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Gold-silver nanoflower particle with core-shell structure, as well as preparation method and application thereof

A technology of core-shell structure and nanoflowers, which is applied in the field of nanomaterial preparation and biomedicine, can solve the problems of complex synthesis steps of gold nanoflower particles, limit the application of gold nanoflower particles, and cumbersome post-modification process, and achieve good photothermal conversion Efficiency, mild conditions, and the effect of enhancing Raman activity

Active Publication Date: 2019-09-13
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the synthesis methods of gold nanoflower particles reported in the literature generally have problems such as complex synthesis steps, harsh synthesis conditions, cumbersome post-modification process, and the use of biotoxic surfactants as stabilizers, which limit the use of gold nanoflower particles. Further applications in biomedicine

Method used

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  • Gold-silver nanoflower particle with core-shell structure, as well as preparation method and application thereof
  • Gold-silver nanoflower particle with core-shell structure, as well as preparation method and application thereof
  • Gold-silver nanoflower particle with core-shell structure, as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 Preparation of gold and silver nanoflower particles:

[0032] (1) Preparation of tea polyphenol-coated silver-core nanoparticles: Add 20 mg of tea polyphenol powder into a 150 mL beaker, add 15 mL of water to dissolve it, then add 176 μL of 20 mM silver nitrate solution, stir and mix evenly Finally, cover the beaker with a watch glass. As for the microwave oven, react at high temperature (100-150 ℃, 1000-1500 W) for 2 minutes. After the reaction, centrifuge at 8000 rpm for 10 minutes, and then wash with water for 3 minutes. Second, obtain silver core nanoparticles;

[0033] (2) Preparation of gold and silver nanoflower particles with a core-shell structure: Add 10 mL of the silver core nanoparticle solution obtained in step (1) into a round bottom flask, and add 400 μL of 17.6 mM chloroauric acid under rapid stirring The solution was reacted at room temperature for 5 minutes. After the reaction, it was centrifuged at 8000 rpm for 10 minutes, and then washe...

Embodiment 2

[0037] Example 2 Photothermal heating performance of gold and silver nanoflower particles

[0038] Prepare different concentrations (0-50 μg mL -1 ) of gold and silver nanoflower particle solution, placed in a quartz cuvette, the wavelength of the laser is adjusted to 808 nm, and the power is 2 watts / square centimeter. The solution was irradiated with laser light for 10 minutes. During the period, the temperature of the solution was recorded by a thermocouple probe inserted in it, and the results were as follows: Figure 4 shown. Depend on Figure 4 It can be seen that the gold and silver nanoflower particles can quickly absorb light energy and generate heat under laser irradiation, which makes the water heat up rapidly. Moreover, the greater the concentration of gold and silver nanoflower particles, the more significant the heating effect, which is expected to be used in photothermal therapy.

Embodiment 3

[0039] Example 3 Photothermal Cytotoxicity of Gold and Silver Nanoflower Particles

[0040] Preparation of gold and silver nanoflower particles modified with mercaptopolyethylene glycol 2000: Add mercaptopolyethylene glycol 2000 (purchased from Xi'an Ruixi Biotechnology Co., Ltd.) to 2 mL of 1 mg mL -1 In the gold and silver nanoflower particle solution, a mixed solution with a final concentration of mercapto polyethylene glycol 2000 of 20 mM was prepared, reacted for 12 hours, and the product was washed with water for 3 times, and finally stored at 4 °C.

[0041] The photothermal cytotoxicity of gold and silver nanoflower particles was evaluated by CCK-8 method. HeLa cells were first incubated in a 96-well plate for 24 hours, and different concentrations (0-50 μg mL -1) surface-modified gold and silver nanoflower particle solution, and then incubated for 6 hours. Afterwards, each well was irradiated with a laser for 5 minutes, and continued to incubate for 12 hours, and the...

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Abstract

The invention discloses a gold-silver nanoflower particle with a core-shell structure, as well as a preparation method and application thereof. The gold-silver nanoflower particle is quickly synthesized by adopting tea polyphenol as a reducing agent and a stabilizing agent, and no extra surface active agent and seed crystal needs to be used, so that low energy consumption and environment protection are realized. Dense multi-branched gold petals are distributed on the surface of the obtained gold-silver nanoflower particle, so that the gold-silver nanoflower particle has a remarkable near-infrared light absorption property and a favorable photo-thermal heating performance, and can be applied to photo-thermal therapy based on a near-infrared light; and meanwhile, the obtained gold-silver nanoflower particle further has an excellent surface enhanced Raman performance with good stability and is hopefully applied to Raman imaging of cells so as to have a favorable application prospects in cancer diagnosis and treatment.

Description

technical field [0001] The invention belongs to the fields of nano-material preparation and biomedicine, and in particular relates to a gold-silver nano-flower particle with a core-shell structure and a preparation method and application thereof. Background technique [0002] Cancer is a general term for a large class of malignant tumors, which has become a major global problem that endangers public health and hinders the development of the global economy. In recent years, the rise of nanomedicine has brought new opportunities for the development of new methods for efficient and targeted cancer treatment. Due to their unique optical, thermal, acoustic and magnetic properties, nanomaterials are widely used in photoimaging (such as photoacoustic imaging, optical imaging) and phototherapy (such as photothermal therapy, photodynamic therapy, photoinduced chemotherapy) A platform for the integration of disease diagnosis and treatment can be established, which can greatly improve...

Claims

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Application Information

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IPC IPC(8): B22F1/00B22F9/24B82Y30/00B82Y40/00
CPCB22F9/24B82Y30/00B82Y40/00B22F1/0553B22F1/07B22F1/054
Inventor 郑琤李娟李婧影杨黄浩
Owner FUZHOU UNIV
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