QuEChERS pretreatment method suitable for analyzing amphetamine drugs in blood

An amphetamine and blood technology, applied in the field of QuEChERS pretreatment for the analysis of amphetamines in blood, can solve the problems of incomplete phase separation, inconvenient operation, large sample amount, etc., achieve increased extraction efficiency, avoid emulsification layer, analysis good specific effect

Inactive Publication Date: 2019-07-23
SOUTH CHINA UNIV OF TECH +1
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  • Abstract
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Problems solved by technology

The above method has the following disadvantages: (1) Strict operating conditions: liquid-liquid extraction must be carried out under suitable pH conditions, so that the analyte is in a molecular state, and the pH value needs to be adjusted in SF / Z JD0107004-2016
(2) Inconvenient operation: a certain emulsified layer will be produced after liquid-liquid extraction with ether, and the phase separation is not complete enough. The separation and extraction of the supernatant layer will cause large errors due to different operators.
(3) The amount of samples is relatively large

Method used

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  • QuEChERS pretreatment method suitable for analyzing amphetamine drugs in blood
  • QuEChERS pretreatment method suitable for analyzing amphetamine drugs in blood
  • QuEChERS pretreatment method suitable for analyzing amphetamine drugs in blood

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Embodiment 1

[0040] In this embodiment, amphetamine, methamphetamine, ecstasy MDA (3,4-methylenedioxyamphetamine), MDMA (3,4-methylenedioxymethamphetamine), MDEA (N -Ethyl-3,4-methylenedioxyamphetamine), PMMA (p-methoxymethamphetamine), N-isopropylbenzylamine, Ephedrine (ephedrine), Selegiline (Selegiline), etc. Nine Standards for Amphetamines and Related Substances. The prepared standard-added samples were extracted, separated, purified and enriched by extraction reagents, and then analyzed by LC / MS-MS. Linear regression analysis was performed with the peak area (Y) of each analyte as the ordinate and the corresponding compound concentration (X, ng / mL) as the abscissa. Continuous 5d standard curve, calculate slope, intercept and deviation of correlation coefficient, linear correlation coefficient. Prepare standard supplement samples with concentrations of 100ng / mL and 400ng / mL, prepare 6 samples for each concentration on the same day, calculate the concentration and CV value, and determ...

Embodiment 2

[0057] This embodiment is an experimental example of the detection of amphetamines such as amphetamine, methamphetamine, and ecstasy in the blood of 8 drug suspects.

[0058] 1. Experimental steps:

[0059] (1) Collection of blood samples: Take the blood samples of the examiners and place them in anticoagulant blood vessels, and store them in a refrigerator at 4°C. Take 0.2mL of blood from the tested sample, add 1mL of extraction reagent containing 0.1% formic acid by volume (acetonitrile:methanol=70:30), extract at room temperature for 1 minute, then add 150mg of anhydrous magnesium sulfate, 30mg of boric acid Sodium, 4-6 grinding beads, vortex for 1 minute, then centrifuge at 5000r / min, and take the supernatant.

[0060] (2) Add 30mg of purifying agent (C18:PSA=1:2) to the supernatant, vortex for 15s~60s, let stand for 30s~90s, pass through a 0.22um organic filter membrane for nitrogen blowing, and then use acetonitrile buffer (acetonitrile : 20mmol / L ammonium acetate buff...

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Abstract

The invention discloses a QuEChERS pretreatment method suitable for analyzing amphetamine drugs in blood. According to the method, QuEChERS extraction reagent is used for effectively extracting the amphetamine drugs in the blood, and a material bag is used for purifying the complex matrix of the blood, and finally, sample detection and analysis are carried out. The method mainly comprises the steps of collecting blood samples, and extracting, separating and purifying amphetamine and related substances in the blood. The blood sample quantity required by the method can reach at least 0.2 mL, thelowest detection limit can reach 0.3-1.0 ng/mL, and the pretreatment of the single sample can be completed within 15 minutes. The method for analyzing the toxic medicine in the blood samples is simple to operate, and sample consumption is small, and in addition, the complex matrix interference in the blood is effectively eliminated, and therefore the recovery rate is greatly increased, the sensitivity is remarkably improved, and the method can be widely applied to forensic toxicant detection and forensic clinical detection.

Description

technical field [0001] The invention belongs to the field of QuEChERS pretreatment methods, in particular to a QuEChERS pretreatment method suitable for the analysis of amphetamines in blood. Background technique [0002] As the most routine biological test material in clinical practice, blood can accurately and timely reflect the drug concentration in the body and the status of each drug in the target organ, and is widely used in clinical monitoring, forensic poison analysis and other fields. In the analysis of forensic poisons, the victim's blood samples are mainly used to monitor and obtain the real-time blood drug concentration information of the parties. [0003] Blood samples contain a large amount of organic matter, including proteins (hemoglobin, plasma proteins, enzymes and protein hormones), non-protein nitrogenous compounds (nitrogenous substances in blood other than protein, mainly urea, uric acid, creatine, creatinine , amino acids, ammonia, peptides, bilirubin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88G01N2030/045
Inventor 刘洋何小维刘晓云王平陈金王羽
Owner SOUTH CHINA UNIV OF TECH
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