Alginate complex gel stent material constructed based on homogeneous phase crosslinking and layer-by-layer self-assembly technology and preparation method thereof

A layer-by-layer self-assembly and alginate technology, which is applied in the fields of pharmaceutical formulations, medical science, prostheses, etc., can solve the problems of lack of cell-specific binding sites, poor mechanical properties, and low gel stability, and achieve benefits Proliferation and osmotic growth, effects of good 3D topography

Inactive Publication Date: 2019-10-08
HAINAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The invention utilizes natural biomacromolecules that are naturally renewable, have large reserves, and are close in structure and performance to human bone tissue, to develop a biomacromolecule with uniform texture and regular structure, controllable degradation performance, suitable mechanical strength, and surface adhesion, recognition The alginate composite gel scaffold material based on homogeneous cross-linking and layer-by-layer self-assembly technology, which has the characteristics of induction and induction, solves the lack of cell-specific binding sites that still exist in single alginate gels in biological applications. Poor performance, low gel stability and other defects, improve its clinical applicability

Method used

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  • Alginate complex gel stent material constructed based on homogeneous phase crosslinking and layer-by-layer self-assembly technology and preparation method thereof
  • Alginate complex gel stent material constructed based on homogeneous phase crosslinking and layer-by-layer self-assembly technology and preparation method thereof
  • Alginate complex gel stent material constructed based on homogeneous phase crosslinking and layer-by-layer self-assembly technology and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] A kind of Alg / SiO 2 -The specific steps of the preparation method of the CS-GT scaffold material are:

[0054] Preparation of soaking flushing solution: prepare chitosan (CS) solution with a mass fraction of 1% and gelatin (GT) solution with a mass fraction of 1.5% in 0.005mol / L calcium chloride solution for standby.

[0055] Accurately weigh 2.0g SA and dissolve it in 100mL deionized water, and prepare a SA transparent solution with a mass fraction of 2% under electric stirring. Then add 2.0 g SiO to the solution 2 The nanoparticles and 365.2 mg of HAP powder were stirred at high speed (8000-12000 rpm, the same below) for 2 hours under the action of ultrasound to obtain a uniform mixed solution. Use a 30mL hypodermic syringe to pipette 15mL of the mixture into a 50mL beaker. Under the action of magnetic stirring, 194.4 mg GDL was added to initiate ion cross-linking, and after stirring at high speed for 2 minutes, it was quickly transferred to a 12-well tissue cultur...

Embodiment 2

[0059] A kind of Alg / SiO 2 -The specific steps of the preparation method of the CS-SP scaffold material are:

[0060] With HAP / GDL complex as cross-linking agent, SiO 2 Nanoparticles are reinforcing agents, and the alginate composite gel scaffold material is prepared through layer-by-layer assembly between biomacromolecules. In the preparation of the material, the molar ratio of HAP to GDL was fixed at 1:10, and the molar ratio of Ca in HAP to –COOH in SA was 0.18.

[0061] Preparation of immersion flushing solution: prepare CS solution with a mass fraction of 0.6% and sericin (SP) solution with a mass fraction of 1.0% in 0.01 mol / L calcium chloride solution for standby.

[0062] Under mechanical stirring and ultrasound, 1.0 g SiO 2 Nanoparticles and 182.6 mg of HAP powder were sequentially added to 100 mL of 1.5% SA solution. After it is mixed evenly, use a 30mL hypodermic syringe to pipette 15mL of the mixture into a 50mL beaker. Under magnetic stirring, 97.2 mg of GDL ...

Embodiment 3

[0065] A kind of Alg / TiO 2 -The concrete steps of the preparation method of CS-Col support material are:

[0066] with TiO 2 The nanoparticles acted as reinforcing agents, the HAP / GDL complex was the endogenous cross-linker for SA, and the EDC / NHS mixture was the covalent cross-linker for GT. And the molar ratio of HAP to GDL is 1:10, and the molar ratio of Ca in HAP to –COOH in SA is 0.36.

[0067] Preparation of immersion flushing solution: In 0.005mol / L calcium chloride solution, CS solution with a mass fraction of 0.8% and a collagen (Col) solution with a mass fraction of 1.5% were prepared for use.

[0068] 0.5g TiO 2 Nanoparticles and 365.2 mg of HAP powder were added into 100 mL of SA solution with a mass fraction of 2%, and mixed uniformly under the action of ultrasonic stirring. Then pipette 15mL of the mixture into a 50mL beaker. Under magnetic stirring, 194.4 mg of GDL was added. After high-speed stirring for 2 minutes, the mixture was quickly transferred to a...

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Abstract

The invention relates to a gel material, in particular to an alginate complex gel stent material constructed based on homogeneous phase crosslinking and layer-by-layer self-assembly technology and a preparation method thereof, and belongs to the technical field of tissue engineering. Alginate gel is prepared with an HAP / GDL compound as an endogenous crosslinking agent, wherein the mole ratio of HAP to GDL is 1:10, and meanwhile, it is determined that the mole ratio of the Ca element in the HAP to -COOH in sodium alginate (SA) is 0.18-0.36; afterwards, chitosan (CS) with positive electricity and B-type gelatin (GT) (collagen or sericin) with negative electricity are alternately deposited on the surface of the alginate gel through the electrostatic effect; and finally, the GT (collagen or sericin) on the outermost layer is subjected to covalent crosslinking through an EDC / NHS mixture, and therefore an alginate complex gel stent with homogeneous phase crosslinking is prepared.

Description

technical field [0001] The invention relates to a gel material, in particular to an alginate composite gel scaffold material constructed based on homogeneous cross-linking and layer-by-layer self-assembly technology and a preparation method thereof, belonging to the technical field of tissue engineering. Background technique [0002] Tissue engineering scaffolds aim to mimic the function of natural extracellular matrix (ECM) to generate three-dimensional (3D) tissues by stimulating and guiding cell adhesion, proliferation, and differentiation on its materials, thereby enabling the repair and regeneration of damaged tissues. To perform this function, it must have certain surface activity to promote cell adhesion, proliferation, and differentiation, sufficient mechanical strength to withstand in vivo stress and physiological loads, and a controllable degradation rate to match tissue regeneration. As an ideal biomaterial, alginate lacks cell-specific binding sites, has poor mec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/40A61L27/20A61L27/12A61L27/22A61L27/24A61L27/02A61L27/50A61L27/52A61L27/54A61L27/56A61L27/58C08J3/24C08L5/04C08L5/08C08L89/00
CPCA61L27/025A61L27/12A61L27/20A61L27/222A61L27/227A61L27/24A61L27/50A61L27/52A61L27/54A61L27/56A61L27/58A61L2300/102A61L2300/412A61L2400/12C08J3/24C08J2305/04C08J2405/08C08J2489/00C08L5/04C08L5/08C08L89/00
Inventor 颜慧琼陈秀琼林强包朝玲郝林窦文杰黄毅刘春阳
Owner HAINAN NORMAL UNIV
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