Method for cultivating polyploidy succulent plants

A technology for succulent plants and polyploids, which is applied in the field of plant cultivation and can solve problems such as low current intensity

Inactive Publication Date: 2019-07-12
GUANGXI NANYIZAI SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Its disadvantage is that due to the resistance of plant cell walls and cell membranes, the current inte...

Method used

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  • Method for cultivating polyploidy succulent plants
  • Method for cultivating polyploidy succulent plants

Examples

Experimental program
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Effect test

Embodiment 1

[0031] 1. Select 50 leaves of sedum succulents and cultivate them in a greenhouse. During the cultivation period, the temperature is 25°C, the sun is irradiated for 3 hours a day, soaked in rooting water for 2.5 days, and the leaves with 0.5-1.2cm adventitious roots are taken.

[0032] 2. Put the leaves into the aromatic and polyacid hydrogen composite nanofiltration membrane in the middle of the liquid MS medium, put the medium in a refrigerator at 4 °C, and induce culture for 36 hours;

[0033] 3. Get the gained blade root cell after induction in step 2), observe and screen to obtain tetraploid cells, detect tetraploid cell membrane resonance frequency with He—Ne laser Doppler effect vibrometer, gained frequency is 45 ± 2.33kHz, The output sound wave intensity is 70db. The liquid medium was energized with an alternating current with a voltage of 8V, a current of 800μA, and a frequency of 50 Hz. At the same time, it was irradiated with ultrasonic resonance for 8 days. The int...

Embodiment 2

[0037] 1. Select 50 leaves of sedum succulents and cultivate them in a greenhouse. During the cultivation period, the temperature is 28°C, 3 hours of sunlight per day, soaked in rooting water for 3 days, and the leaves with 0.5-1.2cm adventitious roots are taken.

[0038] 2. Put the leaves into the aromatic and polyacid hydrogen composite nanofiltration membrane in the middle of the liquid MS medium, put the medium in a refrigerator at 4 °C, and induce culture for 36 hours;

[0039] 3. Get the gained blade root cell after induction in step 2), observe and screen to obtain tetraploid cells, detect tetraploid cell membrane resonance frequency with He—Ne laser Doppler effect vibrometer, gained frequency is 48 ± 2.33kHz, The output sound wave intensity is 150db. The liquid medium was energized with an alternating current with a voltage of 10V, a current of 1000μA, and a frequency of 60 Hz. At the same time, it was irradiated with ultrasonic resonance for 10 days. The interval freq...

Embodiment 3

[0043] 1. Select 50 leaves of sedum succulents and cultivate them in a greenhouse. During the cultivation period, the temperature is 23°C, 3 hours of sunlight per day, soaked in rooting water for 2 days, and the leaves with 0.5-1.2cm adventitious roots are taken.

[0044] 2. Put the leaves into the aromatic and polyacid hydrogen composite nanofiltration membrane in the middle of the liquid MS medium, put the medium in a refrigerator at 4 °C, and induce culture for 36 hours;

[0045] 3. Get the gained blade root cell after induction in step 2), observe and screen to obtain tetraploid cells, detect tetraploid cell membrane resonance frequency with He—Ne laser Doppler effect vibrometer, gained frequency is 44 ± 2.33kHz, The output sound wave intensity is 20db. The liquid medium was energized with alternating current with a voltage of 5V, a current of 500μA, and a frequency of 40 Hz. At the same time, it was irradiated with ultrasonic resonance for 7 days. The interval frequency a...

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Abstract

The invention belongs to the technical field of plant cultivation, and particularly relates to a method for cultivating polyploid succulent plants. The method comprises the following specific steps: selecting a plurality of succulent leaves with adventitious roots, placing the succulent leaves into a liquid MS culture medium, then placing the succulent leaves into a refrigerating chamber at the temperature of 4 DEG C, and carrying out induction culture for 36 hours; taking tetraploid cells of the obtained leaf root cells, detecting the resonant frequency of cell membranes of the tetraploid cells, electrifying, and meanwhile, screening and culturing by using ultrasonic resonance irradiation; then culturing the obtained leaves until seedlings grow out, taking the leaves of the seedlings of the plants to detect the resonant frequency of the cell membranes, and culturing with a lignin culture medium by using ultrasonic resonance irradiation; culturing the obtained mature first-generation plant population as F1, screening F1 plants, culturing in batches to obtain a second-generation plant population F2, and selecting pure tetraploid F2 as a target seedling. Through the treatment method,the selection rate of chimeras can be improved, the rooting rate and development rate of polyploids can be improved, meanwhile, the nutrient content of plants is high, the root system is developed, and an effect of stable heredity is achieved.

Description

[0001] 【Technical field】 [0002] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for cultivating polyploid succulent plants. [0003] 【Background technique】 [0004] Polyploid breeding refers to the use of artificial mutagenesis or natural variation to obtain polyploid breeding materials by doubling the chromosome set of cells, so as to select and breed excellent varieties that meet people's needs. Polyploid refers to an individual that develops from a fertilized egg and contains three or more chromosome sets in somatic cells. The most common and effective method of polyploid breeding is the treatment of germinated seeds or seedlings with colchicine or low temperature induction. In the current technology, the polyploid acquisition process is cumbersome and time-consuming, the mutagen has a strong poisonous effect on the plant, the chimera phenomenon is serious, the pure polyploid acquisition rate is too low, and althoug...

Claims

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Application Information

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IPC IPC(8): A01H1/08A01G31/00A01G7/04C05G1/00
CPCA01G7/04A01G31/00A01H1/08C05B7/00C05C11/00C05D1/02C05D3/00C05D5/00C05D9/00C05D9/02C05F11/00C05F11/10Y02P60/14
Inventor 谢宗宜
Owner GUANGXI NANYIZAI SCI & TECH CO LTD
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