulose, so bacterial cellulose has high crystallinity and excellent mechanical properties; In addition, bacterial cellulose has strong water holding capacity and good biocompatibility. These numerous advantages make it have broad application prospects in the field of biomedicine. [0003] Since the bacterial cellulose hydrogel obtained by fermentation contains many impurities and dead bodies of bacteria, the bacterial cellulo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D21C5/00D21C9/00D21C9/04
Inventor 万怡灶林钟红王捷张全超罗红林杨志伟胡剑钟美玲
Owner EAST CHINA JIAOTONG UNIVERSITY
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