Preparation of a gold@graphene oxide composite nanomaterial and its application in the detection of adenosine triphosphate

A technology of graphene composite and nanomaterials, applied in the detection of adenosine triphosphate, the field of preparation of gold@graphene oxide composite nanomaterials, can solve the problem of time-consuming, difficult particle size and density control of gold nanoparticles, graphene oxide High cost and other issues, to achieve excellent properties, reduce synthesis costs, and good biocompatibility

Active Publication Date: 2022-01-14
XUZHOU NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, it is difficult to control the particle size and density of gold nanoparticles formed on the surface of graphene oxide in such methods.
In addition, gold nanoparticles can also be modified on the surface of graphene oxide by NH-Au, SH-Au bonding or other bonding methods (ACS Nano, 2011, 5, 6826-6833), thereby improving the performance of gold nanoparticles on graphene oxide. The uniformity and density of the surface, however, the combination of gold nanoparticles and graphene oxide surface functional groups takes a long time, and SH- or -NH 3 Modified graphene oxide is expensive

Method used

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  • Preparation of a gold@graphene oxide composite nanomaterial and its application in the detection of adenosine triphosphate
  • Preparation of a gold@graphene oxide composite nanomaterial and its application in the detection of adenosine triphosphate
  • Preparation of a gold@graphene oxide composite nanomaterial and its application in the detection of adenosine triphosphate

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0027] Example 1. Preparation of gold@graphene oxide

[0028] 1.1 Preparation of electropositive gold nanoparticles

[0029] First, weigh 0.0364g of cetyltrimethylammonium bromide (CTAB) and dissolve it in 10mL of ultrapure water, and dissolve CTAB at 30°C; weigh 0.03938g of chloroauric acid and dissolve it in 10mL of ultrapure water , after all dissolved, dilute 10 times for later use; weigh 0.0378g of sodium borohydride and dissolve it in 10mL of ice-water mixture. Pipette 2 mL of CTAB solution and 15 mL of chloroauric acid solution in a glass beaker, stir at room temperature on a magnetic stirrer, start heating in a water bath after stirring for 15 minutes and gradually heat up to boiling, add 2.0 mL of sodium borohydride solution dropwise during heating, and Continue heating and stirring with magnetic force in a boiling state until the color of the solution changes to wine red, that is, positively charged gold nanoparticles are prepared. Such as figure 1 As shown, the a...

Embodiment 2

[0034] Example 2. Investigation of fluorescence quenching of gold@graphene oxide

[0035] In order to prove the excellent fluorescence quenching properties of gold@graphene oxide on silver nanoclusters, the present invention establishes a time-response curve. image 3 A and image 3 B respectively investigated 0, 5, 10, 15, 20, 30 μg / mL gold@graphene oxide (a-f) and 0, 10, 20, 30, 40, 50 μg / mL graphene oxide (a-f) on 50nmol / L silver Fluorescence quenching map of nanoclusters. Such as image 3 As shown in A, when the concentration of gold@graphene oxide reaches 15 μg / mL, the maximum fluorescence quenching rate (86.7%) for silver nanoclusters is achieved, while 50 μg / mL graphene oxide can only achieve 72.2% for silver nanoclusters. % Quenching ( image 3 B), the experimental results show that the functionalization of gold nanoparticles on the surface of graphene oxide greatly increases the specific surface area, which is conducive to more adsorption of silver nanocluster pro...

Embodiment 3

[0036] Example 3. Gold@Graphene Oxide for Quantitative Detection of Adenosine Triphosphate

[0037] First, take nine 1.5mL centrifuge tubes, add 10 μL of silver nanoclusters with a concentration of 10 μmol / L, 6 μL of gold@graphene oxide with a concentration of 1.0 mg / mL, and 10 μL of adenosine triphosphate ( The final concentrations were 0, 5.0pmol / L, 50pmol / L, 200pmol / L, 500pmol / L, 1.0nmol / L, 1.5nmol / L, 2.5nmol / L and 5.0nmol / L), and diluted with Tris buffer to The final volume was 400 μL. After incubation at room temperature for 40 minutes, 20 U of nuclease was added and incubated at 37° C. for 60 minutes to detect the recovery of the fluorescence of the silver nanoclusters. Such as Figure 4 As shown in A, the fluorescence recovery of silver nanoclusters has a good proportional relationship with the concentration of adenosine triphosphate, and has a good linear relationship with the concentration of adenosine triphosphate in the range of 5.0pmol / L to 2.5nmol / L, and the corr...

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Abstract

The invention discloses the preparation of a gold@graphene oxide composite nanomaterial and its application in the detection of adenosine triphosphate. Graphene aqueous solution; stir and mix a certain volume of graphene oxide aqueous solution and gold nanoparticle solution at room temperature, then ultrasonically mix, and then filter with a filter membrane; strip gold@graphene oxide from the filter membrane, and dilute it with distilled water to the desired concentration. Concentration is required for storage at low temperature. Experiments show that there is a good linear relationship between the recovery of the silver nanocluster fluorescence signal and the concentration of adenosine triphosphate in the range of 5.0 pmol / L to 2.5 nmol / L. The gold@graphene oxide composite nanomaterial prepared by the present invention can achieve a good combination of gold and graphene oxide characteristics, and has more excellent characteristics, and has good biocompatibility and high stability, and can be used as an effective fluorescence quencher Quantitative detection of biomolecules using incinerators.

Description

technical field [0001] The invention belongs to the field of clinical molecular diagnosis, and specifically relates to the preparation of a gold@graphene oxide composite nanomaterial and its application in the detection of adenosine triphosphate. Background technique [0002] Adenosine triphosphate (ATP) directly participates in the energy metabolism and biosynthesis process of living cells, and plays an important role in maintaining the normal function of organisms. In addition, ATP has also been used as an indicator of cell viability and cell damage. Therefore, the early quantitative detection of adenosine triphosphate has important application value in the food industry and clinical diagnosis. [0003] Nucleic acid aptamer sensor is a common method for detecting adenosine triphosphate. In this method, the binding principle of nucleic acid aptamer and target molecule is similar to that of antigen-antibody binding, with high specificity and strong binding force. Compared w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/65C09K11/02G01N21/64C01B32/198B22F9/24B82Y40/00B82Y30/00
CPCC09K11/65C09K11/02G01N21/6428G01N21/6486C01B32/198B22F9/24B82Y40/00B82Y30/00G01N2021/6432
Inventor 苗向敏李宗兵王颇薛宁吴淑洁
Owner XUZHOU NORMAL UNIVERSITY
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