Sheep embryo or sheep placenta extract, preparation method and application in cosmetics

A sheep placenta and sheep embryo technology, which is applied in the field of sheep embryo or sheep placenta extract and preparation to achieve the effects of promoting metabolism, strengthening self-growth and repair, and avoiding technological processes

Active Publication Date: 2019-06-28
CHENGDU QINGKE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a sheep embryo or sheep placenta extract, a preparation method and an application in cosmetics, which can effectively solve the limited source of human placenta existing in the prior art, some There are risks in the use of animal placenta, and the cosmetic additives provided by the invention are rich in various growth factors, which have obvious whitening, brightening, moisturizing and anti-aging effects

Method used

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  • Sheep embryo or sheep placenta extract, preparation method and application in cosmetics
  • Sheep embryo or sheep placenta extract, preparation method and application in cosmetics
  • Sheep embryo or sheep placenta extract, preparation method and application in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of Sheep Embryo or Sheep Placenta Extract

[0031] (1) Take fresh sheep mid-stage embryos, rinse their blood with physiological saline containing 200U / ml kanamycin, and place them in -20°C for cryopreservation;

[0032] (2) Take cryopreserved mid-stage sheep embryos, cut into pieces, and wash the blocky tissue with normal saline until it is flesh-colored;

[0033] (3) Pulverize the tissue block, add 1500ml of 1% sodium taurine solution per 500g of tissue, homogenize with a tissue homogenizer, and advance gradually and intermittently from 1-5 gears, and the homogenization time is 2min;

[0034] (4) Gently stir and homogenize the product obtained in step (3) at 40°C for 3 hours to make it into gruel, and adjust the pH value to 5.0;

[0035] (5) centrifuge the product obtained in step (4) at 8000r / min for 10min, take the supernatant, adjust the pH value of the supernatant to 6.3, and then heat-treat at 60°C for 10min;

[0036] (6) Centrifuge the hea...

experiment example 1

[0040] The sheep embryo extract that embodiment 1 is made is used for culturing cell, observes cell growth state, and specific process is as follows:

[0041] Experimental group: the commercially available low-sugar DMEM medium was used as the base medium, and the sheep embryo extract and vitamin C prepared in Example 1 were added. The amount of the sheep embryo extract was 0.45 mg / ml, and the amount of vitamin C was 15 μg / ml, and then inoculated the second generation of human umbilical cord mesenchymal stem cells, the inoculation volume was 1×10 5 cells / ml, subcultured in T75 flasks.

[0042] Control group: commercially available low-sugar DMEM medium was used as the basal medium, and 10% fetal bovine serum was added. The fetal bovine serum was commercially available imported fetal bovine serum, and then vitamin C was added. The amount of vitamin C added was 15 μg / ml. Then inoculated the second generation of human umbilical cord mesenchymal stem cells, the inoculum amount wa...

experiment example 2

[0059] The sheep embryo extract that embodiment 1 makes is added in the face cream as cosmetic additive, and its additive and face cream all the other components and percentage by weight are respectively: sheep placenta extract 1.0%, sodium hyaluronate 0.05%, acrylic acid ( Ester) Copolymer 0.6%, Xanthan Gum 0.2%, Cetearyl Alcohol 2.5%, Lecithin 1.0%, Butylene Glycol 3.0%, Glyceryl Polyether-263.0%, Betaine 2.0%, Luba Oil 2.0% , Niacinamide 3.0%, Panthenol 0.5%, Caprylic / Capric Triglyceride 5.0%, Isononyl Isononanoate 4.0%, Behenyl Alcohol 1.2%, Shea Butter 2.0%, Cetearyl Glucoside 0.2%, Pentaerythritol Tetraisostearate 2.0%, Acetyl Hexapeptide-8 2.0%, Yeast Lysate 1.0%, Resveratrol 1.5%, Ellagic Acid 3.0%, Collagen 2.5%, Jojoba Seed Oil 2.0%, C10-30 Acid Cholesterol / Lanosterol Mixed Ester 0.5%, Dimethicone 2.0%, Phenoxyethanol 0.5%, Tocopherol (Vitamin E) 0.1%, Disodium EDTA 0.05%, Red Bisabolol 0.1%, the balance is purified water, and its preparation process is obtained by ...

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Abstract

The invention discloses a sheep embryo or sheep placenta extract, a preparation method and application in cosmetics. The preparation method comprises the steps that sheep embryos or sheep placentas are washed, chopped, extracted with an extraction solvent and then stirred and homogenized at the temperature of 0-40 DEG C for 3-6 hours, the pH value is adjusted to be 4.9-5.0, and finally centrifugation is conducted; supernatant liquid is obtained, the pH value of the supernatant liquid is adjusted to be 6.3-6.4, and heating treatment is conducted at the temperature of 55-65 DEG C for 8-12 minutes; homogenization, centrifugation and heating treatment are conducted repeatedly 1-2 times, the treated matter is centrifuged, supernatant liquid is obtained and inactivated, the pH value is adjustedto be 7.0-7.4, package processing is conducted by using an ultrafiltration membrane, and after sterilization, the sheep embryo or sheep placenta extract is obtained. The sheep embryo or sheep placentaextract extracted by using the method contains various protein growth factors, subcutaneous tissue growth and metabolism can be promoted, therefore, the local microenvironment of the skin is activated, the self growth and repair ability of the skin is improved, and after the sheep embryo or sheep placenta extract is added into the cosmetics, the cosmetics has obvious effects of whitening, brightening and moistening the skin and resisting aging.

Description

technical field [0001] The invention relates to the technical field of placenta extract, in particular to a sheep embryo or sheep placenta extract, a preparation method and an application in cosmetics. Background technique [0002] Placenta is a commonly used traditional Chinese medicine. Modern medical research shows that placenta contains a variety of immunoglobulins, active peptides and other biologically active substances, amino acids, minerals and other components. It is a regulator of the body's immune function and a nutritional and beauty care product. Although human placenta extracts can be used as cosmetic additives, human placenta is forbidden to be bought and sold, and can only be donated, and the source is limited. Sheep placenta comes from a wide range of sources, and there is no risk of bovine protein disease transmission. Therefore, extracting active ingredients from sheep placenta and using them as cosmetic additives has broad market prospects. Contents of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/98A61K8/9789A61K8/73A61K8/64A61K8/49A61K8/44A61K8/35A61K8/34A61Q19/02A61Q19/08A61Q19/00
Inventor 陈静娴胡忠国张向飞李慧文张金龙廖知恒刘爽付春艳张学超张秋菊陈永健赵峻
Owner CHENGDU QINGKE BIOTECH
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