Detection method for heavy metal lead ions

A detection method, lead ion technology, applied in the field of detection of heavy metal lead ions, can solve the problems of children's blood lead exceeding the standard, complicated pre-treatment, and failure to meet the detection requirements, so as to reduce the influence, reduce the experimental error, and achieve superiority Effect

Active Publication Date: 2019-09-27
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In January 2011, more than 100 local children in Huaining County, Anhui Province had blood lead exceeding the standard. Subsequent investigations found that it was because of Borui Power Co., Ltd.’s overtime and illegal production, and the sanitary protection distance between the factory and the residential area did not meet the standard
However, in the implementation of this patent, the pretreatment of samples is relatively complicated, and a large-scale precision instrument atomic absorption instrument is required, so it cannot meet the detection requirements, and a fast and simple measurement method needs to be developed

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  • Detection method for heavy metal lead ions
  • Detection method for heavy metal lead ions
  • Detection method for heavy metal lead ions

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Embodiment 1

[0052] The present invention detects the preparation method of the ratio fluorescent biological probe of heavy metal lead ion, comprising:

[0053] In step (6), 0.5 mL of aminated magnetic beads, 2 mL of nano-gold solution, ultrasonic vibration for 3 minutes, magnetic separation to remove the supernatant, and 0.5 mL of citric acid buffer solution, the concentration of SH-DNA is 20 μM, and the amount of addition is 25.1 μL , incubate for 30 min, add 500 μL of red light CdSe@ZnS quantum dots and incubate for 2 h to prepare a red fluorescent probe. Under ultraviolet light, the solution shows red fluorescence.

[0054] In step (7), 100 μL of green CdSe@ZnS quantum dots modified with mercaptopropionic acid was added to 500 μL of DC for activation, and then mixed with 36 μL of DNA with a concentration of 20 μM terminally modified amino groups, and incubated for 2 h to prepare a green fluorescent probe. The solution showed green fluorescence.

[0055] In step (8), take 150 μL of red...

Embodiment 2

[0057] The preparation of the ratiometric fluorescent biological probe specifically includes the following steps:

[0058] Step (1), FeCl 3 ·6H 2 O is 0.81g, ethylene glycol solution is 30mL, polyacrylic acid is 20g, deionized water is 500μL and urea is 1.80g, after stirring to fully dissolve, the ultrasonic time is 10 minutes to mix, and the steel hydrotherm with polytetrafluoroethylene lining is added The volume of the kettle is 50 mL, the reaction temperature is 200° C., the reaction time is 12 hours, and then cooled to room temperature.

[0059] Step (2), get Fe in step 1.1 3 o 4 Nanomaterials are 2mL, ultrasonically dispersed, under the condition of rotating speed of 500rpm, add 0.75mL of 25% ammonia water and stir for 5 minutes, the amount of added tetraethyl orthosilicate (TEOS) is 80μL (TEOS: water ratio 1:1), The sample volume was 10 μL / 10 min and the reaction was stirred continuously at room temperature for 12 hours.

[0060]Step (3), take 500 μL of the solution...

Embodiment 3

[0064] The ratiometric fluorescent biological probe constructed by the present invention is used to detect the content of lead ions in standard samples, and construct a standard curve for detection of lead ions. The specific steps are as follows:

[0065] C-2 The ratio fluorescent bioprobe prepared by the above method is treated with the following 9 standard lead ion solutions respectively, and the molar concentrations are respectively a) 10 -5 μg / mL; b) 10 -4 μg / mL; c) 10 -3 μg / mL; d) 3×10 -3 μg / mL; e) 6×10 -3 μg / mL; f) 10 -2 μg / mL; g) 3×10 -2 μg / mL; h) 6×10 -2 μg / mL; i) 0.1 μg / mL.

[0066] At room temperature, the pH of the probe solution was adjusted to 3, 5, 7, 9, 11 respectively, and the incubation time was 0-600s. It was found that when the pH of the probe solution was 9 (such as Figure 5 ), the incubation time is 300s (such as Figure 4 ). Fluorescence Intensity I of Ratiometric Fluorescent Bioprobes 540 / I 630 The ratio of the logarithm of the lead ion conc...

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Abstract

The invention discloses a detection method of heavy metal lead ions. A ratio fluorescent biological probe is finally formed by modifying nanogold on the surface of an aminated magnetic bead to form a fluorescence quenching body, connecting the nanogold to a sulfhydrylated DNA enzyme through an Au-S bond and modifying red quantum dots to form a red fluorescent probe, and combining with a substrate chain modified with green quantum dots at the other end through base complementary pairing. Under the effect of DNA, Pb<2+> can recognize and shear the substrate chain, so that a distance between the green quantum dots and the nanogold is changed, the quenching and recovery of green fluorescence are realized, and finally, the quantitative detection of Pb<2+> is realized through the change of a red-green fluorescence ratio due to the change of the green fluorescence intensity. The method has the characteristics of simplicity and convenience, a double-signal detection mode is adopted, the influence of a background environment on a detection result is effectively reduced, and an experiment error is reduced; the method is simple and feasible to operate, low in detection cost and capable of realizing quantitative detection of a target object through a fluorescence spectrophotometer.

Description

technical field [0001] The invention relates to the technical field of chemical testing and analysis, in particular to a detection method for heavy metal lead ions. Background technique [0002] Lead is a soft and malleable heavy metal, originally blue-white in color, and poisonous. It is the most abundant and most toxic physiological toxin and neurotoxin, and it can also cause poisoning in very small amounts. The ideal lead content in human blood is 0. The accumulation of lead in the human body will disturb the nervous system, digestive system, immune system, and blood system, and even cause the death of brain cells, seriously affecting intellectual development, especially for children. More serious, there will be phenomena such as hyperactivity and learning disabilities, which will cause symptoms such as irritability, personality changes, and depression in people's personality and psychology. At present, it is mainly used in construction, lead-acid batteries, warheads, s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N1/28
CPCG01N21/6428G01N21/643G01N21/6486G01N1/28G01N2021/6432
Inventor 吴龙李明月刘静敏陈小强
Owner HUBEI UNIV OF TECH
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