In-situ repair agent for efficient degradation of tetracycline antibiotics in soil and preparation method of the in-situ repair agent

Abstract

The invention discloses an in-situ repair agent for efficient degradation of tetracycline antibiotics in soil and a preparation method of the in-situ repair agent. The in-situ repair agent is characterized in that the in-situ repair agent is prepared from the following components in parts by weight: 30-40 parts of Pt / TiO2 modified porous silkworm excrement, 2-6 parts of graphene, 1-5 parts of cation exchange resin, 4-6 parts of methylobacterium and 4-6 parts of pseudomonas hibiscicola; the methylobacterium and the pseudomonas hibiscicola are obtained by an original strain in a basic solid medium containing tetracycline through continuous passage and domestication. The preparation process of the Pt / TiO2 modified porous silkworm excrement includes the steps that original silkworm excrement is swelled in 6-12% of a urea solution, dried, carbonized for 1-2 hours at 180-200 DEG C under the action of protective gas, quenched by being added with 0.1-1% of a bromoplatinic acid solution and tetrabutyl titanate diluents, then heated to 450-500 DEG C, and roasted for 3-5 hours. According to the in-situ repair agent for efficient degradation of the tetracycline antibiotics in the soil and thepreparation method of the in-situ repair agent, the soil polluted by the tetracycline antibiotics can be directly repaired in situ, the cycle is short, in-situ removal is carried out in combination with means of physical adsorption, photocatalytic degradation, biodegradation and the like, and the conversion rate is high.

Description

technical field [0001] The invention belongs to the technical field of contaminated soil remediation, and in particular relates to an in-situ remediation agent for efficiently degrading tetracycline antibiotics in soil and a preparation method thereof. Background technique [0002] Tetracycline antibiotics are a class of broad-spectrum antibiotics produced by actinomycetes, including aureomycin, oxytetracycline, tetracycline and semi-synthetic derivatives methacycline, doxycycline, dimethylaminotetracycline, etc. Both contain naphthalene basic skeleton. The mechanism of action of tetracycline antibiotics is to pass through the hydrophilic pores of the outer membrane, enter the cell from the energy-dependent transfer system on the inner membrane, specifically bind to the ribosomal 30S subunit, prevent aminoacyl-tRNA from linking, and thereby inhibit peptide chain elongation And protein synthesis, at the same time, it can also change the permeability of the bacterial cell mem...

AI Technical Summary

Problems solved by technology

Due to the relatively stable nature of tetracycline, it is difficult to degrade and absorb after entering the body, and more than 80% of it is discharged into the soil environment in the form of parent compounds and accumulates continuously, which induces the microorganisms in the soil to gradually develop resistance, resulting in the enrichment and resistance of drug-resistant bacteria. The mutation and production of drug genes destroy the soil environment. At the same time, tetracycline antibiotics accumulated in the soil are absorbed by crops and enter the human body through the food chain, endangering human health.

The prior art mainly repairs and degrades tetracycline antibiotic-contaminated water bodies. For example, the patent application number CN201310011876.1 discloses a phytoremediation method for tetracycline-contaminated wastewater; the patent application number CN20-1610299434.5 discloses a A preparation method of a hierarchical porous carbon material used for the adsorption of tetracycline antibiotics; such as a patent with application number CN201710610505.3, which discloses a preparation method and application of a tetracycline-contaminated water restoration material; such as a patent with application number CN20181-10103963.2 , discloses a method for degrading tetracycline in waste water by photoresponsively promoting oxidative free radicals; for example, the patent application number CN201810106340.0 discloses a preparation method and application of a chitosan magnetic tetracycline molecularly imprinted adsorbent; however, in tetracycline There are few studies on the remediation of antibiotic-like contaminated soil, and they are not deep enough. Microbial agents are mainly used for degradation, but the single biological method has a long treatment period. For example, the patent application number CN201410-348058.5 discloses mycorrhizal fungal agents and their Application in degrading residual tetracycline antibiotics; such as the patent application number CN201710115130.3, which discloses a mixed bacterial agent for degrading antibiotics in soil and its preparation method; such as the patent application number CN2017102000-22.6, which discloses a tetracycline degrading strain Bacteria and its application in the composting of livestock and poultry manure