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A kind of potassium ion probe and its preparation method and application

A technology of potassium ions and probes, applied in the field of biomaterials, to achieve the effect of improving stability

Active Publication Date: 2022-01-14
SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no sensor that can measure oxygen and potassium ion concentrations simultaneously and in real time

Method used

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  • A kind of potassium ion probe and its preparation method and application
  • A kind of potassium ion probe and its preparation method and application
  • A kind of potassium ion probe and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Synthetic example 1

[0090] This synthesis example provides the synthesis method of potassium ion probe KS-23, specifically as follows:

[0091]

[0092] (1) Synthesis of compound 2: 4-hydroxybenzaldehyde (4.8g, 0.04mol), 2-bromoethanol (4.83g, 0.06mol), KI (3.32g, 0.02mol) and K 2 CO 3 (8.29g, 0.06mol) was added to a 250ml round bottom flask, dissolved in 80ml of acetonitrile, and refluxed at 80°C for 36h. After cooling to room temperature, acetonitrile was distilled off under reduced pressure, and the residue was washed with CH 2 Cl 2 (80 mL) extracted 3 times, and washed with saturated NaCl solution (80 mL) 3 times. The organic phases were combined, dried over anhydrous magnesium sulfate, filtered, concentrated, and separated by silica gel column chromatography. The fluidity was PE (petroleum ether): EA (ethyl acetate) = 1:2 to obtain 2.5 g of a white solid with a yield of 35.3 %.

[0093] 1 H NMR (400MHz, Chloroform-d) δ9.79(s, 1H), 7.76(d, J=8.5Hz, 2H), 6.95(d, J=8.5Hz, 2H), 4.17–4.04(...

Synthetic example 2

[0102] This synthesis example provides the synthesis method of the reference probe RP, as follows:

[0103]

[0104] (1) Synthesis of Compound 5

[0105] Compound 4 (5g, 25.2mmol), 2-aminoethanol (15.4g, 252mmol) and 80ml of ethanol were added to a 150ml round bottom flask and refluxed for 10 hours and then cooled. The precipitated solid was filtered off and recrystallized from ethanol to obtain the pure product. Yield: 88.5%.

[0106] (2) Synthesis of RP

[0107] Compound 5 (1.50g, 5mmol), Et 3 N (1.01 g, 10 mmol) and 80 mL CH 2 Cl 2 Cool in an ice bath after mixing. Then methacryloyl chloride (1.04 g, 10 mmol) was added. After reacting at room temperature for 6 hours, it was concentrated in vacuo. The remaining solid was purified with a silica gel column, and a pure solid was obtained using petroleum ether / ethyl acetate (5:1) as a developing solvent. Yield 89%.

[0108] 1 H NMR (400MHz, Chloroform-d) δ8.63 (dd, J=7.3, 1.1Hz, 2H), 8.25 (dd, J=8.3, 1.1Hz, 2H), 7....

Embodiment 1

[0111] This embodiment provides a dual-functional sensing film for potassium ions and dissolved oxygen, the preparation method of which is as follows:

[0112] (1') Mix polyethylene glycol methacrylate, methacrylic acid, polyethylene glycol dimethacrylate, oxygen probe, reference probe, initiator and solvent, wherein polyethylene glycol methyl The mass ratio of acrylate, polyethylene glycol dimethacrylate, oxygen probe, reference probe, initiator and solvent is 800:50:5:5:10:1000, take 30 microliters of the mixed solution in On the TMSPA-modified quartz carrier, the upper side was covered with a cover glass, and then placed in an oven at 80 degrees for polymerization for 1 hour. After taking out, remove the cover slip to obtain a film covered with a gel layer;

[0113] (2') Place the film that step (1') obtains in the 1mLDMF solution containing 0.2mg KS-23, 100mg EDC and 20mg DMAP to carry out the grafting of potassium ion probe. The reaction was carried out after 12 hours a...

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Abstract

The invention relates to a potassium ion probe and its preparation method and application. The potassium ion probe provided by the invention has high selectivity to potassium ions, can emit green fluorescence, and detect K + Concentration, and the potassium ion probe contains hydroxyl groups, it is easier to graft with the carrier, and it can be grafted on the carrier together with other types of probes to achieve multiple responses of the same sensing film. The present invention also provides a dual-functional sensing film for potassium ions and dissolved oxygen, including a carrier, and the potassium ion probe, oxygen probe and reference probe provided by the present invention are fixed on the surface of the carrier by chemical bonding. Needle, the film has red, green and blue three-color fluorescence, and the three fluorescent colors do not interfere with each other. Fluorescence colorimetry can be used to achieve accurate and simultaneous measurement of potassium ions and dissolved oxygen, and the film has high stability.

Description

technical field [0001] The present invention relates to the technical field of biomaterials, in particular to a potassium ion probe and its preparation method and application, in particular to a potassium ion probe and its preparation method, a potassium ion and dissolved oxygen dual-functional sensing film and its preparation method . Background technique [0002] Potassium ion (K + ) is one of the most abundant metal ions in mammalian cells, accounting for about 0.35% of the dry weight of the human body. It plays an extremely important role in many physiological activities, including heart beat, muscle contraction, nerve signal conduction and renal excretion. K + It is not only involved in maintaining intracellular osmotic pressure and electrolyte balance, regulating blood pressure, but also involved in regulating the transduction of biochemical signals in the entire nervous system. K in the cytoplasm of mammalian cells + The concentration of ions is about 130-180mM, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07F5/02C09K11/06G01N21/64
CPCC07F5/022C09K11/06G01N21/6428C09K2211/1055C09K2211/1077C09K2211/1048
Inventor 田颜清刘洪甜宁爵伟
Owner SOUTH UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA
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