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Lactobacillus fermentation preparation and activating method thereof

A technology of lactic acid bacteria fermentation and activation method, applied in the field of lactic acid bacteria fermentation preparations and their activation, can solve the problems such as unstable microbial activity of live bacteria preparations, achieve improved animal palatability and growth performance, easy activation operation, stable activity and bacterial count Effect

Inactive Publication Date: 2019-07-05
大成万达(天津)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the present invention aims to propose a lactic acid bacteria fermentation preparation and its activation method to solve the problem of unstable microbial activity of live bacteria preparations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The total amount of a lactic acid bacteria fermentation preparation is 100 g, which is composed of lactic acid bacteria strains, fermentation nutrients, a quality control indicator and a protective agent.

[0024] The lactic acid bacteria strain is Lactobacillus rhamnosus 2×10 10 CFU, Lactobacillus acidophilus 2×10 10 CFU.

[0025] The fermentation nutrients include carbon source and nitrogen source, the carbon source is glucose 20%, sucrose 10%, lactose 10%, sodium citrate 12%, sodium acetate 3%; the nitrogen source is yeast extract powder 5% , 10% beef dipping powder, 20% soy peptone, 5% ammonium sulfate, and the fermented nutrients provide nutrients for the growth of lactic acid bacteria.

[0026] The quality control indicators are 0.2% bromocresol purple and 0.2% bromothymol blue, and the quality control indicators indicate the quality control of the activity of lactic acid bacteria and the number of bacteria.

[0027] The protective agent is 3% of whey powder, 0...

Embodiment 2

[0034] The total amount of a lactic acid bacteria fermentation preparation is 200 g, which is composed of lactic acid bacteria strains, fermentation nutrients, a quality control indicator and a protective agent.

[0035] The lactic acid bacteria strain is Lactobacillus plantarum 2×10 10 CFU, Lactobacillus fermentum 3×10 10 CFU, Pediococcus lactis 3×10 10 CFU, Lactobacillus casei 2×10 10 CFU.

[0036]The fermentation nutrients include carbon source and nitrogen source, the carbon source is 15% sucrose, 15% maltose, 15% soluble starch, and 4% sodium succinate; the nitrogen source is 20% beef dipping powder, tryptone 10%, soy peptone 10%, ammonium acetate 5%, the fermentation nutrients provide nutrition for the growth of lactic acid bacteria.

[0037] The quality control indicator is 0.6% litmus and 0.2% methyl orange, and the quality control indicator indicates the quality control of lactic acid bacteria activity and bacterial count.

[0038] The protective agent is skimmed...

Embodiment 3

[0044] The total amount of a lactic acid bacteria fermentation preparation is 500 g, which is composed of lactic acid bacteria strains, fermentation nutrients, a quality control indicator and a protective agent.

[0045] The lactic acid bacteria strain is Lactobacillus bulgaricus 2×10 10 CFU, Lactobacillus plantarum 2×10 10 CFU, Enterococcus faecium 2×10 10 CFU, Lactobacillus reuteri 2×10 10 CFU.

[0046] The fermentation nutrients include carbon source and nitrogen source, the carbon source is 25% glucose, 10% galactose, 2% sodium succinate, 8% sodium succinate; the nitrogen source is 20% beef dipping powder, Yeast peptone 15%, ammonium acetate 4%, ammonium chloride 4%, the fermentation nutrients provide nutrients for the growth of lactic acid bacteria.

[0047] The quality control indicators are 0.1% bromocresol violet, 0.1% methyl orange, and 0.1% bromothymol blue, and the quality control indicators indicate the quality control of lactic acid bacteria activity and bacte...

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Abstract

The invention provides a lactobacillus fermentation preparation and an activating method thereof. The lactobacillus fermentation preparation comprises lactobacillus strains, fermentation nutrients, aquality control indicator and a protecting agent. The lactobacillus fermentation preparation has the advantages that the lactobacillus fermentation preparation which is dry powder is low in transportation cost, low in water activity, capable of inhibiting the metabolic activity of microorganisms and capable of keeping the effective bacterial count of lactobacillus; the lactobacillus fermentation preparation contains the lactobacillus strains and the fermentation nutrients and is simple in activation operation, free of equipment and energy consumption costs, stable in lactobacillus activity, bacterial count and quality control and high in common user acceptability. A high-activity lactobacillus solution prepared by the activating method is mainly applicable to the inoculation of silage andbiological fermentation feed or liquid fermentation feeding system, capable of fast forming dominant strains, fast lowering material pH, generating a large amount of lactic acid and inhibiting the growth of competitors such as mildew and saccharomycetes and other harmful bacteria and also applicable to direct-feed-blending feeding, spray feeding and the like.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a lactic acid bacteria fermentation preparation and an activation method thereof. Background technique [0002] Microbial live bacteria preparation refers to the microbial live bacteria product with the number of specific (beneficial) microbial live bacteria (that is, the effective number of bacteria) as the quality index, which can be used as feed raw material starter or feed microecological additive in silage, biological fermentation feed, liquid Fermented feed, livestock and poultry soaked water health products and other breeding industries. Currently, there are two types of live microbial preparations on the market: liquid preparations and dry powder preparations. [0003] The transportation cost of liquid preparations is high, and the high water activity (aw) of liquid preparations will accelerate the metabolism and activity decline of microorganisms during s...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K30/18A23K10/18C12R1/25C12R1/225C12R1/23C12R1/46C12R1/245C12R1/01
CPCC12N1/20A23K30/18A23K10/18A23V2400/137A23V2400/147A23V2400/157A23V2400/143A23V2400/181A23V2400/173A23V2400/413A23V2400/427A23V2400/125A23V2400/123A23V2400/175A23V2400/113A23V2400/249A23V2400/169
Inventor 王晶林夏茵
Owner 大成万达(天津)有限公司
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