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Bacteriostatic substance of Bacillus amyloliquefaciens and preparation method thereof and application in preventing and treating Fusarium scabies peanut root rot

A technology for dissolving starch spores and Fusarium moniliformes, applied in the directions of botanical equipment and methods, biochemical equipment and methods, microorganism-based methods, etc., can solve the loss of peanut yield, plant withering, and environmental pollution of Fusarium moniliformes, etc. problem, to achieve efficient and stable prevention and control, and to meet the effect of prevention and control

Active Publication Date: 2022-07-29
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The pathogen lives in the soil and can survive in the soil for several years. It is a vascular parasite, which can block the ducts and secrete toxins, causing the plants to wither, resulting in a great loss of peanut yield.
[0004] At present, the prevention and control of peanut root rot can adopt reasonable crop rotation cultivation management, and chemical drugs can also be used for control, such as triadimefon, carbendazim, etc. However, due to the drug resistance of Fusarium moniliforme and the serious environmental pollution caused by chemical control, It is of great significance to carry out research on biological control of root rot

Method used

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  • Bacteriostatic substance of Bacillus amyloliquefaciens and preparation method thereof and application in preventing and treating Fusarium scabies peanut root rot
  • Bacteriostatic substance of Bacillus amyloliquefaciens and preparation method thereof and application in preventing and treating Fusarium scabies peanut root rot
  • Bacteriostatic substance of Bacillus amyloliquefaciens and preparation method thereof and application in preventing and treating Fusarium scabies peanut root rot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Screening of spores with high bacteriostatic activity against Fusarium moniliformes

[0026] (1) Screening of spores with high antibacterial activity against Fusarium moniliformes: The vinegar grain samples from the acetic acid fermentation stage of Shanxi old mature vinegar were collected and diluted and coated with MRS medium. Finally, 38 strains of spores were isolated. Bacillus 2014 with high antibacterial activity against Fusarium moniliformes CGMCC 39030 (purchased from the General Microbiology Center of China Microorganism Culture Collection Management Committee) was screened by the Oxford cup inhibition zone method.

[0027] The preparation method of above-mentioned MRS solid medium is: peptone 10g, beef extract 10g, yeast extract 5g, K 2 HPO 4 2g, Sodium Acetate 2g, Triammonium Citrate 2g, Glucose 20g, Tween 80 1g, MgSO 4 0.2g, MnSO 4 0.05g, 20g agar, 1000mL distilled water, adjust pH to 6.2‒6.6, sterilize at 121°C for 20min for use.

[0028] ...

Embodiment 2

[0033] Example 2: Preparation of Bacillus amyloliquefaciens CGMCC 15732 antibacterial substances

[0034] Activated Bacillus amyloliquefaciens CGMCC 15732 was inoculated into MRS liquid medium at 5% inoculum amount, respectively, incubated at 37°C and 160rpm / min for 24h, centrifuged at 4°C and 10000 r / min for 10 min, and the supernatant was taken. The supernatant was adjusted to neutrality with 6 mol / L HCl solution, and solid ammonium sulfate was added while stirring. When the saturation reached 80%, place in a refrigerator at 4°C overnight; after centrifugation at 4000 r / min for 20 min, the precipitate was collected and used Phosphate buffer solution with pH=7.2 was dissolved, the salt was removed by dialysis, and the antibacterial substance was obtained after vacuum freeze-drying.

[0035] The preparation method of the above-mentioned phosphate buffer is as follows: potassium dihydrogen phosphate 0.24 g, disodium hydrogen phosphate 1.42 g, sodium chloride 8.0 g, potassium ch...

Embodiment 3

[0037] Example 3: The antibacterial mechanism of Bacillus amyloliquefaciens CGMCC 15732 antibacterial substance on Fusarium moniliformes

[0038] The antibacterial mechanism of Bacillus amyloliquefaciens CGMCC 15732 against Fusarium moniliformes is mainly from the growth of mycelium, the rate of spore germination, the concentration of extracellular potassium ion, the content of extracellular AKP, the conductivity of the bacterial solution, and the morphology and cells of the mycelium. shape and so on. Depend on image 3 It can be seen that the growth delay period of Fusarium moniliformis with the antibacterial effect of Bacillus amyloliquefaciens CGMCC 15732 is longer, and the logarithmic growth period time is shorter, so it has an inhibitory effect on the mycelial growth of Fusarium moniliformes; Figure 4 It can be clearly seen that Bacillus amyloliquefaciens CGMCC 15732 has a significant inhibitory effect on the spore germination rate of Fusarium moniliformes; and has a gr...

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Abstract

The invention belongs to the technical field of microbial application, and provides a bacteriostatic substance for Bacillus amyloliquefaciens, a preparation method thereof, and an application in preventing and treating peanut root rot of Fusarium moniliformes. Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ), deposited in the General Microbiology Center of China Microorganism Culture Collection Management Committee, number: CGMCC 15732. The fermentation supernatant of the strain is subjected to ammonium sulfate precipitation, dialysis, and freeze-drying to prepare a 1.0 mg / mL antibacterial compound, which can be used in the prevention and control of peanut root rot and can reduce the effect of Fusarium moniliformes on the germination and growth of peanut seeds. In addition, the antibacterial substances have no adverse effect on peanuts, and can prevent and control the root rot disease of peanuts, and the control effect can reach 71.79%, which can better meet the requirements of peanut root rot prevention and control in agricultural production.

Description

technical field [0001] The invention belongs to the technical field of microbial application, in particular to a bacteriostatic substance of Bacillus amyloliquefaciens, a preparation method thereof, and an application in preventing and treating peanut root rot of Fusarium moniliformes. Background technique [0002] Peanut root rot can occur in all growth stages of peanuts. Peanuts are infected after sowing and before emergence, which can cause rotten seeds and rotten buds. The damage at the seedling stage causes root rot and seedling wither; the damage at the adult stage causes root rot, stem base rot and pod rot. Because the diseased parts are mainly in the roots and vascular bundles, the roots of the diseased plants become brown and rotten, the vascular bundles become brown, the main roots are shrunken and dry and rotten, and the shape is like a mouse tail. [0003] Peanut root rot is caused by infection with Fusarium species of the subphylum Deuteromycetes, including Fus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/07
CPCC12N1/20C12R2001/07C12N1/205A01N63/10
Inventor 许女贾瑞娟王愈王如福胡开燕
Owner SHANXI AGRI UNIV
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