Detection method of heavy metal lead ion
A detection method and lead ion technology are applied in the detection field of heavy metal lead ions, which can solve the problems of inability to meet detection requirements, children's blood lead exceeding the standard, and complicated pretreatment, so as to achieve superiority, reduce influence, and reduce experimental errors. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0052] The present invention detects the preparation method of the ratio fluorescent biological probe of heavy metal lead ion, comprising:
[0053] In step (6), 0.5 mL of aminated magnetic beads, 2 mL of nano-gold solution, ultrasonic vibration for 3 minutes, magnetic separation to remove the supernatant, and 0.5 mL of citric acid buffer solution, the concentration of SH-DNA is 20 μM, and the amount of addition is 25.1 μL , incubate for 30 min, add 500 μL of red light CdSe@ZnS quantum dots and incubate for 2 h to prepare a red fluorescent probe. Under ultraviolet light, the solution shows red fluorescence.
[0054] In step (7), 100 μL of green CdSe@ZnS quantum dots modified with mercaptopropionic acid was added to 500 μL of DC for activation, and then mixed with 36 μL of DNA with a concentration of 20 μM terminally modified amino groups, and incubated for 2 h to prepare a green fluorescent probe. The solution showed green fluorescence.
[0055] In step (8), take 150 μL of red...
Embodiment 2
[0057] The preparation of the ratiometric fluorescent biological probe specifically includes the following steps:
[0058] Step (1), FeCl 3 ·6H 2 O is 0.81g, ethylene glycol solution is 30mL, polyacrylic acid is 20g, deionized water is 500μL and urea is 1.80g, after stirring to fully dissolve, the ultrasonic time is 10 minutes to mix, and the steel hydrotherm with polytetrafluoroethylene lining is added The volume of the kettle is 50 mL, the reaction temperature is 200° C., the reaction time is 12 hours, and then cooled to room temperature.
[0059] Step (2), get Fe in step 1.1 3 o 4 Nanomaterials are 2mL, ultrasonically dispersed, under the condition of rotating speed of 500rpm, add 0.75mL of 25% ammonia water and stir for 5 minutes, the amount of added tetraethyl orthosilicate (TEOS) is 80μL (TEOS: water ratio 1:1), The sample volume was 10 μL / 10 min and the reaction was stirred continuously at room temperature for 12 hours.
[0060]Step (3), take 500 μL of the solution...
Embodiment 3
[0064] The ratiometric fluorescent biological probe constructed by the present invention is used to detect the content of lead ions in standard samples, and construct a standard curve for detection of lead ions. The specific steps are as follows:
[0065] C-2 The ratio fluorescent bioprobe prepared by the above method is treated with the following 9 standard lead ion solutions respectively, and the molar concentrations are respectively a) 10 -5 μg / mL; b) 10 -4 μg / mL; c) 10 -3 μg / mL; d) 3×10 -3 μg / mL; e) 6×10 -3 μg / mL; f) 10 -2 μg / mL; g) 3×10 -2 μg / mL; h) 6×10 -2 μg / mL; i) 0.1 μg / mL.
[0066] At room temperature, the pH of the probe solution was adjusted to 3, 5, 7, 9, 11 respectively, and the incubation time was 0-600s. It was found that when the pH of the probe solution was 9 (such as Figure 5 ), the incubation time is 300s (such as Figure 4 ). Fluorescence Intensity I of Ratiometric Fluorescent Bioprobes 540 / I 630 The ratio of the logarithm of the lead ion conc...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com