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Compositions

A composition and drug technology, applied in the direction of drug combination, active ingredients of hydroxyl compounds, animal feed, etc., can solve the problems of limited success, prolonging chronic inflammation, preventing pro-inflammatory prostaglandins, etc.

Active Publication Date: 2019-08-16
NATUREX INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, NSAIDs not only block the synthesis of pro-inflammatory prostaglandins, these compounds also block the synthesis of anti-inflammatory prostaglandins
Thus, NSAIDs have had limited success in blocking endogenous anti-inflammatory responses and in some cases may prolong chronic inflammation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0143] Example 1: Typical extraction of Rubus extract

[0144] The extracts of the present invention are generally prepared as follows:

[0145] Rubus (aerial parts: leaves / stems) were ground such that the particles could fit through a 4 mm mesh and the ground material was mixed with EtOH / water (30% ethanol in water) in a reactor. The solvent:plant material ratio was 10:1 (v / w). The material was then extracted at reflux with stirring for about 1 hour and 30 minutes.

[0146] After extraction, the mixture was filtered using a 25 micron filter to separate the liquid from the solid phase (filter cake).

[0147] The extraction step was repeated and the resulting filtrates were combined. Discard the solid phase.

[0148] The combined filtrates were then concentrated to 50% DM under vacuum (eg 0.8 Pa).

[0149] The resulting extracted solids are referred to as "natural extracts".

[0150] The natural extract was then spray dried to a DM content of %97%.

Embodiment 2

[0151] Example 2: Determination of Dosage of Total Phenols, Hemoglobin H6 and Lambertin C in Rubus extract

[0152] The amount of various compounds in Rubus extract, including phenolic compounds such as ellagitin, lambertin C and heme h6, ellagic acid compounds such as chlorogenic acid and ellagic acid, quercetin-3- Flavonoids such as 0-xylose-glucuronide, hyperin, quercetin and kaempferol were determined using the following HPLC method.

[0153] Quantification of target compounds was performed on an Agilent 1100 HPLC system equipped with a UV detector. Compounds were separated on an Atlantis T3 C set at 32 °C 18 150mm x 3.0mm; performed on 3μm. The mobile phase consisted of acetonitrile / water (50:50) + 0.1% formic acid (eluent A) and water + 0.1% formic acid (eluent B).

[0154] The gradient of eluent A was as follows: 0 minutes, 12%; 10 minutes, 20%; 30 minutes, 43%; 40 minutes, 100%; 55 minutes, 100%.

[0155] The gradient of eluent B is the difference between 10% and ...

Embodiment 3

[0164] Example 3: Effects of Rubus chinensis on the period of inflammation resolution

[0165] Peripheral blood mononuclear cells (PBMC) were seeded and allowed to pellet for 1 hour. After 1 hour, Rubus extract was added and incubated for another 1 hour. At the end of this time, an inflammatory response was induced by PMA / A23187 in the presence of PUFA (DHA / EPA at 1 μg / ml each) for 1 hour. Culture supernatants were then removed for analysis of lipid media.

[0166] Each supernatant was concentrated by solid phase extraction (SPE) and analyzed in LC / MS. Bioactive lipids were quantified using an analytical method by linking liquid chromatography and mass spectrometry (LC / MS). LC / MS analyzes were performed on a LC 1290 Infinity (Agilent Technologies) unit coupled to a 6460 triple mass spectrometer Quad LC / MS (Agilent Technologies) equipped with an electrospray ionization source operating in the negative range (Jet Stream Technology). Chromatographic separation was carried out...

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Abstract

The present invention relates to an extract obtained from or obtainable from Rubus idaeus, a composition comprising an extract obtained from or obtainable from Rubus idaeus, processes for providing such an extract, and methods and uses relating to such extracts. In particular, the present invention relates to an extract or composition for use in treating or preventing inflammation, such as arthritis and joint inflammation.

Description

technical field [0001] The present invention relates to an extract obtained or obtainable from Rubus idaeus, a composition comprising an extract obtained or obtainable from Rubus idaeus, a method for providing such an extract and uses of such extracts. In particular, the present invention relates to extracts or compositions for the treatment or prevention of inflammations such as arthritis and joint inflammation. Background technique [0002] The listing or discussion in this specification of an apparently prior published document should not necessarily be taken as an acknowledgment that the document is part of the prior art or is common general knowledge. [0003] Inflammation is a complex biological response of tissues to noxious stimuli such as pathogens, tissue damage or irritants. This is the tissue's protective attempt to remove harmful stimuli and initiate the tissue healing process. [0004] In a typical inflammatory response, a cascade of biochemical events propa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/73A61P29/00A61P19/02
CPCA61K36/73A61K2236/00A61P29/00A61P19/02A61K31/05A61K31/192A61K31/352A23L33/105A23V2002/00A23V2200/324A23K10/30
Inventor R·莱科藏内特P·E·R·方卡-贝尔东L·D·法尔科M·特农A·C·比利M·罗莱N·弗耶尔S·比尔蒂克
Owner NATUREX INC
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